PMID- 29909434
OWN - NLM
STAT- MEDLINE
DCOM- 20190322
LR  - 20190322
IS  - 1432-1017 (Electronic)
IS  - 0175-7571 (Linking)
VI  - 47
IP  - 7
DP  - 2018 Oct
TI  - Ionomer and protein size analysis by analytical ultracentrifugation and 
      electrospray scanning mobility particle sizer.
PG  - 777-787
LID - 10.1007/s00249-018-1314-2 [doi]
AB  - By combining analytical ultracentrifugation (AUC) in liquid phase and scanning 
      mobility particle sizer (SMPS) in the gas phase, additional information on the 
      particle size and morphology has been obtained for rigid particles. In this 
      paper, we transfer this concept to soft particles, allowing us to analyze the 
      size and molar mass of the short side chain perfluorosulfonic acid ionomer 
      Aquivion((R)) in a dilute aqueous suspension. The determination of the primary size 
      and exact molar mass of this class of polymers is challenging since they are 
      optically transparent and due to the formation of different aggregate structures 
      depending on the concentration and solvent properties. First, validation of AUC 
      and SMPS measurements was carried out using the well-defined biopolymers bovine 
      serum albumin (BSA) and lysozyme (LYZ) to confirm the reliability of the results 
      of the two unique and independent classifying methods. Then, the ionomer 
      Aquivion((R)) was studied using both techniques. From the mean molar mass of 
      185 +/- 14 kDa obtained by AUC, a mean hydrodynamic diameter of 7.6 +/- 0.5 nm was 
      calculated. The particle size obtained from SMPS (7.1 nm) agrees very well with 
      the results from AUC showing that the molecule was transferred into the gas phase 
      without significantly changing its structure. In conclusion, the Aquivion((R)) is 
      molecularly dispersed in the used aqueous buffer solution without any aggregate 
      formation in the investigated concentration range (< 2 g l(-1)).
FAU - Wawra, Simon E
AU  - Wawra SE
AD  - Institute of Particle Technology (LFG), Friedrich-Alexander-Universitat 
      Erlangen-Nurnberg (FAU), Cauerstrasse 4, 91058, Erlangen, Germany.
FAU - Thoma, Martin
AU  - Thoma M
AD  - Institute of Particle Technology (LFG), Friedrich-Alexander-Universitat 
      Erlangen-Nurnberg (FAU), Cauerstrasse 4, 91058, Erlangen, Germany.
FAU - Walter, Johannes
AU  - Walter J
AD  - Institute of Particle Technology (LFG), Friedrich-Alexander-Universitat 
      Erlangen-Nurnberg (FAU), Cauerstrasse 4, 91058, Erlangen, Germany.
AD  - Interdisciplinary Center for Functional Particle Systems (FPS), 
      Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Haberstrasse 9a, 91058, 
      Erlangen, Germany.
FAU - Lubbert, Christian
AU  - Lubbert C
AD  - Institute of Particle Technology (LFG), Friedrich-Alexander-Universitat 
      Erlangen-Nurnberg (FAU), Cauerstrasse 4, 91058, Erlangen, Germany.
AD  - Interdisciplinary Center for Functional Particle Systems (FPS), 
      Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Haberstrasse 9a, 91058, 
      Erlangen, Germany.
FAU - Thajudeen, Thaseem
AU  - Thajudeen T
AD  - Institute of Particle Technology (LFG), Friedrich-Alexander-Universitat 
      Erlangen-Nurnberg (FAU), Cauerstrasse 4, 91058, Erlangen, Germany.
AD  - Interdisciplinary Center for Functional Particle Systems (FPS), 
      Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Haberstrasse 9a, 91058, 
      Erlangen, Germany.
AD  - School of Mechanical Sciences, Indian Institute of Technology Goa, Ponda, 403401, 
      India.
FAU - Damm, Cornelia
AU  - Damm C
AD  - Institute of Particle Technology (LFG), Friedrich-Alexander-Universitat 
      Erlangen-Nurnberg (FAU), Cauerstrasse 4, 91058, Erlangen, Germany.
FAU - Peukert, Wolfgang
AU  - Peukert W
AUID- ORCID: 0000-0002-2847-107X
AD  - Institute of Particle Technology (LFG), Friedrich-Alexander-Universitat 
      Erlangen-Nurnberg (FAU), Cauerstrasse 4, 91058, Erlangen, Germany. 
      wolfgang.peukert@fau.de.
AD  - Interdisciplinary Center for Functional Particle Systems (FPS), 
      Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Haberstrasse 9a, 91058, 
      Erlangen, Germany. wolfgang.peukert@fau.de.
LA  - eng
GR  - DBF01253/Helmholtz-Gemeinschaft/
GR  - Cluster of Excellence "Engineering of Advanced Materials"/Deutsche 
      Forschungsgemeinschaft/
GR  - PE 427/28-2/Deutsche Forschungsgemeinschaft/
GR  - Fellowship/Alexander von Humboldt-Stiftung/
GR  - CA15126/Arbre Mobieu, Cost/
PT  - Journal Article
DEP - 20180616
PL  - Germany
TA  - Eur Biophys J
JT  - European biophysics journal : EBJ
JID - 8409413
RN  - 0 (Polymers)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Molecular Weight
MH  - Muramidase/chemistry
MH  - *Particle Size
MH  - Polymers/*chemistry
MH  - Serum Albumin, Bovine/*chemistry
MH  - Ultracentrifugation/instrumentation/*methods
OTO - NOTNLM
OT  - Analytical ultracentrifugation
OT  - Combined analysis
OT  - Multiwavelength detector
OT  - Scanning mobility particle sizer
EDAT- 2018/06/18 06:00
MHDA- 2019/03/23 06:00
CRDT- 2018/06/18 06:00
PHST- 2017/12/31 00:00 [received]
PHST- 2018/06/07 00:00 [accepted]
PHST- 2018/04/17 00:00 [revised]
PHST- 2018/06/18 06:00 [pubmed]
PHST- 2019/03/23 06:00 [medline]
PHST- 2018/06/18 06:00 [entrez]
AID - 10.1007/s00249-018-1314-2 [pii]
AID - 10.1007/s00249-018-1314-2 [doi]
PST - ppublish
SO  - Eur Biophys J. 2018 Oct;47(7):777-787. doi: 10.1007/s00249-018-1314-2. Epub 2018 
      Jun 16.