PMID- 29902635
OWN - NLM
STAT- MEDLINE
DCOM- 20190204
LR  - 20190215
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 117
DP  - 2018 Oct 15
TI  - Overcoming non-specific binding to measure the active concentration and kinetics 
      of serum anti-HLA antibodies by surface plasmon resonance.
PG  - 191-200
LID - S0956-5663(18)30443-3 [pii]
LID - 10.1016/j.bios.2018.06.013 [doi]
AB  - Human leukocyte antigen (HLA) donor-specific antibodies are key serum biomarkers 
      for assessing the outcome of transplanted patients. Measuring their active 
      concentration, i.e. the fraction that really interacts with donor HLA, and their 
      affinity could help deciphering their pathogenicity. Surface plasmon resonance 
      (SPR) is recognized as the gold-standard for measuring binding kinetics but also 
      active concentrations, without calibration curves. SPR-based biosensors often 
      suffer from non-specific binding (NSB) occurring with the sensor chip surface and 
      the immobilized targets, especially for complex media such as human serum. In 
      this work we show that several serum treatments such as dialysis or IgG 
      purification reduce NSB but insufficiently for SPR applications. We then 
      demonstrate that the NSB contribution to the SPR signal can be eliminated to 
      determine precisely and reliably the active concentration and the affinity of 
      anti-HLA antibodies from patients' sera. This was achieved even at concentrations 
      close to the limit of quantification of the method, in the 0.5-1 nM range. The 
      robustness of the assay was demonstrated by using a wide range of artificially 
      generated NSB and by varying the density of the targets captured onto the 
      surface. The assay is of general interest and can be used with molecules 
      generating strong NSB, as far as a non-cognate target structurally close to the 
      target can be captured on the same flow cell, in a different binding cycle. 
      Compared with current fluorescence-based methods that are semi-quantitative, we 
      expect this SPR-based assay to help better understanding anti-HLA antibodies 
      pathogenicity and improving organ recipients' management.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Visentin, Jonathan
AU  - Visentin J
AD  - CHU de Bordeaux, Laboratoire d'Immunologie et Immunogenetique, Hopital Pellegrin, 
      Place Amelie Raba Leon, Bordeaux, France; Immuno ConcEpT, UMR CNRS 5164, 146 rue 
      Leo Saignat, Bordeaux, France; Universite de Bordeaux, 146 rue Leo Saignat, 
      Bordeaux, France. Electronic address: jonathan.visentin@chu-bordeaux.fr.
FAU - Couzi, Lionel
AU  - Couzi L
AD  - Immuno ConcEpT, UMR CNRS 5164, 146 rue Leo Saignat, Bordeaux, France; Universite 
      de Bordeaux, 146 rue Leo Saignat, Bordeaux, France; CHU de Bordeaux, Service de 
      Nephrologie-Transplantation-Dialyse-Apherese, Hopital Pellegrin, Place Amelie 
      Raba Leon, Bordeaux, France.
FAU - Dromer, Claire
AU  - Dromer C
AD  - CHU de Bordeaux, Service des Maladies Respiratoires, Hopital Haut-Leveque, Avenue 
      de Magellan, Pessac, France.
FAU - Neau-Cransac, Martine
AU  - Neau-Cransac M
AD  - CHU de Bordeaux, Service de Chirurgie digestive et endocrinienne, Hopital 
      Haut-Leveque, Avenue de Magellan, Pessac, France.
FAU - Guidicelli, Gwendaline
AU  - Guidicelli G
AD  - CHU de Bordeaux, Laboratoire d'Immunologie et Immunogenetique, Hopital Pellegrin, 
      Place Amelie Raba Leon, Bordeaux, France.
FAU - Veniard, Vincent
AU  - Veniard V
AD  - CHU de Bordeaux, Service de Chirurgie cardiaque, Hopital Haut-Leveque, Avenue de 
      Magellan, Pessac, France.
FAU - Coniat, Karine Nubret-le
AU  - Coniat KN
AD  - CHU de Bordeaux, Service de Chirurgie cardiaque, Hopital Haut-Leveque, Avenue de 
      Magellan, Pessac, France.
FAU - Merville, Pierre
AU  - Merville P
AD  - Immuno ConcEpT, UMR CNRS 5164, 146 rue Leo Saignat, Bordeaux, France; Universite 
      de Bordeaux, 146 rue Leo Saignat, Bordeaux, France; CHU de Bordeaux, Service de 
      Nephrologie-Transplantation-Dialyse-Apherese, Hopital Pellegrin, Place Amelie 
      Raba Leon, Bordeaux, France.
FAU - Di Primo, Carmelo
AU  - Di Primo C
AD  - Universite de Bordeaux, Laboratoire ARNA, 146 rue Leo Saignat, Bordeaux, France; 
      INSERM U1212, CNRS UMR 5320, IECB, 2 rue Robert Escarpit, Pessac, France. 
      Electronic address: carmelo.diprimo@inserm.fr.
FAU - Taupin, Jean-Luc
AU  - Taupin JL
AD  - CHU de Bordeaux, Laboratoire d'Immunologie et Immunogenetique, Hopital Pellegrin, 
      Place Amelie Raba Leon, Bordeaux, France; Immuno ConcEpT, UMR CNRS 5164, 146 rue 
      Leo Saignat, Bordeaux, France; Universite de Bordeaux, 146 rue Leo Saignat, 
      Bordeaux, France. Electronic address: jean-luc.taupin@aphp.fr.
LA  - eng
PT  - Journal Article
DEP - 20180607
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Antibodies)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Antibodies/*analysis/*metabolism
MH  - Biosensing Techniques/*instrumentation/*methods
MH  - Histocompatibility Antigens Class I/immunology
MH  - Humans
MH  - Kinetics
MH  - Limit of Detection
MH  - Oligonucleotide Array Sequence Analysis
MH  - *Surface Plasmon Resonance
OTO - NOTNLM
OT  - Active concentration
OT  - Anti-HLA antibodies
OT  - Capture
OT  - Kinetics
OT  - Non specific binding
OT  - Surface plasmon resonance
EDAT- 2018/06/15 06:00
MHDA- 2019/02/05 06:00
CRDT- 2018/06/15 06:00
PHST- 2018/03/28 00:00 [received]
PHST- 2018/06/04 00:00 [revised]
PHST- 2018/06/05 00:00 [accepted]
PHST- 2018/06/15 06:00 [pubmed]
PHST- 2019/02/05 06:00 [medline]
PHST- 2018/06/15 06:00 [entrez]
AID - S0956-5663(18)30443-3 [pii]
AID - 10.1016/j.bios.2018.06.013 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2018 Oct 15;117:191-200. doi: 10.1016/j.bios.2018.06.013. 
      Epub 2018 Jun 7.