PMID- 29893006
OWN - NLM
STAT- MEDLINE
DCOM- 20181214
LR  - 20210109
IS  - 1098-2825 (Electronic)
IS  - 0887-8013 (Print)
IS  - 0887-8013 (Linking)
VI  - 32
IP  - 9
DP  - 2018 Nov
TI  - Evaluation of the utility of peripheral blood vs bone marrow in karyotype and 
      fluorescence in situ hybridization for myelodysplastic syndrome diagnosis.
PG  - e22586
LID - 10.1002/jcla.22586 [doi]
LID - e22586
AB  - OBJECTIVE: To clear the role of peripheral blood as a substitution for bone 
      marrow in myelodysplastic syndrome and to evaluate the concordance between 
      peripheral blood and bone marrow using karyotype and fluorescence in situ 
      hybridization (FISH) methods. METHODS: We examined 35 bone marrow (BM) and 
      peripheral blood (PB) samples from myelodysplastic syndrome (MDS) patient using 
      karyotype and FISH. Karyotype method for BM and PB samples performed using the 
      standard protocol with an exception for peripheral blood in which growth factor 
      for cultivation was not used. FISH testing was performed using a panel of 
      MDS-associated probes to detect 20q12, 20qter, 5q31, 5q33, 5p15 and chromosome 7 
      and 8 centromeres. RESULTS: Our results showed karyotypes of BM and PB are 
      concordant in 74% of cases, while about 53% of these concordances were achieved 
      from cases with normal karyotypes. However, the results of BM FISH were 
      completely concordant with PB FISH. CONCLUSION: Although peripheral blood 
      karyotype is not trustworthy for MDS diagnosis, examining peripheral blood, using 
      the FISH method, could be useful for clinical monitoring.
CI  - (c) 2018 Wiley Periodicals, Inc.
FAU - Asadi Fakhr, Zhaleh
AU  - Asadi Fakhr Z
AUID- ORCID: 0000-0001-8895-0016
AD  - Department of Genetics and Molecular Biology, Medical School, Isfahan University 
      of Medical Science, Isfahan, Iran.
FAU - Mehrzad, Valiollah
AU  - Mehrzad V
AD  - Department of Hematology and Oncology, Medical School, Omid Hospital, Isfahan 
      University of Medical Science, Isfahan, Iran.
FAU - Izaditabar, Amin
AU  - Izaditabar A
AD  - Medical Genetics Center of Genome, Isfahan, Iran.
FAU - Salehi, Mansoor
AU  - Salehi M
AUID- ORCID: 0000-0002-6565-0907
AD  - Department of Genetics and Molecular Biology, Medical School, Isfahan University 
      of Medical Science, Isfahan, Iran.
AD  - Medical Genetics Center of Genome, Isfahan, Iran.
LA  - eng
GR  - Isfahan University of Medical Sciences/
PT  - Journal Article
DEP - 20180611
PL  - United States
TA  - J Clin Lab Anal
JT  - Journal of clinical laboratory analysis
JID - 8801384
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Age Factors
MH  - Aged
MH  - Bone Marrow/*pathology
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Karyotype
MH  - Male
MH  - Middle Aged
MH  - *Myelodysplastic Syndromes/blood/diagnosis/pathology
MH  - Young Adult
PMC - PMC6817227
OTO - NOTNLM
OT  - bone marrow
OT  - fluorescence in situ hybridization
OT  - karyotype
OT  - myelodysplastic syndrome
OT  - peripheral blood
EDAT- 2018/06/13 06:00
MHDA- 2018/12/15 06:00
PMCR- 2018/06/11
CRDT- 2018/06/13 06:00
PHST- 2018/04/12 00:00 [received]
PHST- 2018/05/19 00:00 [accepted]
PHST- 2018/06/13 06:00 [pubmed]
PHST- 2018/12/15 06:00 [medline]
PHST- 2018/06/13 06:00 [entrez]
PHST- 2018/06/11 00:00 [pmc-release]
AID - JCLA22586 [pii]
AID - 10.1002/jcla.22586 [doi]
PST - ppublish
SO  - J Clin Lab Anal. 2018 Nov;32(9):e22586. doi: 10.1002/jcla.22586. Epub 2018 Jun 
      11.