PMID- 29903113
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 46
IP  - 2
DP  - 2017 Mar
TI  - [Detection method of phthalate esters and their metabolites in blood].
PG  - 309-317
AB  - OBJECTIVE: A method for determination of phthalate esters and their metabolites 
      in blood by using SPE-ultra performance liquid chromatography tandem mass 
      spectrometry. METHODS: A stable isotope detection method been developed for the 
      determination of 20 kinds of phthalate esters and 6 kinds of phthalate esters 
      metabolites in blood by ultra-performance liquid chromatography-mass 
      spectrometry( UPLC-MS) coupled with SPE column purification. Blood samples were 
      diluted with acetate buffer( pH5. 2), enzymatic hydrolyzed by beta-glucuronidase for 
      12 h, then purified with HLB column. An ACQUITYBEH Phenyl column( 2. 1 mm x100 
      mm, 1. 7 mum) was used for separation by the gradient elution with acetonitrile 
      and aqueous solution containing 0. 02% formic acid as the mobile phases. In the 
      present study, an electrospray ionization( ESI) source was used, and the internal 
      standard method was used for quantitation. RESULTS: The linear ranges of the 26 
      analytes were from 1. 0- 20. 0 mug/L, the coefficients of correlation were greater 
      than 0. 995. The limits of detection( LODs) of the 26 analytes were all lower 
      than1. 0 mug/L. Recoveries studies were carried out using serum samples fortified 
      with the 26 analytes at the levels of 2. 5, 5. 0 and 10. 0 mug/L, recoveries were 
      obtained in the range of63. 0%- 97. 7% with relative standard deviations( RSDs) 
      from 1. 9% to 19. 1%. CONCLUSION: The established method is accurate and highly 
      sensitive, and can be used for the qualitative and quantitative analysis of the 
      residues of the phthalate esters and their metabolites in the blood samples.
FAU - Fan, Sai
AU  - Fan S
AD  - Beijing Centre for Disease Control and Prevention, Beijing 100013, China.
FAU - Zou, Jianhong
AU  - Zou J
AD  - Beijing Centre for Disease Control and Prevention, Beijing 100013, China.
FAU - Xue, Ying
AU  - Xue Y
AD  - Beijing Centre for Disease Control and Prevention, Beijing 100013, China.
FAU - Liu, Ping
AU  - Liu P
AD  - Beijing Centre for Disease Control and Prevention, Beijing 100013, China.
FAU - Wu, Guohua
AU  - Wu G
AD  - Beijing Centre for Disease Control and Prevention, Beijing 100013, China.
FAU - Zhao, Rong
AU  - Zhao R
AD  - Beijing Centre for Disease Control and Prevention, Beijing 100013, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 0 (Esters)
RN  - 0 (Phthalic Acids)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Esters/*blood
MH  - Phthalic Acids/*blood
MH  - Solid Phase Extraction
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - UPLC-MS
OT  - blood samples
OT  - phthalate esters and their metabolites
OT  - solid phase extraction
EDAT- 2017/03/01 00:00
MHDA- 2018/12/12 06:00
CRDT- 2018/06/16 06:00
PHST- 2018/06/16 06:00 [entrez]
PHST- 2017/03/01 00:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2017 Mar;46(2):309-317.