PMID- 29909133
OWN - NLM
STAT- MEDLINE
DCOM- 20181016
LR  - 20181016
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 36
IP  - 30
DP  - 2018 Jul 16
TI  - Tuberculin skin test reaction is related to memory, but not naive CD4(+) T cell 
      responses to mycobacterial stimuli in BCG-vaccinated young adults.
PG  - 4566-4577
LID - S0264-410X(18)30708-4 [pii]
LID - 10.1016/j.vaccine.2018.05.068 [doi]
AB  - Bacillus Calmette-Guerin (BCG) is the only vaccine available against tuberculosis 
      and the tuberculin skin test (TST) is the most widely used method to detect BCG 
      take. However, subjects may remain TST-negative, even after several BCG 
      administrations. To investigate some of the potential reasons underlying this 
      inability of developing tuberculin sensitivity in response to BCG we compared the 
      effect of different mycobacterial stimuli in the groups differently responding to 
      tuberculin. TST was performed on 71 healthy adults aged 25-30 years, who had 
      received BCG in their childhood, and considered TST-positive at >/=10 mm. Dendritic 
      cells (DCs) were incubated with PPD, live BCG or rBCGhIL-18, producing human 
      IL-18. The latter strain was used to investigate whether the production of IL-18 
      could overcome some of the immune read-out limitations in the TST-negative 
      subjects. CD86, CD80, CD40, and DC-specific intracellular adhesion molecule-3 
      grabbing nonintegrin (DC-SIGN) expression was analysed by flow cytometry and 
      IL-10, IL-23 and IP-10 secretion in culture supernatants by ELISA. In DCs-T cell 
      co-cultures with naive and memory CD4(+) T cells, the IFN-gamma and IL-10 levels were 
      determined by ELISA. We found no difference in IL-10 and IFN-gamma production by 
      naive T cells between the TST-negative and TST-positive subjects. However, IFN-gamma 
      was produced in significantly higher amounts by memory T cells incubated with 
      PPD, BCG or rBCGhIL-18-pulsed DCs in TST-positive than in TST-negative subjects, 
      whereas the numbers of the IFN-gamma-producing T cells were similar in both groups. 
      This difference may be partially due to a decreased CD40 and enhanced reduction 
      in DC-SIGN expression by DCs of TST-negative versus TST-positive subjects. A 
      strong effect of IL-18 expression by rBCGhIL-18 on IL-23 production by the DC was 
      seen in both groups, which likely was the reason for the increased IFN-gamma 
      production by naive T cells upon incubation with mycobacteria-pulsed DC, 
      regardless of the TST status.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Kowalewicz-Kulbat, Magdalena
AU  - Kowalewicz-Kulbat M
AD  - Department of Immunology and Infectious Biology, Institute of Microbiology, 
      Biotechnology and Immunology, Faculty of Biology and Environmental Protection, 
      University of Lodz, Lodz, Poland. Electronic address: 
      magdalena.kowalewicz@biol.uni.lodz.pl.
FAU - Szpakowski, Piotr
AU  - Szpakowski P
AD  - Department of Immunology and Infectious Biology, Institute of Microbiology, 
      Biotechnology and Immunology, Faculty of Biology and Environmental Protection, 
      University of Lodz, Lodz, Poland; Department of Neurology and Stroke, Medical 
      University of Lodz, Lodz, Poland.
FAU - Locht, Camille
AU  - Locht C
AD  - Department of Immunology and Infectious Biology, Institute of Microbiology, 
      Biotechnology and Immunology, Faculty of Biology and Environmental Protection, 
      University of Lodz, Lodz, Poland; Center for Infection and Immunity of Lille, 
      Institut Pasteur de Lille, Lille, France; Inserm U1019, Lille, France; CNRS UMR 
      8204, Lille, France; Universite Lille Nord de France, Lille, France.
FAU - Biet, Franck
AU  - Biet F
AD  - UMR1282 Infectiologie et Sante Publique (ISP-311), INRA Centre Val de Loire, 
      Nouzilly, France.
FAU - Kaplonek, Paulina
AU  - Kaplonek P
AD  - Department of Immunology and Infectious Biology, Institute of Microbiology, 
      Biotechnology and Immunology, Faculty of Biology and Environmental Protection, 
      University of Lodz, Lodz, Poland.
FAU - Krawczyk, Krzysztof T
AU  - Krawczyk KT
AD  - Department of Immunology and Infectious Biology, Institute of Microbiology, 
      Biotechnology and Immunology, Faculty of Biology and Environmental Protection, 
      University of Lodz, Lodz, Poland.
FAU - Pestel, Joel
AU  - Pestel J
AD  - Universite Lille Nord de France, Lille, France; CNRS-UMR 8576, Unite de 
      Glycobiologie Structurale et Fonctionnelle, IFR 147, Universite Lille Nord de 
      France, Universite de Lille 1, Villeneuve d'Ascq, France.
FAU - Rudnicka, Wieslawa
AU  - Rudnicka W
AD  - Department of Immunology and Infectious Biology, Institute of Microbiology, 
      Biotechnology and Immunology, Faculty of Biology and Environmental Protection, 
      University of Lodz, Lodz, Poland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180619
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (BCG Vaccine)
SB  - IM
MH  - Adult
MH  - BCG Vaccine/immunology
MH  - CD4-Positive T-Lymphocytes
MH  - Dendritic Cells/immunology
MH  - Female
MH  - Humans
MH  - Male
MH  - Monocytes/immunology
MH  - T-Lymphocyte Subsets/immunology
MH  - Tuberculin Test/*methods
MH  - Young Adult
OTO - NOTNLM
OT  - BCG
OT  - Cytokines
OT  - Dendritic cells (DCs)
OT  - T cells
OT  - Tuberculin skin test (TST)
OT  - rBCGhIL-18
EDAT- 2018/06/18 06:00
MHDA- 2018/10/17 06:00
CRDT- 2018/06/18 06:00
PHST- 2018/01/23 00:00 [received]
PHST- 2018/05/11 00:00 [revised]
PHST- 2018/05/13 00:00 [accepted]
PHST- 2018/06/18 06:00 [pubmed]
PHST- 2018/10/17 06:00 [medline]
PHST- 2018/06/18 06:00 [entrez]
AID - S0264-410X(18)30708-4 [pii]
AID - 10.1016/j.vaccine.2018.05.068 [doi]
PST - ppublish
SO  - Vaccine. 2018 Jul 16;36(30):4566-4577. doi: 10.1016/j.vaccine.2018.05.068. Epub 
      2018 Jun 19.