PMID- 29901388
OWN - NLM
STAT- MEDLINE
DCOM- 20190403
LR  - 20190403
IS  - 1520-4995 (Electronic)
IS  - 0006-2960 (Linking)
VI  - 57
IP  - 28
DP  - 2018 Jul 17
TI  - Response of Heme Symmetry to the Redox State of Bovine Cytochrome c Oxidase.
PG  - 4105-4113
LID - 10.1021/acs.biochem.8b00459 [doi]
AB  - Second-derivative absorption spectroscopy was employed to monitor the response of 
      effective symmetry of cytochromes a and a(3) to the redox and ligation states of 
      bovine cytochrome c oxidase (CcO). The Soret band pi --> pi* electronic transitions 
      were used to display the changes in symmetry of these chromophores induced by the 
      reduction of CcO inhibited by the exogenous ligands and during catalytic 
      turnover. The second derivative of the difference absorption spectra revealed 
      only a single Soret band for the oxidized cytochromes a and a(3) and 
      cyanide-ligated oxidized cytochrome a(3). In contrast, two absorption bands were 
      resolved in ferrous cytochrome a and ferrous cytochrome a(3) ligated with 
      cyanide. A transition from one-band spectrum to two-band spectrum indicates the 
      lowering of symmetry of these hemes due to the alteration of their immediate 
      surroundings. It is suggested that the changes in polarity occurring in the 
      vicinity of these cofactors are main reason for the split of the Soret band of 
      both ferrous cytochrome a and cyanide-bound ferrous cytochrome a(3).
FAU - Kopcova, Katarina
AU  - Kopcova K
AD  - Department of Biophysics, Faculty of Science , University of P. J. Safarik , 
      Jesenna 5 , 041 54 Kosice , Slovak Republic.
FAU - Blascakova, Ludmila
AU  - Blascakova L
AD  - Center for Interdisciplinary Biosciences, Technology and Innovation Park , 
      University of P. J. Safarik , Jesenna 5 , 041 54 Kosice , Slovak Republic.
FAU - Kozar, Tibor
AU  - Kozar T
AD  - Center for Interdisciplinary Biosciences, Technology and Innovation Park , 
      University of P. J. Safarik , Jesenna 5 , 041 54 Kosice , Slovak Republic.
FAU - Jancura, Daniel
AU  - Jancura D
AUID- ORCID: 0000-0003-1968-8709
AD  - Department of Biophysics, Faculty of Science , University of P. J. Safarik , 
      Jesenna 5 , 041 54 Kosice , Slovak Republic.
AD  - Center for Interdisciplinary Biosciences, Technology and Innovation Park , 
      University of P. J. Safarik , Jesenna 5 , 041 54 Kosice , Slovak Republic.
FAU - Fabian, Marian
AU  - Fabian M
AUID- ORCID: 0000-0002-7571-6164
AD  - Center for Interdisciplinary Biosciences, Technology and Innovation Park , 
      University of P. J. Safarik , Jesenna 5 , 041 54 Kosice , Slovak Republic.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180629
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Cyanides)
RN  - 0 (Cytochromes a3)
RN  - 42VZT0U6YR (Heme)
RN  - 9035-34-1 (Cytochromes a)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cyanides/chemistry/metabolism
MH  - Cytochromes a/chemistry/*metabolism
MH  - Cytochromes a3/chemistry/*metabolism
MH  - Electron Transport Complex IV/chemistry/*metabolism
MH  - Electrons
MH  - Heme/chemistry/*metabolism
MH  - Oxidation-Reduction
EDAT- 2018/06/15 06:00
MHDA- 2019/04/04 06:00
CRDT- 2018/06/15 06:00
PHST- 2018/06/15 06:00 [pubmed]
PHST- 2019/04/04 06:00 [medline]
PHST- 2018/06/15 06:00 [entrez]
AID - 10.1021/acs.biochem.8b00459 [doi]
PST - ppublish
SO  - Biochemistry. 2018 Jul 17;57(28):4105-4113. doi: 10.1021/acs.biochem.8b00459. 
      Epub 2018 Jun 29.