PMID- 29900121
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2212-9685 (Electronic)
IS  - 2212-9685 (Linking)
VI  - 16
DP  - 2017 Sep
TI  - Quantitative proteomic analysis identifies proteins and pathways related to 
      neuronal development in differentiated SH-SY5Y neuroblastoma cells.
PG  - 1-11
LID - 10.1016/j.euprot.2017.06.001 [doi]
AB  - SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic 
      acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of 
      neuronal differentiation. We compared SH-SY5Y cells proteome before and after 
      RA/BDNF treatment using iTRAQ and phosphopeptide enrichment strategies. We 
      identified 5587 proteins, 366 of them with differential abundance. Differentiated 
      cells expressed proteins related to neuronal development, and, undifferentiated 
      cells expressed proteins involved in cell proliferation. Interactive network 
      covered focal adhesion, cytoskeleton dynamics and neurodegenerative diseases 
      processes and regulation of mitogen-activated protein kinase-related signaling 
      pathways; key proteins involved in those processes might be explored as markers 
      for neuronal differentiation.
FAU - Murillo, Jimmy Rodriguez
AU  - Murillo JR
AD  - Proteomics Unit, Chemistry Institute, Federal University of Rio de Janeiro, 
      21941-909, Rio de Janeiro, Brazil.
FAU - Goto-Silva, Livia
AU  - Goto-Silva L
AD  - D'Or Institute for Research and Education (IDOR), 22281-100, Rio de Janeiro, 
      Brazil.
FAU - Sanchez, Aniel
AU  - Sanchez A
AD  - Section for Clinical Chemistry, Department of Translational Medicine, Lund 
      University, Skane University Hospital Malmo, 205 02 Malmo, Sweden.
AD  - Center of Excellence in Biological and Medical Mass Spectrometry, Biomedical 
      Center D13, Lund University, 221 84 Lund, Sweden.
FAU - Nogueira, Fabio C S
AU  - Nogueira FCS
AD  - Proteomics Unit, Chemistry Institute, Federal University of Rio de Janeiro, 
      21941-909, Rio de Janeiro, Brazil.
FAU - Domont, Gilberto B
AU  - Domont GB
AD  - Proteomics Unit, Chemistry Institute, Federal University of Rio de Janeiro, 
      21941-909, Rio de Janeiro, Brazil.
FAU - Junqueira, Magno
AU  - Junqueira M
AD  - Proteomics Unit, Chemistry Institute, Federal University of Rio de Janeiro, 
      21941-909, Rio de Janeiro, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20170623
PL  - Netherlands
TA  - EuPA Open Proteom
JT  - EuPA open proteomics
JID - 101637351
PMC - PMC5965715
OTO - NOTNLM
OT  - Neuronal differentiation
OT  - Phosphoproteomics
OT  - SH-SY5Y cells
OT  - iTRAQ-based proteomics
EDAT- 2018/06/15 06:00
MHDA- 2018/06/15 06:01
PMCR- 2017/06/23
CRDT- 2018/06/15 06:00
PHST- 2016/11/01 00:00 [received]
PHST- 2017/03/07 00:00 [revised]
PHST- 2017/06/21 00:00 [accepted]
PHST- 2018/06/15 06:00 [entrez]
PHST- 2018/06/15 06:00 [pubmed]
PHST- 2018/06/15 06:01 [medline]
PHST- 2017/06/23 00:00 [pmc-release]
AID - S2212-9685(16)30051-4 [pii]
AID - 10.1016/j.euprot.2017.06.001 [doi]
PST - epublish
SO  - EuPA Open Proteom. 2017 Jun 23;16:1-11. doi: 10.1016/j.euprot.2017.06.001. 
      eCollection 2017 Sep.