PMID- 29901554
OWN - NLM
STAT- MEDLINE
DCOM- 20191111
LR  - 20211204
IS  - 1536-4801 (Electronic)
IS  - 0277-2116 (Linking)
VI  - 67
IP  - 4
DP  - 2018 Oct
TI  - Will the Real Coeliac Disease Please Stand Up? Coeliac Disease Prevalence in the 
      German LIFE Child Study.
PG  - 494-500
LID - 10.1097/MPG.0000000000002052 [doi]
AB  - OBJECTIVES: Assessing the seroprevalence and the prevalence of definite coeliac 
      disease (CD) in the German LIFE Child Health study cohort including 
      immunoglobulin A (IgA) antibodies against tissue transglutaminase (IgA-TTG) in 
      addition to IgG antibodies against deamidated gliadin peptides (IgG-DGP) and 
      human leukocyte antigen (HLA)-DQ2/8 genotyping. METHODS: Samples from children 
      and adolescents were first screened for IgA-TTG and IgG-DGP. If IgA-TTG was above 
      0.5 times the upper limit of normal and/or IgG-DGP was positive, IgA antibodies 
      against endomysium (IgA-EmA) were measured, and HLA was genotyped. In patients 
      with only IgG-DGP positivity, total IgA was assayed. Subjects with suspicious 
      results were followed up serologically and, in case of repeatedly positive 
      antibody results, invited for a personal interview. Further diagnostic data were 
      obtained independent from our study. RESULTS: We screened 2363 children's blood 
      samples collected from 2011 to 2015. The seroprevalence, that is, IgA-TTG and/or 
      IgA-EmA positivity or IgG-DGP positivity with IgA <0.05 g/L, was 1.57% (95% 
      confidence interval [CI95%] 1.14-2.15). The prevalence of suspected CD, that is, 
      seroprevalence and compatible HLA genotype with hitherto unknown mucosal damage, 
      was 1.35% (CI95% 0.96-1.91). Definite CD, that is, seropositivity accompanied by 
      positive intestinal biopsy or IgA-TTG >/= 10 x upper limit of normal, was found in 
      0.42% (CI95% 0.22-0.80). Seven children claimed to have CD. The HLA haplotype, 
      however, matched in only 4 of them resulting in an overall CD prevalence of at 
      least 0.59% (CI95% 0.34-1.02). Thirteen unclear cases remained; therefore, the 
      prevalence may even be higher. CONCLUSIONS: The prevalence of definite CD in a 
      population-representative German cohort is higher than previously described. 
      HLA-DQ typing is helpful to identify false-positive IgA-TTG patients negative for 
      IgA-EmA and/or IgG-DGP under screening conditions and unmasks possible 
      misdiagnoses of CD.
FAU - Handel, Norman
AU  - Handel N
AD  - Department of Women and Child Health, University Hospital for Children and 
      Adolescents and Center for Pediatric Research.
FAU - Mothes, Thomas
AU  - Mothes T
AD  - Institute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics.
FAU - Petroff, David
AU  - Petroff D
AD  - Clinical Trial Centre.
FAU - Baber, Ronny
AU  - Baber R
AD  - Institute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics.
AD  - LIFE Leipzig Research Centre for Civilization Diseases, University of Leipzig, 
      Leipzig.
FAU - Jurkutat, Anne
AU  - Jurkutat A
AD  - LIFE Leipzig Research Centre for Civilization Diseases, University of Leipzig, 
      Leipzig.
FAU - Flemming, Gunter
AU  - Flemming G
AD  - Department of Women and Child Health, University Hospital for Children and 
      Adolescents and Center for Pediatric Research.
FAU - Kiess, Wieland
AU  - Kiess W
AD  - Department of Women and Child Health, University Hospital for Children and 
      Adolescents and Center for Pediatric Research.
AD  - LIFE Leipzig Research Centre for Civilization Diseases, University of Leipzig, 
      Leipzig.
FAU - Hiemisch, Andreas
AU  - Hiemisch A
AD  - Department of Women and Child Health, University Hospital for Children and 
      Adolescents and Center for Pediatric Research.
AD  - LIFE Leipzig Research Centre for Civilization Diseases, University of Leipzig, 
      Leipzig.
FAU - Korner, Antje
AU  - Korner A
AD  - Department of Women and Child Health, University Hospital for Children and 
      Adolescents and Center for Pediatric Research.
AD  - LIFE Leipzig Research Centre for Civilization Diseases, University of Leipzig, 
      Leipzig.
FAU - Schlumberger, Wolfgang
AU  - Schlumberger W
AD  - EUROIMMUN Medizinische Labordiagnostika AG, Lubeck/Dassow, Germany.
FAU - Thiery, Joachim
AU  - Thiery J
AD  - Institute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics.
AD  - LIFE Leipzig Research Centre for Civilization Diseases, University of Leipzig, 
      Leipzig.
FAU - Wolf, Johannes
AU  - Wolf J
AD  - Institute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Pediatr Gastroenterol Nutr
JT  - Journal of pediatric gastroenterology and nutrition
JID - 8211545
RN  - 0 (Autoantibodies)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 9007-90-3 (Gliadin)
RN  - EC 2.3.2.13 (Protein Glutamine gamma Glutamyltransferase 2)
RN  - EC 2.3.2.13 (Transglutaminases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Adolescent
MH  - Autoantibodies/*blood
MH  - Celiac Disease/diagnosis/*epidemiology
MH  - Child
MH  - Diagnostic Errors
MH  - Female
MH  - GTP-Binding Proteins/immunology
MH  - Genotyping Techniques
MH  - Germany/epidemiology
MH  - Gliadin/immunology
MH  - HLA-DQ Antigens
MH  - Humans
MH  - Immunoglobulin A/immunology
MH  - Immunoglobulin G/immunology
MH  - Longitudinal Studies
MH  - Male
MH  - Mass Screening/*statistics & numerical data
MH  - Prevalence
MH  - Prospective Studies
MH  - Protein Glutamine gamma Glutamyltransferase 2
MH  - Seroepidemiologic Studies
MH  - Transglutaminases/immunology
EDAT- 2018/06/15 06:00
MHDA- 2019/11/12 06:00
CRDT- 2018/06/15 06:00
PHST- 2018/06/15 06:00 [pubmed]
PHST- 2019/11/12 06:00 [medline]
PHST- 2018/06/15 06:00 [entrez]
AID - 10.1097/MPG.0000000000002052 [doi]
PST - ppublish
SO  - J Pediatr Gastroenterol Nutr. 2018 Oct;67(4):494-500. doi: 
      10.1097/MPG.0000000000002052.