PMID- 29905040
OWN - NLM
STAT- MEDLINE
DCOM- 20190211
LR  - 20210915
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 32
IP  - 6
DP  - 2018 Jun 15
TI  - [Promotion of transplanted collagen scaffolds combined with brain-derived 
      neurotrophic factor for axonal regeneration and motor function recovery in rats 
      after transected spinal cord injury].
PG  - 650-659
LID - 10.7507/1002-1892.201803094 [doi]
AB  - OBJECTIVE: To evaluate the effect of the combination of collagen scaffold and 
      brain-derived neurotrophic factor (BDNF) on the repair of transected spinal cord 
      injury in rats. METHODS: Thirty-two Sprague-Dawley rats were randomly divided 
      into 4 groups: group A (sham operation group), T (9), T (10) segments of the 
      spinal cord was only exposed; group B, 4-mm T (9), T (10) segments of the spinal 
      cord were resected; group C, 4-mm T (9), T (10) segments of the spinal cord were 
      resected and linear ordered collagen scaffolds (LOCS) with corresponding length 
      was transplanted into lesion site; group D, 4-mm T (9), T (10) segments of the 
      spinal cord were resected and LOCS with collagen binding domain (CBD)-BDNF was 
      transplanted into lesion site. During 3 months after operation, 
      Basso-Beattie-Bresnahan (BBB) locomotor score assessment was performed for each 
      rat once a week. At 3 months after operation, electrophysiological test of motor 
      evoked potential (MEP) was performed for rats in each group. Subsequently, 
      retrograde tracing was performed for each rat by injection of fluorogold (FG) at 
      the L (2) spinal cord below the injury level. One week later, brains and spinal 
      cord tissues of rats were collected. Morphological observation was performed to 
      spinal cord tissues after dehydration. The thoracic spinal cords including lesion 
      area were collected and sliced horizontally. Thoracic spinal cords 1 cm above 
      lesion area and lumbar spinal cords 1 cm below lesion area were collected and 
      sliced coronally. Coronal spinal cord tissue sections were observed by the laser 
      confocal scanning microscope and calculated the integral absorbance ( IA) value 
      of FG-positive cells. Horizontal tissue sections of thoracic spinal cord 
      underwent immunofluorescence staining to observe the building of transected 
      spinal cord injury model, axonal regeneration in damaged area, and synapse 
      formation of regenerated axons. RESULTS: During 3 months after operation, the BBB 
      scores of groups B, C, and D were significantly lower than those of group A ( 
      P<0.05). The BBB scores of group D at 2-12 weeks after operation were 
      significantly higher than those of groups B and C ( P<0.05). Electrophysiological 
      tests revealed that there was no MEP in group B; the latencies of MEP in groups C 
      and D were significantly longer than that in group A ( P<0.05), and in group C 
      than in group D ( P<0.05). Morphological observation of spinal cord tissues 
      showed that the injured area of the spinal cord in group B extended to both two 
      ends, and the lesion site was severely damaged. The morphologies of spinal cord 
      tissues in groups C and D recovered well, and the morphology in group D was 
      closer to normal tissue. Results of retrograde tracing showed that the gray 
      matters of lumbar spinal cords below the lesion area in each group were filled 
      with FG-positive cells; in thoracic spinal cords above lesion sites, the IA value 
      of FG-positive cells in coronal section of spinal cord in group A was 
      significantly larger than those in groups B, C, and D ( P<0.05), and in groups C 
      and D than in group B ( P<0.05), but no significant difference was found between 
      groups C and D ( P>0.05). Immunofluorescence staining results of spinal cord 
      tissue sections selected from dorsal to ventral spinal cord showed transected 
      injured areas of spinal cords which were significantly different from normal 
      tissues. The numbers of NF-positive axons in lesion center of group A were 
      significantly larger than those of groups B, C, and D ( P<0.05), and in groups C 
      and D than in group B ( P<0.05), and in group D than in group C ( P<0.05). 
      CONCLUSION: The combined therapeutic approach containing LOCS and CBD-BDNF can 
      promote axonal regeneration and recovery of hind limb motor function after 
      transected spinal cord injury in rats.
FAU - Chen, Xi
AU  - Chen X
AD  - Institute of Combined Injury, College of Military Preventive Medicine, Army 
      Medical University (Third Military Medical University), Chongqing, 400038, 
      P.R.China.
FAU - Fan, Yongheng
AU  - Fan Y
AD  - National State Key Laboratory of Molecular Developmental Biology, Institute of 
      Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100190, 
      P.R.China.
FAU - Xiao, Zhifeng
AU  - Xiao Z
AD  - National State Key Laboratory of Molecular Developmental Biology, Institute of 
      Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100190, 
      P.R.China.
FAU - Li, Xing
AU  - Li X
AD  - National State Key Laboratory of Molecular Developmental Biology, Institute of 
      Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100190, 
      P.R.China.
FAU - Yang, Bin
AU  - Yang B
AD  - National State Key Laboratory of Molecular Developmental Biology, Institute of 
      Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100190, 
      P.R.China.
FAU - Zhao, Yannan
AU  - Zhao Y
AD  - National State Key Laboratory of Molecular Developmental Biology, Institute of 
      Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100190, 
      P.R.China.
FAU - Hou, Xianglin
AU  - Hou X
AD  - National State Key Laboratory of Molecular Developmental Biology, Institute of 
      Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100190, 
      P.R.China.
FAU - Han, Sufang
AU  - Han S
AD  - National State Key Laboratory of Molecular Developmental Biology, Institute of 
      Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100190, 
      P.R.China.
FAU - Dai, Jianwu
AU  - Dai J
AD  - Institute of Combined Injury, College of Military Preventive Medicine, Army 
      Medical University (Third Military Medical University), Chongqing, 400038, 
      P.R.China;National State Key Laboratory of Molecular Developmental Biology, 
      Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 
      Beijing, 100190, P.R.China.jwdai@genetics.ac.cn.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - *Brain-Derived Neurotrophic Factor
MH  - *Collagen
MH  - *Nerve Regeneration
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function
MH  - Regeneration
MH  - Spinal Cord
MH  - *Spinal Cord Injuries/therapy
MH  - *Tissue Scaffolds
PMC - PMC8413998
OTO - NOTNLM
OT  - Collagen scaffold
OT  - brain-derived neurotrophic factor
OT  - collagen binding domain
OT  - motor function
OT  - rat
OT  - spinal cord injury
EDAT- 2018/06/16 06:00
MHDA- 2019/02/12 06:00
PMCR- 2018/06/01
CRDT- 2018/06/16 06:00
PHST- 2018/06/16 06:00 [entrez]
PHST- 2018/06/16 06:00 [pubmed]
PHST- 2019/02/12 06:00 [medline]
PHST- 2018/06/01 00:00 [pmc-release]
AID - zgxfcjwkzz-32-6-650 [pii]
AID - 10.7507/1002-1892.201803094 [doi]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2018 Jun 15;32(6):650-659. doi: 
      10.7507/1002-1892.201803094.