PMID- 29896216
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1664-8021 (Print)
IS  - 1664-8021 (Electronic)
IS  - 1664-8021 (Linking)
VI  - 9
DP  - 2018
TI  - miR-155 and miR-122 Expression of Spermatozoa in Obese Subjects.
PG  - 175
LID - 10.3389/fgene.2018.00175 [doi]
LID - 175
AB  - Obesity is characterized by mild chronic inflammation that is linked with 
      impaired iron homeostasis. Studies in human and murine show that there is a 
      transgenerational epigenetic inheritance via the gametes in obesity; however, 
      there is little information on changes in the expression of microRNAs related to 
      inflammation and iron homeostasis in spermatozoa from obese subjects. The present 
      study investigated the expression of microRNAs related to inflammation (miR-21 y 
      miR-155) and iron nutrition (miR-122 and miR-200b) in plasma, peripheral blood 
      mononuclear cells (PBMC) and spermatozoa from normozoospermic controls (Cn; n = 
      17; BMI: 24.6 +/- 2.0) and obese (Ob; n = 17; BMI: 32.6 +/- 4.4) men. To determine 
      the inflammation levels, we measured IL-6, TNF-alpha, and monocyte chemoattractant 
      protein-1 (MCP1) by Magnetic Luminex((R)) Assay. mRNA expression of IL6, TNF-alpha, and 
      hepcidin (HAMP) in PBMC were evaluated by RT-qPCR. The analysis of microRNAs was 
      performed using the Taqman((R)) assays. The iron content in PBMC, seminal plasma, 
      and spermatozoa was determined by Inductively Coupled Plasma Mass Spectrometry 
      (ICP-MS). High serum IL6, TNF-alpha, and MCP1 levels were observed in Ob group (p < 
      0.05). Gene expression analysis showed an increased abundance relative of TNF-alpha 
      (p = 0.018), HAMP (p = 0.03), and IL6 (p = 0.02) in PBMC from obese subjects. 
      Also, we observed high levels of serum ferritin (p = 0.03), iron content in 
      seminal plasma (p = 0.04), and spermatozoa (p = 0.002), but lower serum Fe (p = 
      0.007) in obese subjects. In the Ob group, a high expression of miR-155 (p = 
      0.02) and miR-21 (p = 0.03) was observed in PBMC and miR-122 (p = 0.03) in 
      plasma. In sperm, both miR-155 (p = 0.004) and miR-122 (p = 0.028) were high in 
      the Ob group. Our results showed that obese subjects have increased expressions 
      of miR-155 and miR-122, two microRNAs that were previously related with 
      inflammation and iron metabolism, respectively, at both the systemic and sperm 
      levels.
FAU - Lopez, Paulina
AU  - Lopez P
AD  - Micronutrient Laboratory, Institute of Nutrition and Food Technology, University 
      of Chile, Santiago, Chile.
FAU - Castro, Andrea
AU  - Castro A
AD  - Institute of Maternal and Child Research, Faculty of Medicine, University of 
      Chile, Santiago, Chile.
FAU - Florez, Martha
AU  - Florez M
AD  - Institute of Maternal and Child Research, Faculty of Medicine, University of 
      Chile, Santiago, Chile.
FAU - Miranda, Karen
AU  - Miranda K
AD  - Micronutrient Laboratory, Institute of Nutrition and Food Technology, University 
      of Chile, Santiago, Chile.
FAU - Aranda, Pilar
AU  - Aranda P
AD  - CIBM, INYTA, IMUDS, Department of Physiology, Faculty of Pharmacy, University of 
      Granada, Granada, Spain.
FAU - Sanchez-Gonzalez, Cristina
AU  - Sanchez-Gonzalez C
AD  - CIBM, INYTA, IMUDS, Department of Physiology, Faculty of Pharmacy, University of 
      Granada, Granada, Spain.
FAU - Llopis, Juan
AU  - Llopis J
AD  - CIBM, INYTA, IMUDS, Department of Physiology, Faculty of Pharmacy, University of 
      Granada, Granada, Spain.
FAU - Arredondo, Miguel
AU  - Arredondo M
AD  - Micronutrient Laboratory, Institute of Nutrition and Food Technology, University 
      of Chile, Santiago, Chile.
LA  - eng
PT  - Journal Article
DEP - 20180529
PL  - Switzerland
TA  - Front Genet
JT  - Frontiers in genetics
JID - 101560621
PMC - PMC5986881
OTO - NOTNLM
OT  - iron
OT  - microRNAs
OT  - obesity
OT  - pro-inflammatory cytokines
OT  - spermatozoa
EDAT- 2018/06/14 06:00
MHDA- 2018/06/14 06:01
PMCR- 2018/05/29
CRDT- 2018/06/14 06:00
PHST- 2018/02/15 00:00 [received]
PHST- 2018/04/27 00:00 [accepted]
PHST- 2018/06/14 06:00 [entrez]
PHST- 2018/06/14 06:00 [pubmed]
PHST- 2018/06/14 06:01 [medline]
PHST- 2018/05/29 00:00 [pmc-release]
AID - 10.3389/fgene.2018.00175 [doi]
PST - epublish
SO  - Front Genet. 2018 May 29;9:175. doi: 10.3389/fgene.2018.00175. eCollection 2018.