PMID- 29897615 OWN - NLM STAT- Publisher LR - 20240227 IS - 1522-2683 (Electronic) IS - 0173-0835 (Linking) DP - 2018 Jun 13 TI - Comparison of selected clean and green extraction technologies for biomolecules from apple pomace. LID - 10.1002/elps.201800041 [doi] AB - Apple pomace has been considered as a sustainable source for antioxidant phenolic compounds. Previous reports show extraction of total phenolic contents (TPC) by following various conventional and non-conventional techniques; however, a comparative study has not been reported. In the present work, conventional extraction was compared with several non-conventional extraction methods including ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE), high-speed homogenization (HH). Moreover, efficacy of combined treatments, including high-speed homogenization coupled with MAE and ultrasound-assisted enzymatic extraction (UAEE) was evaluated for the recovery of TPC. The results revealed that UAEE results in the highest TPC (4.62 mg GAE/g), moreover, it simultaneously enabled the recovery of low methoxy pectins with the degree of methylation ranging from 14.03- 28.85%. The LC-Q-Tof analysis revealed the presence of various phenolic acids and flavonoids. The DPPH radical scavenging assay showed that the phenolic rich extracts had IC50 values ranging from 27.1 to 54.6, depending on the extraction parameters. This article is protected by copyright. All rights reserved. CI - This article is protected by copyright. All rights reserved. FAU - Zhang, Zhihang AU - Zhang Z AD - Food Chemistry and Technology, Teagasc Food Research Centre, Ashtown, Dublin, Ireland. FAU - Manjunatha Poojary, Mahesha AU - Manjunatha Poojary M AD - Department of Food Science, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark. FAU - Choudhary, Alka AU - Choudhary A AD - Food Biosciences, Teagasc Food Research Centre, Ashtown, Dublin, Ireland. FAU - Rai, Dilip K AU - Rai DK AD - Food Biosciences, Teagasc Food Research Centre, Ashtown, Dublin, Ireland. FAU - Tiwari, Brijesh K AU - Tiwari BK AD - Food Chemistry and Technology, Teagasc Food Research Centre, Ashtown, Dublin, Ireland. LA - eng PT - Journal Article DEP - 20180613 PL - Germany TA - Electrophoresis JT - Electrophoresis JID - 8204476 OTO - NOTNLM OT - Bioactives OT - Enzyme assisted extraction OT - Phenolic profile OT - Phenolics OT - Ultrasound assisted extraction EDAT- 2018/06/14 06:00 MHDA- 2018/06/14 06:00 CRDT- 2018/06/14 06:00 PHST- 2018/01/29 00:00 [received] PHST- 2018/05/14 00:00 [revised] PHST- 2018/05/14 00:00 [accepted] PHST- 2018/06/14 06:00 [entrez] PHST- 2018/06/14 06:00 [pubmed] PHST- 2018/06/14 06:00 [medline] AID - 10.1002/elps.201800041 [doi] PST - aheadofprint SO - Electrophoresis. 2018 Jun 13. doi: 10.1002/elps.201800041.