PMID- 29904905
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230926
IS  - 2197-1153 (Print)
IS  - 2197-1153 (Electronic)
IS  - 2197-1153 (Linking)
VI  - 5
IP  - 1
DP  - 2018 Jun 15
TI  - MMP-3 in the peripheral serum as a biomarker of knee osteoarthritis, 40 years 
      after open total knee meniscectomy.
PG  - 21
LID - 10.1186/s40634-018-0132-x [doi]
LID - 21
AB  - BACKGROUND: To explore potential biomarkers in a meniscectomy-induced knee 
      osteoarthritis model, at forty years after meniscectomy. METHODS: We carried out 
      a forty-year study of 53 patients who, as adolescents, underwent open total 
      meniscectomy and assessed two potential synovial and serum biomarkers, namely 
      glycosaminoglycan (GAG) and matrix metalloproteinase-3 (MMP-3). Of the 30 
      patients available for review, 8 had contralateral knee operations and were 
      excluded. Of the remaining 22 patients, 17 had successful operated knee synovial 
      fluid aspirations and 8 also had successful contralateral control knee 
      aspirations. GAG and MMP3 levels in the synovial fluid and peripheral serum was 
      measured using Alcian blue precipitation and ELISA quantification, respectively. 
      Patients also had their knee radiographs assessed and their radiographic 
      osteoarthritis classified as per the Kellgren-Lawrence and Ahlbӓck systems. 
      RESULTS: At forty years after meniscectomy, synovial MMP-3 levels remain 
      increased (p = 0.0132) while GAG levels were reduced (p = 0.0487) when compared 
      to controls and these two levels correlate inversely. Furthermore, levels of 
      synovial MMP-3 significantly correlated (p = 0.0032, r = 0.7734; p = 0.0256, 
      r = 0.5552) and GAG levels significantly inversely correlated (p = 0.0308, 
      r = - 0.6220; p = 0.0135, r = - 0.6024), respectively, with both radiological 
      scoring systems. Interestingly, we found that the levels of serum MMP-3 
      correlated only with the synovial fluid levels of MMP-3 in the operated knee and 
      not with the non-operated joint (p = 0.0252, r = 0.7706 vs. p = 0.0764, 
      r = 0.6576). Multiple regression analysis for patient's quality of life based on 
      these biomarkers revealed an almost perfect result with an R2 of 0.9998 and a p 
      value = 0.0087. CONCLUSION: Our results suggest that serum levels of MMP3 could 
      be used as a potential biomarker for knee osteoarthritis, using a simple blood 
      test. Larger cohorts are desirable in order to prove or disprove this finding.
FAU - Pengas, Ioannis
AU  - Pengas I
AUID- ORCID: 0000-0001-9602-9104
AD  - Consultant Trauma & Orthopaedic Knee Surgeon, Joint Preservation & Soft Tissue 
      Knee Specialist, Royal Cornwall Teaching Hospitals NHS Trust Treliske, Truro, TR1 
      3LQ, UK. yiannispengas@yahoo.com.
FAU - Eldridge, Suzanne
AU  - Eldridge S
AD  - Department of Experimental Medicine and Rheumatology, William Harvey Research 
      Institute. Barts and The London, Queen Mary's School of Medicine and Dentistry, 
      London, EC1M 6BQ, UK.
FAU - Assiotis, Aggelos
AU  - Assiotis A
AD  - Specialist registrar in Trauma & Orthopaedics, Chelsea & Westminster Hospital NHS 
      Foundation Trust, 369 Fulham Rd, Chelsea, London, SW10 9NH, UK.
FAU - McNicholas, Michael
AU  - McNicholas M
AD  - Consultant Trauma & Orthopaedic Knee Surgeon, Aintree University Hospital NHS 
      Foundation Trust Longmoor Ln, Liverpool, L9 7AL, UK.
FAU - Mendes, Joao Espregueira
AU  - Mendes JE
AD  - Orthopaedics Department of Minho University, R. da Universidade, Minho 
      University, 4710-057, Braga, Portugal.
FAU - Laver, Lior
AU  - Laver L
AD  - Department of Arthroscopy, The Royal Orthopaedic Hospital, The Woodlands, Bristol 
      Rd S, Birmingham, B31 2AP, UK.
LA  - eng
PT  - Journal Article
DEP - 20180615
PL  - United States
TA  - J Exp Orthop
JT  - Journal of experimental orthopaedics
JID - 101653750
PMC - PMC6003895
COIS- COMPETING INTERESTS: No benefits in any form have been received or will be 
      received from a commercial party related directly or indirectly with the subject 
      of this article. The authors also wish to declare no conflict of interest 
      relevant to this study. PUBLISHER'S NOTE: Springer Nature remains neutral with 
      regard to jurisdictional claims in published maps and institutional affiliations.
EDAT- 2018/06/16 06:00
MHDA- 2018/06/16 06:01
PMCR- 2018/06/15
CRDT- 2018/06/16 06:00
PHST- 2018/03/01 00:00 [received]
PHST- 2018/05/17 00:00 [accepted]
PHST- 2018/06/16 06:00 [entrez]
PHST- 2018/06/16 06:00 [pubmed]
PHST- 2018/06/16 06:01 [medline]
PHST- 2018/06/15 00:00 [pmc-release]
AID - 10.1186/s40634-018-0132-x [pii]
AID - 132 [pii]
AID - 10.1186/s40634-018-0132-x [doi]
PST - epublish
SO  - J Exp Orthop. 2018 Jun 15;5(1):21. doi: 10.1186/s40634-018-0132-x.