PMID- 29905117
OWN - NLM
STAT- MEDLINE
DCOM- 20180629
LR  - 20240402
IS  - 1469-8714 (Electronic)
IS  - 0952-5238 (Print)
IS  - 0952-5238 (Linking)
VI  - 35
DP  - 2018 Jan
TI  - Melanopsin expression in the cornea.
PG  - E004
LID - 10.1017/S0952523817000359 [doi]
AB  - A unique class of intrinsically photosensitive retinal ganglion cells in 
      mammalian retinae has been recently discovered and characterized. These neurons 
      can generate visual signals in the absence of inputs from rods and cones, the 
      conventional photoreceptors in the visual system. These light sensitive ganglion 
      cells (mRGCs) express the non-rod, non-cone photopigment melanopsin and play well 
      documented roles in modulating pupil responses to light, photoentrainment of 
      circadian rhythms, mood, sleep and other adaptive light functions. While most 
      research efforts in mammals have focused on mRGCs in retina, recent studies 
      reveal that melanopsin is expressed in non-retinal tissues. For example, 
      light-evoked melanopsin activation in extra retinal tissue regulates pupil 
      constriction in the iris and vasodilation in the vasculature of the heart and 
      tail. As another example of nonretinal melanopsin expression we report here the 
      previously unrecognized localization of this photopigment in nerve fibers within 
      the cornea. Surprisingly, we were unable to detect light responses in the 
      melanopsin-expressing corneal fibers in spite of our histological evidence based 
      on genetically driven markers and antibody staining. We tested further for 
      melanopsin localization in cell bodies of the trigeminal ganglia (TG), the 
      principal nuclei of the peripheral nervous system that project sensory fibers to 
      the cornea, and found expression of melanopsin mRNA in a subset of TG neurons. 
      However, neither electrophysiological recordings nor calcium imaging revealed any 
      light responsiveness in the melanopsin positive TG neurons. Given that we found 
      no light-evoked activation of melanopsin-expressing fibers in cornea or in cell 
      bodies in the TG, we propose that melanopsin protein might serve other sensory 
      functions in the cornea. One justification for this idea is that melanopsin 
      expressed in Drosophila photoreceptors can serve as a temperature sensor.
FAU - Delwig, Anton
AU  - Delwig A
AD  - Department of Ophthalmology,School of Medicine,University of California San 
      Francisco,San Francisco,California.
FAU - Chaney, Shawnta Y
AU  - Chaney SY
AD  - Department of Ophthalmology,School of Medicine,University of California San 
      Francisco,San Francisco,California.
FAU - Bertke, Andrea S
AU  - Bertke AS
AD  - Proctor Foundation,School of Medicine,University of California San Francisco,San 
      Francisco,California.
FAU - Verweij, Jan
AU  - Verweij J
AD  - Department of Ophthalmology,School of Medicine,University of California San 
      Francisco,San Francisco,California.
FAU - Quirce, Susana
AU  - Quirce S
AD  - Instituto de Neurociencias de Alicante,Universidad Miguel Hernandez-CSIC,San Juan 
      de Alicante,Spain.
FAU - Larsen, Delaine D
AU  - Larsen DD
AD  - Department of Ophthalmology,School of Medicine,University of California San 
      Francisco,San Francisco,California.
FAU - Yang, Cindy
AU  - Yang C
AD  - Department of Anatomy,School of Medicine,University of California San 
      Francisco,San Francisco,California.
FAU - Buhr, Ethan
AU  - Buhr E
AD  - Department of Ophthalmology,School of Medicine,University of 
      Washington,Seattle,Washington.
FAU - VAN Gelder, Russell
AU  - VAN Gelder R
AD  - Department of Ophthalmology,School of Medicine,University of 
      Washington,Seattle,Washington.
FAU - Gallar, Juana
AU  - Gallar J
AD  - Instituto de Neurociencias de Alicante,Universidad Miguel Hernandez-CSIC,San Juan 
      de Alicante,Spain.
FAU - Margolis, Todd
AU  - Margolis T
AD  - Department of Ophthalmology,School of Medicine,University of California San 
      Francisco,San Francisco,California.
FAU - Copenhagen, David R
AU  - Copenhagen DR
AD  - Department of Ophthalmology,School of Medicine,University of California San 
      Francisco,San Francisco,California.
LA  - eng
GR  - R01 EY023179/EY/NEI NIH HHS/United States
GR  - T32 EY007120/EY/NEI NIH HHS/United States
GR  - R01 EY001869/EY/NEI NIH HHS/United States
GR  - R21 EY025435/EY/NEI NIH HHS/United States
GR  - R01 EY022697/EY/NEI NIH HHS/United States
GR  - P30 EY002162/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Vis Neurosci
JT  - Visual neuroscience
JID - 8809466
RN  - 0 (RNA, Messenger)
RN  - 0 (Rod Opsins)
RN  - 0 (melanopsin)
SB  - IM
MH  - Animals
MH  - Cell Body/metabolism
MH  - Cells, Cultured
MH  - Cornea/*metabolism
MH  - Dependovirus/genetics
MH  - Electrophysiology
MH  - Female
MH  - Gene Expression Regulation/*physiology
MH  - Guinea Pigs
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Nerve Fibers/metabolism
MH  - RNA, Messenger/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Rod Opsins/*genetics/metabolism
MH  - Transfection
MH  - Trigeminal Ganglion/*metabolism
PMC - PMC6203320
MID - NIHMS986607
OTO - NOTNLM
OT  - Cornea
OT  - Melanopsin
OT  - Photosensitivity
OT  - Trigeminal ganglion
EDAT- 2018/06/16 06:00
MHDA- 2018/06/30 06:00
PMCR- 2018/10/26
CRDT- 2018/06/16 06:00
PHST- 2018/06/16 06:00 [entrez]
PHST- 2018/06/16 06:00 [pubmed]
PHST- 2018/06/30 06:00 [medline]
PHST- 2018/10/26 00:00 [pmc-release]
AID - S0952523817000359 [pii]
AID - 10.1017/S0952523817000359 [doi]
PST - ppublish
SO  - Vis Neurosci. 2018 Jan;35:E004. doi: 10.1017/S0952523817000359.