PMID- 29908114
OWN - NLM
STAT- MEDLINE
DCOM- 20180913
LR  - 20181202
IS  - 0513-4870 (Print)
IS  - 0513-4870 (Linking)
VI  - 51
IP  - 11
DP  - 2016 Nov
TI  - [Inhibitory effect of oleanolic acid on inflammatory response in IL-1beta-stimulated 
      human synovial sarcoma SW982 cells].
PG  - 1711-6
AB  - To study the role of oleanolic acid on interleukin (IL)-1beta-stimulated expression 
      of inflammatory cytokines, and to explore its anti-inflammatory mechanism in 
      SW982 cells, the toxicity of oleanolic acid on SW982 cells was detected by MTT; 
      effects of different concentrations of oleanolic acid(5, 10, 20 mumol.L(-1)) on 
      the expression of inflammatory factors IL-6, IL-8 and matrix metalloproteinase-1 
      (MMP-1) was tested at protein and m RNA levels. The study was performed in 
      IL-1beta-stimulated SW982 cells together with enzyme-linked immunosorbent assay 
      (ELISA) and real-time fluorescence quantitative PCR (real-time PCR) methods; the 
      influence of oleanolic acid on the phosphorylation of mitogen-activated protein 
      kinase (MAPK), phosphatidyl inositol-3-kinase/Akt (PI3K/Akt) and nuclear 
      transcription factor-kappaB (NF-kappaB) signaling pathways related protein was analyzed 
      by Western blot. Results showed that different concentrations of oleanolic 
      acid(</=40 mumol.L(-1)) were almost non-toxicity to SW982 cells; oleanolic acid 
      significantly inhibited the expression of inflammatory factors in a 
      dose-dependent manner; oleanolic acid restrained extracellular signal-related 
      kinase (ERK), p38, c-jun N-terminal kinase (JNK) and Akt protein phosphorylation 
      and IkappaB-alpha protein degradation obviously. The inhibition effect of oleanolic acid 
      on inflammatory factors stimulated by IL-1beta may be worked through MAPK, PI3K/Akt 
      and NF-kappaB signaling pathways.
FAU - Lian, Jun-jiang
AU  - Lian JJ
FAU - Cheng, Bin-feng
AU  - Cheng BF
FAU - Gao, Yao-xin
AU  - Gao YX
FAU - Zhang, Ya-ping
AU  - Zhang YP
FAU - Ma, Shuang-ping
AU  - Ma SP
FAU - Zhang, Bin-bin
AU  - Zhang BB
FAU - Guo, Dan-dan
AU  - Guo DD
FAU - Xue, Han
AU  - Xue H
FAU - Feng, Zhi-wei
AU  - Feng ZW
LA  - chi
PT  - Journal Article
PL  - China
TA  - Yao Xue Xue Bao
JT  - Yao xue xue bao = Acta pharmaceutica Sinica
JID - 21710340R
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (Interleukin-1beta)
RN  - 0 (NF-kappa B)
RN  - 0 (NFKBIA protein, human)
RN  - 0 (RELA protein, human)
RN  - 0 (Transcription Factor RelA)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - 6SMK8R7TGJ (Oleanolic Acid)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 3.4.24.7 (MMP1 protein, human)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Cell Line
MH  - Cytokines/metabolism
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Humans
MH  - I-kappa B Proteins/metabolism
MH  - Inflammation/*metabolism
MH  - Interleukin-1beta/pharmacology
MH  - Matrix Metalloproteinase 1/metabolism
MH  - NF-KappaB Inhibitor alpha/metabolism
MH  - NF-kappa B/metabolism
MH  - Oleanolic Acid/*pharmacology
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Sarcoma, Synovial/drug therapy/*metabolism
MH  - Signal Transduction
MH  - Transcription Factor RelA/metabolism
EDAT- 2016/11/01 00:00
MHDA- 2018/09/14 06:00
CRDT- 2018/06/17 06:00
PHST- 2018/06/17 06:00 [entrez]
PHST- 2016/11/01 00:00 [pubmed]
PHST- 2018/09/14 06:00 [medline]
PST - ppublish
SO  - Yao Xue Xue Bao. 2016 Nov;51(11):1711-6.