PMID- 29897097
OWN - NLM
STAT- MEDLINE
DCOM- 20181224
LR  - 20190116
IS  - 1473-2262 (Electronic)
IS  - 1473-2262 (Linking)
VI  - 35
DP  - 2018 Jun 13
TI  - Improved tendon healing using bFGF, BMP-12 and TGFbeta1 in a rat model.
PG  - 318-334
LID - 10.22203/eCM.v035a22 [doi]
AB  - Several growth factors (GFs) are expressed as tendons heal, but it remains 
      unknown whether their combined application enhances the healing process. This 
      matter was addressed by applying a combination of basic fibroblast growth factor 
      (bFGF), bone morphogenetic protein 12 (BMP-12) and transforming growth factor 
      beta 1 (TGFbeta1) in a rat Achilles tendon transection model. GFs were applied in 
      one of the three following ways: i) direct application of all three factors at 
      the time of surgery; ii) sequential, tiered percutaneous injection of individual 
      factors immediately after surgery, 48 h and 96 h later; iii) load of all three 
      factors onto a collagen sponge implanted at the time of surgery. After 1, 2, 4 
      and 8 weeks, healing was assessed based on tendon length and thickness, 
      mechanical strength, stiffness and histology. Best results were achieved when GFs 
      were loaded onto a collagen sponge - with a rapid increase in mechanical strength 
      (load to failure, 71.2 N vs. 7.7 N in controls), consistent tendon length over 
      time (9.9 mm vs. 16.2 mm in controls) and faster tendon remodelling, as measured 
      by histology - followed by tiered injection therapy over 96 h. In conclusion, 
      implantation of a GF-loaded collagen sponge could provide a promising treatment, 
      especially in high-performance athletes and revision cases prone to re-rupture. 
      For conservative treatment, tiered percutaneous GF application could be an option 
      for improving clinical outcome.
FAU - Majewski, M
AU  - Majewski M
FAU - Heisterbach, P
AU  - Heisterbach P
FAU - Jaquiery, C
AU  - Jaquiery C
FAU - Durselen, L
AU  - Durselen L
FAU - Todorov, A
AU  - Todorov A
FAU - Martin, I
AU  - Martin I
FAU - Evans, C H
AU  - Evans CH
FAU - Muller, S A
AU  - Muller SA
AD  - Department of Orthopaedic Surgery, University of Basel, Spitalstrasse 21, 4031 
      Basel, Switzerland.sebastian.mueller@usb.ch.
LA  - eng
PT  - Journal Article
DEP - 20180613
PL  - United States
TA  - Eur Cell Mater
JT  - European cells & materials
JID - 100973416
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (growth differentiation factor 7)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Biomechanical Phenomena
MH  - Bone Morphogenetic Proteins/*pharmacology
MH  - Collagen/metabolism
MH  - Fibroblast Growth Factor 2/*pharmacology
MH  - Horses
MH  - Male
MH  - Rats, Sprague-Dawley
MH  - Tendons/*pathology/surgery
MH  - Transforming Growth Factor beta1/*pharmacology
MH  - Weight-Bearing
MH  - Wound Healing/*drug effects
EDAT- 2018/06/14 06:00
MHDA- 2018/12/26 06:00
CRDT- 2018/06/14 06:00
PHST- 2018/06/14 06:00 [entrez]
PHST- 2018/06/14 06:00 [pubmed]
PHST- 2018/12/26 06:00 [medline]
AID - vol035a22 [pii]
AID - 10.22203/eCM.v035a22 [doi]
PST - epublish
SO  - Eur Cell Mater. 2018 Jun 13;35:318-334. doi: 10.22203/eCM.v035a22.