PMID- 29899552
OWN - NLM
STAT- MEDLINE
DCOM- 20191021
LR  - 20191022
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Jun 13
TI  - The Radiated Deep-frozen Xenogenic Meniscal Tissue Regenerated the Total Meniscus 
      with Chondroprotection.
PG  - 9041
LID - 10.1038/s41598-018-27016-w [doi]
LID - 9041
AB  - Meniscal allograft transplantation yields good and excellent results but is 
      limited by donor availability. The purpose of the study was to evaluate the 
      effectiveness of radiated deep-frozen xenogenic meniscal tissue (RDF-X) as an 
      alternative graft choice in meniscal transplantation. The xenogenic meniscal 
      tissues were harvested from the inner 1/3 part of the porcine meniscus and then 
      irradiated and deeply frozen. The medial menisci of rabbits were replaced by the 
      RDF-X. Meniscal allograft transplantation, meniscectomy and sham operation served 
      as controls. Only a particular kind of rabbit-anti-pig antibody (molecular 
      ranging 60-80 kD) was detected in the blood serum at week 2. The menisci of the 
      group RDF-X grossly resembled the native tissue and the allograft meniscus with 
      fibrocartilage regeneration at postoperative 1 year. Cell incorporation and the 
      extracellular matrix were mostly observed at the surface and the inner 1/3 part 
      of the newly regenerated RDF-X, which was different from the allograft. The 
      biomechanical properties of the group RDF-X were also approximate to those of the 
      native meniscus except for the compressive creep. In addition, chondroprotection 
      was achieved after the RDF-X transplantation although the joint degeneration was 
      not completely prevented. To conclude, the RDF-X could be a promising alternative 
      for meniscal transplantation with similar tissue regeneration capacity to 
      allograft transplantation and superior chondroprotection. The potential minor 
      immunological rejection should be further studied before its clinical 
      application.
FAU - Jiang, Dong
AU  - Jiang D
AD  - Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking 
      University Third Hospital, Beijing, 100191, P. R. China.
FAU - Zhang, Zheng-Zheng
AU  - Zhang ZZ
AD  - Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking 
      University Third Hospital, Beijing, 100191, P. R. China.
AD  - Department of Orthopaedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen 
      University, Guangzhou, 510120, P. R. China.
FAU - Zhao, Feng
AU  - Zhao F
AD  - Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, 
      School of Biological Science and Medical Engineering, Beihang University, 
      Beijing, 100191, P. R. China.
FAU - Wang, Shao-Jie
AU  - Wang SJ
AD  - Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking 
      University Third Hospital, Beijing, 100191, P. R. China.
AD  - Department of Joint Surgery, Zhongshan Hospital of Xiamen University, Xiamen 
      University, Xiamen, 361004, P. R. China.
FAU - Qi, Yan-Song
AU  - Qi YS
AD  - Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking 
      University Third Hospital, Beijing, 100191, P. R. China.
FAU - Zhao, Li-Heng
AU  - Zhao LH
AD  - Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking 
      University Third Hospital, Beijing, 100191, P. R. China.
FAU - Zhang, Ji-Ying
AU  - Zhang JY
AD  - Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking 
      University Third Hospital, Beijing, 100191, P. R. China.
FAU - Yu, Jia-Kuo
AU  - Yu JK
AD  - Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking 
      University Third Hospital, Beijing, 100191, P. R. China. yujiakuo@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180613
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - Animals
MH  - Cartilage, Articular/physiopathology/surgery
MH  - Cryopreservation/methods
MH  - Humans
MH  - Menisci, Tibial/physiopathology/*surgery
MH  - Meniscus/*transplantation/ultrastructure
MH  - Microscopy, Electron, Scanning
MH  - Rabbits
MH  - *Regeneration
MH  - Swine
MH  - Synovial Membrane/*physiopathology
MH  - Transplantation, Heterologous
PMC - PMC5998046
COIS- The authors declare no competing interests.
EDAT- 2018/06/15 06:00
MHDA- 2019/10/23 06:00
PMCR- 2018/06/13
CRDT- 2018/06/15 06:00
PHST- 2017/06/14 00:00 [received]
PHST- 2018/05/14 00:00 [accepted]
PHST- 2018/06/15 06:00 [entrez]
PHST- 2018/06/15 06:00 [pubmed]
PHST- 2019/10/23 06:00 [medline]
PHST- 2018/06/13 00:00 [pmc-release]
AID - 10.1038/s41598-018-27016-w [pii]
AID - 27016 [pii]
AID - 10.1038/s41598-018-27016-w [doi]
PST - epublish
SO  - Sci Rep. 2018 Jun 13;8(1):9041. doi: 10.1038/s41598-018-27016-w.