PMID- 29903188
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 46
IP  - 4
DP  - 2017 Jul
TI  - [Determination of 8 components in healthy food for anti-hangover and 
      hepatoprotection by high performance liquid chromatography].
PG  - 633-639
AB  - OBJECTIVE: To develop a simple and sensitive high performance liquid 
      chromatographic method for simultaneous determination of catechin hydrate, 
      epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate, 
      dihydromyricetin, glycyrrhizic acid and glycyrrhetinic acid in healthy food for 
      anti-hangover and hepatoprotection, and compare with the capillary 
      electrophoresis method established by our laboratory. METHODS: The samples were 
      ultrasonically extracted by using methanol-water( 4ratio 1, V/V) for 30 minutes and 
      then centrifuged at 10 000 r/min for 10 minutes. The supernatant was filtered and 
      injected into the HPLC system and then separated on a C_(18) column( 5 mum x 250 
      mm x 4. 6 mm) at 30℃ with gradient elution at a flow rate of 0. 8mL/min. 
      Catechins and dihydromyricetin were detected at the wavelength of 210 nm, 
      glycyrrhizic acid and glycyrrhetinic acid were detected at 250 nm. RESULTS: Under 
      the optimal analytical conditions, the peak area of each analyte and its 
      concentration had agood correlation within the linear range( r >/= 0. 9996). The 
      limits of detection and quantification of the method were 0. 07-1. 25 mug/g( S/N = 
      3) and 0. 22-4. 18 mug/g( S/N = 10), respectively. The intra-and inter-day 
      relative standard deviations( RSDs)of the mixed standard solution were 0. 26%-1. 
      95% and 1. 17%-3. 89%, respectively. The spiked recoveries of the analytes were 
      86. 15%-98. 61%. CONCLUSION: The established method is sensitive and reliable, 
      and could be used for quality control of the healthy food for anti-hangover and 
      hepatoprotection.
FAU - Zou, Haimin
AU  - Zou H
AD  - Department of Laboratory Science in Public Health, West China School of Public 
      Health, Sichuan University, Chengdu 610041, China.
FAU - Zhou, Chen
AU  - Zhou C
AD  - Department of Laboratory Science in Public Health, West China School of Public 
      Health, Sichuan University, Chengdu 610041, China.
FAU - Sun, Chengjun
AU  - Sun C
AD  - Department of Laboratory Science in Public Health, West China School of Public 
      Health, Sichuan University, Chengdu 610041, China.
FAU - Yang, Xiaosong
AU  - Yang X
AD  - Department of Laboratory Science in Public Health, West China School of Public 
      Health, Sichuan University, Chengdu 610041, China.
FAU - Wen, Jun
AU  - Wen J
AD  - Department of Laboratory Science in Public Health, West China School of Public 
      Health, Sichuan University, Chengdu 610041, China.
FAU - Li, Yongxin
AU  - Li Y
AD  - Department of Laboratory Science in Public Health, West China School of Public 
      Health, Sichuan University, Chengdu 610041, China.
FAU - Zeng, Hongyan
AU  - Zeng H
AD  - Department of Laboratory Science in Public Health, West China School of Public 
      Health, Sichuan University, Chengdu 610041, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 0 (Flavonols)
RN  - 6FO62043WK (Glycyrrhizic Acid)
RN  - 8R1V1STN48 (Catechin)
RN  - 92587OVD8Z (epicatechin gallate)
RN  - BQM438CTEL (epigallocatechin gallate)
RN  - HEJ6575V1X (gallocatechol)
RN  - KD8QND6427 (dihydromyricetin)
RN  - P540XA09DR (Glycyrrhetinic Acid)
SB  - IM
MH  - Catechin/analogs & derivatives/*analysis/isolation & purification
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Electrophoresis, Capillary
MH  - Flavonols/*analysis/isolation & purification
MH  - Glycyrrhetinic Acid/*analysis/isolation & purification
MH  - Glycyrrhizic Acid/*analysis/isolation & purification
MH  - Humans
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - catechins
OT  - dihydromyricetin
OT  - glycyrrhetinic acid
OT  - glycyrrhizic acid
OT  - high performance liquid chromatography
EDAT- 2018/06/16 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/06/16 06:00
PHST- 2018/06/16 06:00 [entrez]
PHST- 2018/06/16 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2017 Jul;46(4):633-639.