PMID- 29903308
OWN - NLM
STAT- MEDLINE
DCOM- 20181106
LR  - 20181106
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 46
IP  - 5
DP  - 2017 Sep
TI  - [Determination of 4 kinds of plant growth regulator in bean sprout by solid phase 
      extraction column coupled with ultra-high performance liquid chromatography].
PG  - 783-812
AB  - OBJECTIVE: A method for the determination of 6-benzylaminopurine( 6-BAP), 
      isopentennyladenine( z-IP), 4-fluorophenoxyacetic acid( 4-FPA), 
      4-chlorophenoxyacetic acid( 4-CPA) in bean sprout was developed using solid phase 
      extraction column with ultra-high performance liquid chromatography. METHODS: The 
      sample was extracted by acetonitrile,dehydrated by salt,then centrifugation,and 
      purified by PXC/PWA solid phase extraction column. The chromatographic analysis 
      was carried out on C18 chromatographic column( 100 mm x2. 1 mm,1. 8 
      mum),acetonitrile and sodium dihydrogen phosphate for gradient elution,diode array 
      detector for detection,and quantified with external standard method. RESULTS: The 
      calibration curves showed good linearity in the range of 0. 25-25 mug/mL( 6-BAP 
      and z-IP) and 0. 50-50 mug/mL( 4-FPA and 4-CPA) with correlation coefficients 
      greater than 0. 999. Three levels spiked recoveries were carried out using blank 
      bean sprout extraction as substrate,the recoveries ranged from70. 0% to 96. 
      4%,and the relative standard deviations( RSDs) ranged from 2. 84% to12. 10%( n = 
      6). The qualitative limits of detections were 0. 0082-0. 075 mg/kg and the 
      quantitative limits were 0. 027-0. 25 mg/kg for the 4 PGRs. CONCLUSION: The 
      method is simple and easy to operate using solid phase extraction column 
      coupled,simultaneous determination of 4 PGRs by ultra-high performance liquid 
      chromatography,can ensure the corresponding accuracy,sensitivity and precision.
FAU - Yuan, Wenxin
AU  - Yuan W
AD  - Pinggu District Center for Disease Control and Prevention, Beijing 101200, China.
FAU - Liu, Zhiping
AU  - Liu Z
AD  - Pinggu District Center for Disease Control and Prevention, Beijing 101200, China.
FAU - Liu, Ping
AU  - Liu P
FAU - Zhao, Rong
AU  - Zhao R
AD  - Pinggu District Center for Disease Control and Prevention, Beijing 101200, China.
FAU - Wu, Guohua
AU  - Wu G
AD  - Pinggu District Center for Disease Control and Prevention, Beijing 101200, China.
FAU - Fan, Sai
AU  - Fan S
AD  - Pinggu District Center for Disease Control and Prevention, Beijing 101200, China.
FAU - Zhou, Yang
AU  - Zhou Y
AD  - Pinggu District Center for Disease Control and Prevention, Beijing 101200, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 0 (Benzyl Compounds)
RN  - 0 (Pentanols)
RN  - 0 (Plant Growth Regulators)
RN  - 0 (Purines)
RN  - 2577AQ9262 (2,4-Dichlorophenoxyacetic Acid)
RN  - 27214-40-0 (isopentenol)
RN  - 4EMM3U5P3K (4-chlorophenoxyacetic acid)
RN  - KXG6A989PS (benzylaminopurine)
SB  - IM
MH  - 2,4-Dichlorophenoxyacetic Acid/*analogs & derivatives/*analysis
MH  - Benzyl Compounds/*analysis
MH  - *Chromatography, High Pressure Liquid
MH  - Food Analysis/*methods
MH  - Humans
MH  - Pentanols
MH  - Plant Growth Regulators/*analysis
MH  - Purines/*analysis
MH  - *Solid Phase Extraction
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - bean sprout
OT  - plant growth regulator
OT  - solid phase extraction column
OT  - ultra-high performance liquid chromatography(UPLC)
EDAT- 2018/06/16 06:00
MHDA- 2018/11/07 06:00
CRDT- 2018/06/16 06:00
PHST- 2018/06/16 06:00 [entrez]
PHST- 2018/06/16 06:00 [pubmed]
PHST- 2018/11/07 06:00 [medline]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2017 Sep;46(5):783-812.