PMID- 29897188
OWN - NLM
STAT- MEDLINE
DCOM- 20180820
LR  - 20181202
IS  - 0513-4870 (Print)
IS  - 0513-4870 (Linking)
VI  - 51
IP  - 7
DP  - 2016 Jul
TI  - [Exposure of human hepatoma cells to nitrite and ammonia promotes invasive 
      activity through activation of ROS/ODC pathway].
PG  - 1083-90
AB  - Recent studies have demonstrated that nitrite and ammonia levels are higher in 
      the tumor environment, but their effects on cancer cells remains unclear. The 
      present study was designed to determine the effects of nitrite and ammonia on 
      tumor invasion and the role of reactive oxygen (ROS)/ornithine decarboxylase 
      (ODC) pathway. SMMC-7721 cells were treated with sodium nitrite, ammonium 
      chloride, sodium nitrite and ammonium chloride mixture for 24 h, the cell 
      viability was analyzed using the MTT assay, cell invasion was analyzed with the 
      transwell assay, the intracellular ROS levels were detected with a reactive 
      oxygen species (ROS) test kits, the expression of intracellular ODC was examined 
      with immunofluorescence and Western blot, the expression of matrix 
      metallopeptidase-2 (MMP-2) and MMP-9 were analyzed by Western blot. Compared with 
      the control group, SMMC-7721 cells exhibited an increase in cell viability, 
      invasion ability, ROS levels and ODC protein after exposure to 150 mumol.L(-1) 
      sodium nitrite and ammonium chloride mixture for 24 h. The invasive activity was 
      reduced by ROS scavenger N-acetycysteine (NAC) in SMMC-7721 cells. The specific 
      ODC inhibitor difluoromethylornithine (DFMO) increased ROS levels and weakened 
      the ability of sodium nitrite and ammonium chloride mixture in the regulation of 
      invasion of SMMC-7721 cells. These data demonstrated that sodium nitrite and 
      ammonium chloride mixture promote invasion of SMMC-7721 cells by enhancing 
      ROS/ODC pathway.
FAU - Meng, Shan-shan
AU  - Meng SS
FAU - Gui, Guan
AU  - Gui G
FAU - Li, Lu-juan
AU  - Li LJ
FAU - Liu, Bin
AU  - Liu B
FAU - Liang, Hong-xia
AU  - Liang HX
FAU - Liu, Liang-ce
AU  - Liu LC
FAU - Huangfu, Chao-shen
AU  - Huangfu CS
LA  - chi
PT  - Journal Article
PL  - China
TA  - Yao Xue Xue Bao
JT  - Yao xue xue bao = Acta pharmaceutica Sinica
JID - 21710340R
RN  - 0 (Reactive Oxygen Species)
RN  - 7664-41-7 (Ammonia)
RN  - EC 3.4.24.24 (MMP2 protein, human)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (MMP9 protein, human)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - EC 4.1.1.17 (Ornithine Decarboxylase)
RN  - M0KG633D4F (Sodium Nitrite)
SB  - IM
MH  - Ammonia/*pharmacology
MH  - Carcinoma, Hepatocellular/*pathology
MH  - Cell Line, Tumor
MH  - Cell Survival
MH  - Humans
MH  - Liver Neoplasms/*pathology
MH  - Matrix Metalloproteinase 2/metabolism
MH  - Matrix Metalloproteinase 9/metabolism
MH  - *Neoplasm Invasiveness
MH  - Ornithine Decarboxylase/*metabolism
MH  - Reactive Oxygen Species/*metabolism
MH  - Sodium Nitrite/*pharmacology
EDAT- 2016/07/01 00:00
MHDA- 2018/08/21 06:00
CRDT- 2018/06/14 06:00
PHST- 2018/06/14 06:00 [entrez]
PHST- 2016/07/01 00:00 [pubmed]
PHST- 2018/08/21 06:00 [medline]
PST - ppublish
SO  - Yao Xue Xue Bao. 2016 Jul;51(7):1083-90.