PMID- 29899006
OWN - NLM
STAT- MEDLINE
DCOM- 20190626
LR  - 20240214
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 56
IP  - 9
DP  - 2018 Sep
TI  - Fluorescence In Situ Hybridization (FISH) and Peptide Nucleic Acid Probe-Based 
      FISH for Diagnosis of Q Fever Endocarditis and Vascular Infections.
LID - 10.1128/JCM.00542-18 [doi]
LID - e00542-18
AB  - Endocarditis and vascular infections are common manifestations of persistent 
      localized infection due to Coxiella burnetii, and recently, fluorescence in situ 
      hybridization (FISH) was proposed as an alternative tool for their diagnosis. In 
      this study, we evaluated the efficiency of FISH in a series of valve and vascular 
      samples infected by C. burnetii We tested 23 C. burnetii-positive valves and 
      thrombus samples obtained from patients with Q fever endocarditis. Seven 
      aneurysms and thrombus specimens were retrieved from patients with Q fever 
      vascular infections. Samples were analyzed by culture, immunochemistry, and FISH 
      with oligonucleotide and PNA probes targeting C. burnetii-specific 16S rRNA 
      sequences. The immunohistochemical analysis was positive for five (17%) samples 
      with significantly more copies of C. burnetii DNA than the negative ones (P = 
      0.02). FISH was positive for 13 (43%) samples and presented 43% and 40% 
      sensitivity compared to that for quantitative PCR (qPCR) and culture, 
      respectively. PNA FISH detected C. burnetii in 18 (60%) samples and presented 60% 
      and 55% sensitivity compared to that for qPCR and culture, respectively. 
      Immunohistochemistry had 38% and 28% sensitivity compared to that for FISH and 
      PNA FISH, respectively. Samples found positive by both immunohistochemistry and 
      PNA FISH contained significantly more copies of C. burnetii DNA than the negative 
      ones (P = 0.03). Finally, PNA FISH was more sensitive than FISH (60% versus 43%, 
      respectively) for the detection of C. burnetii We provide evidence that PNA FISH 
      and FISH are important assays for the diagnosis of C. burnetii endocarditis and 
      vascular infections.
CI  - Copyright (c) 2018 American Society for Microbiology.
FAU - Prudent, Elsa
AU  - Prudent E
AD  - Aix Marseille Universite, IRD, AP-HM, MEPHI, IHU-Mediterranee Infection, 
      Marseille, France.
FAU - Lepidi, Hubert
AU  - Lepidi H
AD  - Aix Marseille Universite, IRD, AP-HM, MEPHI, IHU-Mediterranee Infection, 
      Marseille, France.
FAU - Angelakis, Emmanouil
AU  - Angelakis E
AD  - Aix Marseille Universite, IRD, AP-HM, VITROME, IHU-Mediterranee Infection, 
      Marseille, France.
AD  - French Reference Center for the Diagnosis and Study of Rickettsioses, Q fever and 
      Bartonelloses, IHU-Mediterranee Infection, Marseille, France.
FAU - Raoult, Didier
AU  - Raoult D
AD  - Aix Marseille Universite, IRD, AP-HM, MEPHI, IHU-Mediterranee Infection, 
      Marseille, France didier.raoult@gmail.com.
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Retracted Publication
DEP - 20180827
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Peptide Nucleic Acids)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
ECI - J Clin Microbiol. 2022 Sep 7;:e0107722. PMID: 36069599
RIN - J Clin Microbiol. 2024 Feb 14;62(2):e0150823. PMID: 38174947
MH  - Coxiella burnetii/genetics
MH  - Endocarditis, Bacterial/*diagnosis/microbiology/pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - *In Situ Hybridization, Fluorescence
MH  - *Molecular Diagnostic Techniques
MH  - Oligonucleotide Probes
MH  - Peptide Nucleic Acids/genetics
MH  - Polymerase Chain Reaction
MH  - Q Fever/*diagnosis/microbiology/pathology
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sensitivity and Specificity
MH  - Vascular Diseases/*diagnosis/microbiology/pathology
PMC - PMC6113452
OTO - NOTNLM
OT  - Coxiella burnetii
OT  - FISH
OT  - PNA probe
OT  - Q fever
OT  - infective endocarditis
OT  - oligonucleotide probe
OT  - vascular infections
EDAT- 2018/06/15 06:00
MHDA- 2019/06/27 06:00
PMCR- 2019/02/27
CRDT- 2018/06/15 06:00
PHST- 2018/03/31 00:00 [received]
PHST- 2018/06/11 00:00 [accepted]
PHST- 2018/06/15 06:00 [pubmed]
PHST- 2019/06/27 06:00 [medline]
PHST- 2018/06/15 06:00 [entrez]
PHST- 2019/02/27 00:00 [pmc-release]
AID - JCM.00542-18 [pii]
AID - 00542-18 [pii]
AID - 10.1128/JCM.00542-18 [doi]
PST - epublish
SO  - J Clin Microbiol. 2018 Aug 27;56(9):e00542-18. doi: 10.1128/JCM.00542-18. Print 
      2018 Sep.