PMID- 29901584
OWN - NLM
STAT- MEDLINE
DCOM- 20180626
LR  - 20240327
IS  - 1536-5964 (Electronic)
IS  - 0025-7974 (Print)
IS  - 0025-7974 (Linking)
VI  - 97
IP  - 24
DP  - 2018 Jun
TI  - The association between MCP-1, VEGF polymorphisms and their serum levels in 
      patients with diabetic foot ulcer.
PG  - e10959
LID - 10.1097/MD.0000000000010959 [doi]
LID - e10959
AB  - The purpose of the present study was to investigate distribution of monocyte 
      chemoattractant protein-1 (MCP-1) -2518A/G and vascular endothelial growth factor 
      (VEGF) -634G/C polymorphisms in type 2 diabetes melitus patients (T2DM) 
      presenting diabetic foot ulcer (DFU). Additionally, we evaluated the effects of 
      these 2 polymorphisms on serum levels of MCP-1 and VEGF in the study 
      population.Patients diagnosed with T2DM without or with DFU were recruited in the 
      study. The distribution of MCP-1 -2518A/G and VEGF -634G/C polymorphisms was 
      investigated by polymerase chain reaction-restriction fragment length 
      polymorphism (PCR-RFLP). Enzyme-linked immunosorbent assay (ELISA) was applied to 
      detect the protein levels of MCP-1 and VEGF. The comparisons of protein levels in 
      DFU patients were performed by student t test according to their genotypes.The 
      frequencies of GG genotype and G allele of MCP-1 -2518A/G was increased in DFU 
      patients, compared with T2DM patients (odds ratio [OR] = 2.60, 95% confidence 
      interval [CI] = 1.23-5.50, P = .011 and OR = 1.72, 95% CI = 1.18-2.50, P = .005, 
      respectively). Moreover, the increased frequency of GG was significantly 
      associated with up-regulated MCP-1 level in DFU patients (P < .001). Analysis for 
      VEGF -634G/C polymorphisms indicated that the prevalence of CC genotype and C 
      allele of the polymorphisms was decreased in DFU patients, compared with T2DM 
      patients (OR = 0.36, 95% CI = 0.17-0.77, P = .008 and OR = 0.63, 95% 
      CI = 0.43-0.91, P = .015, respectively). DFU patients carrying CC genotype had a 
      higher level of VEGF than those with other genotypes (P = .007).MCP-1 -2518A/G 
      and VEGF -634G/C polymorphisms may involve in occurrence and progress of DFU 
      through regulating transcription activity of the genes.
FAU - Li, Xiaolei
AU  - Li X
AD  - First Department of Orthopedics, The First Affiliated Hospital of Shihezi 
      University Medical College, Shihezi, Xinjiang, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Observational Study
PL  - United States
TA  - Medicine (Baltimore)
JT  - Medicine
JID - 2985248R
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Asian People/genetics
MH  - Chemokine CCL2/blood/*genetics
MH  - Diabetes Mellitus, Type 2/blood/*genetics
MH  - Diabetic Foot/blood/*genetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Genetic
MH  - Polymorphism, Restriction Fragment Length
MH  - Vascular Endothelial Growth Factor A/blood/*genetics
PMC - PMC6024659
COIS- The authors declare that they have no conflict of interest.
EDAT- 2018/06/15 06:00
MHDA- 2018/06/27 06:00
PMCR- 2018/06/15
CRDT- 2018/06/15 06:00
PHST- 2018/06/15 06:00 [entrez]
PHST- 2018/06/15 06:00 [pubmed]
PHST- 2018/06/27 06:00 [medline]
PHST- 2018/06/15 00:00 [pmc-release]
AID - 00005792-201806150-00012 [pii]
AID - MD-D-17-07522 [pii]
AID - 10.1097/MD.0000000000010959 [doi]
PST - ppublish
SO  - Medicine (Baltimore). 2018 Jun;97(24):e10959. doi: 10.1097/MD.0000000000010959.