PMID- 2990168
OWN - NLM
STAT- MEDLINE
DCOM- 19850731
LR  - 20131121
IS  - 0001-6772 (Print)
IS  - 0001-6772 (Linking)
VI  - 124
IP  - 2
DP  - 1985 Jun
TI  - Comparison between the steroidogenic responses after pulsatile and continuous 
      administration of gonadotrophin releasing hormone to superfused rat follicles.
PG  - 269-76
AB  - Gonadotrophin releasing hormone (GnRH) exhibits both stimulatory and inhibitory 
      effects on the ovarian tissue in the rat. For the action of GnRH on the 
      pituitary, the mode of administration is of utmost importance. The binding 
      characteristics and regulation of the GnRH receptors found in the ovary are very 
      similar to those of the pituitary. The aim of the present study was to compare 
      the steroidogenic response of superfused, preovulatory rat follicles to 
      continuous and pulsatile administration of a GnRH analogue (GnRHa). The results 
      obtained with a single pulse of luteinizing hormone (LH) served as a control. 
      Repeated pulses (1 pulse X h-1) or continuous administration of GnRHa was found 
      to be more effective than a single pulse of GnRHa. The effect of GnRHA, 
      irrespective of the mode of administration, was most pronounced on the secretion 
      of 20 alpha-OH-progesterone (20 alpha-OHP). Also the release of testosterone (T) 
      and oestradiol (E2) was stimulated but to a lesser extent. In comparison to LH, 
      the GnRHa response was delayed and without detectable release of cyclic AMP 
      (cAMP). These results illustrate that pulses of GnRH stimulate the 
      steroidogenesis in preovulatory follicles. In contrast to the effects of GnRHa on 
      the pituitary, the modes of administration seem to be of less importance for the 
      stimulation of the rat ovary.
FAU - Hedin, L
AU  - Hedin L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Acta Physiol Scand
JT  - Acta physiologica Scandinavica
JID - 0370362
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, LHRH)
RN  - 145-14-2 (20-alpha-Dihydroprogesterone)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 3XMK78S47O (Testosterone)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 52435-06-0 (surfagon)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - E0399OZS9N (Cyclic AMP)
SB  - IM
MH  - 20-alpha-Dihydroprogesterone/metabolism
MH  - Animals
MH  - Cyclic AMP/metabolism
MH  - Estradiol/metabolism
MH  - Female
MH  - Gonadal Steroid Hormones/*metabolism
MH  - Gonadotropin-Releasing Hormone/administration & dosage/*analogs & derivatives
MH  - Luteinizing Hormone/pharmacology
MH  - Ovarian Follicle/*metabolism
MH  - Perfusion
MH  - Progesterone/metabolism
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Receptors, Cell Surface/metabolism
MH  - Receptors, LHRH
MH  - Testosterone/metabolism
EDAT- 1985/06/01 00:00
MHDA- 1985/06/01 00:01
CRDT- 1985/06/01 00:00
PHST- 1985/06/01 00:00 [pubmed]
PHST- 1985/06/01 00:01 [medline]
PHST- 1985/06/01 00:00 [entrez]
AID - 10.1111/j.1748-1716.1985.tb07661.x [doi]
PST - ppublish
SO  - Acta Physiol Scand. 1985 Jun;124(2):269-76. doi: 
      10.1111/j.1748-1716.1985.tb07661.x.