PMID- 29895936
OWN - NLM
STAT- MEDLINE
DCOM- 20191021
LR  - 20191022
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Jun 12
TI  - Int6/eIF3e Silencing Promotes Placenta Angiogenesis in a Rat Model of 
      Pre-eclampsia.
PG  - 8944
LID - 10.1038/s41598-018-27296-2 [doi]
LID - 8944
AB  - We investigated whether stable eukaryotic translation initiation factor 3e/inter 
      6 (eIF-3e/Int6) RNA-silencing (siRNA-Int6) can ameliorate pre-eclampsia (PE) by 
      promoting angiogenesis in an N-nitro-L-arginine methyl ester (L-NAME)-induced rat 
      pre-eclampsia (PE) model. Twenty-four pregnant female Sprague-Dawley rats were 
      allocated into 4 groups, including controls (Con) without any treatment, and 18 
      from gestational day (GD) 7 to GD17 L-NAME-treated rats, which were divided into 
      stable siRNA-Int6 transfected (siRNA-Int6), negative vector control siRNA 
      (NC-siRNA) and PE control (PE-Con) groups. All adenovirus siRNA transfections 
      were performed on GD7 via intravenous tail injection. On GD0, GD11 and GD17, 
      blood pressure, and on GD6 and GD17, protein estimations in 24 h urine samples 
      were conducted. All animals were sacrificed on GD18. In the PE-Con group, 
      placental Int6 was expressed to a significantly greater level than in the Con 
      group, which was reversed by the application of siRNA-Int6. Blood pressure and 
      proteinuria were significantly lower in the siRNA-Int6 group than in the PRE-Con 
      group. As shown by CD31 and IB4 expression, placental micro-vascular density 
      (MVD) was significantly higher in the siRNA-Int6 group than in the PE-Con and 
      NC-siRNA groups, which has accompanied by enhanced trophoblast invasion. Int6 
      silencing alleviated the maternal clinical manifestations of pre-eclampsia and 
      promoted placental angiogenesis in pregnant L-NAME-treated rats.
FAU - Li, Qin
AU  - Li Q
AD  - Department of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, 
      No. 128, Shenyang Road, Shanghai, 200090, China. liqinbm@163.com.
FAU - Yao, Baolin
AU  - Yao B
AD  - Department of Obstetrics, Obstetrics and Gynecology Hospital of Fudan University, 
      No. 128, Shenyang Road, Shanghai, 200090, China.
FAU - Endler, Alexander
AU  - Endler A
AD  - Department of Molecular Medical Research, Tokyo Metropolitan Institute of Medical 
      Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506, Japan.
AD  - Shanghai BIOMED Science Technology Co., Ltd, Room 2804-06 Baishu Building No. 
      1230, Zhongshan Bei Yi Road, Shanghai, 200437, China.
FAU - Chen, Li
AU  - Chen L
AD  - Department of Molecular Medical Research, Tokyo Metropolitan Institute of Medical 
      Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506, Japan.
AD  - Shanghai BIOMED Science Technology Co., Ltd, Room 2804-06 Baishu Building No. 
      1230, Zhongshan Bei Yi Road, Shanghai, 200437, China.
FAU - Shibasaki, Futoshi
AU  - Shibasaki F
AD  - Department of Molecular Medical Research, Tokyo Metropolitan Institute of Medical 
      Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506, Japan.
FAU - Cheng, Haidong
AU  - Cheng H
AD  - Department of Obstetrics, Obstetrics and Gynecology Hospital of Fudan University, 
      No. 128, Shenyang Road, Shanghai, 200090, China. hdcheng_2003@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180612
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Eukaryotic Initiation Factor-3)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - Animals
MH  - Blood Pressure/genetics/physiology
MH  - Disease Models, Animal
MH  - Eukaryotic Initiation Factor-3/*genetics/metabolism
MH  - Female
MH  - Humans
MH  - NG-Nitroarginine Methyl Ester
MH  - Neovascularization, Physiologic/*genetics/physiology
MH  - Placenta/*blood supply/metabolism
MH  - Pre-Eclampsia/chemically induced/*genetics/metabolism
MH  - Pregnancy
MH  - Proteinuria/genetics/metabolism
MH  - *RNA Interference
MH  - Rats, Sprague-Dawley
PMC - PMC5997673
COIS- The authors declare no competing interests.
EDAT- 2018/06/14 06:00
MHDA- 2019/10/23 06:00
PMCR- 2018/06/12
CRDT- 2018/06/14 06:00
PHST- 2016/06/24 00:00 [received]
PHST- 2018/05/30 00:00 [accepted]
PHST- 2018/06/14 06:00 [entrez]
PHST- 2018/06/14 06:00 [pubmed]
PHST- 2019/10/23 06:00 [medline]
PHST- 2018/06/12 00:00 [pmc-release]
AID - 10.1038/s41598-018-27296-2 [pii]
AID - 27296 [pii]
AID - 10.1038/s41598-018-27296-2 [doi]
PST - epublish
SO  - Sci Rep. 2018 Jun 12;8(1):8944. doi: 10.1038/s41598-018-27296-2.