PMID- 29893084
OWN - NLM
STAT- MEDLINE
DCOM- 20190211
LR  - 20190215
IS  - 1872-2075 (Electronic)
IS  - 1000-3061 (Linking)
VI  - 34
IP  - 5
DP  - 2018 May 25
TI  - [Protective effect of transplantation of bone mesenchymal stem cells on 
      demyelination in spinal cord injury].
PG  - 761-776
LID - 10.13345/j.cjb.170406 [doi]
AB  - Bone mesenchymal stem cells (BMSCs) have been used worldwide to treat spinal cord 
      injury, but their therapeutic mechanism is poorly understood. In this study, 
      BMSCs were transplanted to aneurysm clip-injured rats to demonstrate their 
      protective effect. We observed myelin sheaths through Luxol fast blue (LFB) 
      staining, osmic acid staining, TUNEL and transmission electron microscopy (TEM). 
      We performed Western blotting to analyze the expressions of brain-derived 
      neurotrophic factor (BDNF) and caspase 3. BMSCs were transplanted at 1, 7 and 14 
      days after spinal cord injury. Hindlimb movement (Basso, Beattie and Bresnahan; 
      BBB) score, CNPase (2', 3'-cyclic-nucleotide 3'-phosphodiesterase), myelin basic 
      protein (MBP) and caspase 3 protein levels were detected. Immunofluorescence was 
      used to test the differentiation of BMSCs after implanted into damaged spinal 
      cord and co-expression of CNPase-caspase 3+. At 7 days after BMSCs 
      transplantation, some injected BMSCs expressed neuronal and oligodendrocyte 
      markers. And both locomotor skills and ultra-structural features of myelin 
      sheaths were significantly improved. The expressions of BDNF were clearly 
      increased by BMSCs transplantation, the expression of caspase 3 was the opposite. 
      Compared with the 1 and 14 days transplantation after spinal cord injury, MBP and 
      CNPase expressions were highest, caspase 3 expression was lowest in 7 days BMSCs 
      transplantation. After BMSCs transplantation, CNPase-caspase 3+ cells scattered 
      in the white matter of the spinal cord. Therefore, BMSCs had a tendency to 
      differentiate into neurons and oligodendrocytes after transplantation, which 
      could promote the secretion of BDNF. BMSCs protected neural myelin sheaths by 
      inhibiting oligodendrocyte apoptosis via increased secretion of BDNF after SCI. 
      The best therapeutic time was 7 days after spinal cord injury.
FAU - Gou, Yang
AU  - Gou Y
AD  - Department of Anesthesiology, the First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400042, China.
FAU - Liu, Danyan
AU  - Liu D
AD  - Department of Anesthesiology, the First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400042, China.
FAU - Liu, Jinfeng
AU  - Liu J
AD  - Department of Anesthesiology, the First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400042, China.
FAU - Sun, Hongran
AU  - Sun H
AD  - Department of Anesthesiology, the First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400042, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sheng Wu Gong Cheng Xue Bao
JT  - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JID - 9426463
RN  - EC 3.4.22.- (Casp3 protein, rat)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Caspase 3/metabolism
MH  - Demyelinating Diseases/*therapy
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Rats
MH  - Spinal Cord Injuries/*therapy
OTO - NOTNLM
OT  - apoptosis
OT  - bone mesenchymal stem cells
OT  - cell transplantation
OT  - spinal cord injury
EDAT- 2018/06/13 06:00
MHDA- 2019/02/12 06:00
CRDT- 2018/06/13 06:00
PHST- 2018/06/13 06:00 [entrez]
PHST- 2018/06/13 06:00 [pubmed]
PHST- 2019/02/12 06:00 [medline]
AID - 10.13345/j.cjb.170406 [doi]
PST - ppublish
SO  - Sheng Wu Gong Cheng Xue Bao. 2018 May 25;34(5):761-776. doi: 
      10.13345/j.cjb.170406.