PMID- 29909072
OWN - NLM
STAT- MEDLINE
DCOM- 20190701
LR  - 20190701
IS  - 1090-2163 (Electronic)
IS  - 0008-8749 (Linking)
VI  - 331
DP  - 2018 Sep
TI  - Bone marrow-derived inflammatory and steady state DCs are different in both 
      functions and survival.
PG  - 100-109
LID - S0008-8749(18)30204-1 [pii]
LID - 10.1016/j.cellimm.2018.06.001 [doi]
AB  - Dendritic cells (DCs) contain heterogeneous populations, with classical DCs 
      developed at steady state and monocyte-derived DCs mobilized under inflammatory 
      conditions, although their total numbers in vivo are scares. To obtain enough 
      quantity for immunological study or clinical application, we have previously 
      established that bone marrow-derived DCs in the presence of Flt-3L (FL-DCs) or 
      GM-CSF (GM-DCs) in vitro are equivalent to the steady state DCs and inflammatory 
      DCs in vivo respectively. What difference, however, exists between these two most 
      commonly used culture systems in DC functions and survival, and how does it 
      correlate to the division of works by their corresponding counterparts in vivo 
      remain ill-defined. In this study, we found that GM-DCs of inflammatory nature 
      were more phagocytic, potent at inducing Th2 and Th17 differentiation, and had 
      longer survival rate, whereas FL-DCs of steady state characters were stronger T 
      cell activator and better at directing Th1 differentiation. Mechanistically, NO 
      production induced by the LPS-activated GM-DCs could partly explain for their 
      failure to improve T cell proliferation, and the distinct T cell differentiation 
      profiles and viability demonstrated by the two types of DCs were underpinned by 
      their preferential secretion of T cell polarizing cytokines and expression of 
      anti-apoptotic genes. Such disparate functionalities and survival potentials of 
      steady state and inflammatory DCs in vitro fit in well with their respective 
      roles in vivo in particular immunological settings and have serious implications 
      in translational applications.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Zhang, Wenjie
AU  - Zhang W
AD  - Anhui Provincial Key Laboratory for Conservation and Exploitation of Biological 
      Resources, School of Life Science, Anhui Normal University, Wuhu 241000, China.
FAU - Ding, Ying
AU  - Ding Y
AD  - Anhui Provincial Key Laboratory for Conservation and Exploitation of Biological 
      Resources, School of Life Science, Anhui Normal University, Wuhu 241000, China.
FAU - Sun, Li
AU  - Sun L
AD  - Anhui Provincial Key Laboratory for Conservation and Exploitation of Biological 
      Resources, School of Life Science, Anhui Normal University, Wuhu 241000, China.
FAU - Hong, Qing
AU  - Hong Q
AD  - Anhui Provincial Key Laboratory for Conservation and Exploitation of Biological 
      Resources, School of Life Science, Anhui Normal University, Wuhu 241000, China.
FAU - Sun, Yumei
AU  - Sun Y
AD  - Anhui Provincial Key Laboratory for Conservation and Exploitation of Biological 
      Resources, School of Life Science, Anhui Normal University, Wuhu 241000, China.
FAU - Han, Liangliang
AU  - Han L
AD  - Anhui Provincial Key Laboratory for Conservation and Exploitation of Biological 
      Resources, School of Life Science, Anhui Normal University, Wuhu 241000, China.
FAU - Zi, Mengting
AU  - Zi M
AD  - Anhui Provincial Key Laboratory for Conservation and Exploitation of Biological 
      Resources, School of Life Science, Anhui Normal University, Wuhu 241000, China.
FAU - Xu, Yuekang
AU  - Xu Y
AD  - Anhui Provincial Key Laboratory for Conservation and Exploitation of Biological 
      Resources, School of Life Science, Anhui Normal University, Wuhu 241000, China. 
      Electronic address: yuekang.xu@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180607
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Cytokines)
RN  - 0 (Membrane Proteins)
RN  - 0 (flt3 ligand protein)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/*immunology/metabolism
MH  - Cell Differentiation/drug effects/*immunology
MH  - Cell Survival/immunology
MH  - Cells, Cultured
MH  - Cytokines/*immunology/metabolism
MH  - Dendritic Cells/cytology/*immunology/metabolism
MH  - Female
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Lymphocyte Activation/immunology
MH  - Membrane Proteins/pharmacology
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - T-Lymphocytes/cytology/immunology/metabolism
OTO - NOTNLM
OT  - Dendritic cells
OT  - GM-CSF
OT  - Inflammation
OT  - T cell differentiation
OT  - T cell proliferation
EDAT- 2018/06/18 06:00
MHDA- 2019/07/02 06:00
CRDT- 2018/06/18 06:00
PHST- 2017/10/15 00:00 [received]
PHST- 2018/05/06 00:00 [revised]
PHST- 2018/06/06 00:00 [accepted]
PHST- 2018/06/18 06:00 [pubmed]
PHST- 2019/07/02 06:00 [medline]
PHST- 2018/06/18 06:00 [entrez]
AID - S0008-8749(18)30204-1 [pii]
AID - 10.1016/j.cellimm.2018.06.001 [doi]
PST - ppublish
SO  - Cell Immunol. 2018 Sep;331:100-109. doi: 10.1016/j.cellimm.2018.06.001. Epub 2018 
      Jun 7.