PMID- 29911266
OWN - NLM
STAT- MEDLINE
DCOM- 20190212
LR  - 20220408
IS  - 1559-0291 (Electronic)
IS  - 0273-2289 (Linking)
VI  - 187
IP  - 1
DP  - 2019 Jan
TI  - One-Step Bioprocess of Inulin to Product Inulo-Oligosaccharides Using Bacillus 
      subtilis Secreting an Extracellular Endo-Inulinase.
PG  - 116-128
LID - 10.1007/s12010-018-2806-3 [doi]
AB  - Inulo-oligosaccharides (IOSs), a novel food additive and health product, 
      represent a promising alternative to antibiotics. As prebiotics, IOSs can be 
      obtained from inulin by endo-inulinase-mediated hydrolysis. Nonetheless, 
      enzymatic catalysis is not feasible industrially because of the required 
      catalytic conditions and cost. In this study, a 2331-bp optimized gene inuQ (from 
      Pseudomonas mucidolens) encoding endo-inulinase was cloned into shuttle vector 
      PHY300PLK and transfected into Bacillus subtilis WB800-R, with the simultaneous 
      deletion of gene sacC encoding levanase. The maximal IOS yield after hydrolysis 
      of the crude extract of inulin was 67.84 +/- 0.72 g/L for a recombinant strain with 
      the signal peptide nprB from alkaline protease and promoter P(43). The conversion 
      rate reached 75.38%. For the major IOSs, the degree of polymerization was between 
      3 and 5. This study offers a simple and efficient one-step bioprocess for IOS 
      production from inulin through secretion of an extracellular heterologous 
      endo-inulinase by B. subtilis.
FAU - Jiang, Ruifan
AU  - Jiang R
AD  - State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food 
      Science and Light Industry, Nanjing Tech University, Nanjing, 211816, People's 
      Republic of China.
AD  - School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng, 
      224051, People's Republic of China.
FAU - Qiu, Yibin
AU  - Qiu Y
AD  - State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food 
      Science and Light Industry, Nanjing Tech University, Nanjing, 211816, People's 
      Republic of China.
FAU - Huang, Weiwei
AU  - Huang W
AD  - State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food 
      Science and Light Industry, Nanjing Tech University, Nanjing, 211816, People's 
      Republic of China.
FAU - Zhang, Li
AU  - Zhang L
AD  - School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng, 
      224051, People's Republic of China.
FAU - Xue, Feng
AU  - Xue F
AD  - School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng, 
      224051, People's Republic of China.
FAU - Ni, Hao
AU  - Ni H
AD  - School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng, 
      224051, People's Republic of China.
FAU - Mei, Difen
AU  - Mei D
AD  - School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng, 
      224051, People's Republic of China.
FAU - Gao, Jian
AU  - Gao J
AD  - School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng, 
      224051, People's Republic of China. gaojian@ycit.edu.cn.
FAU - Xu, Hong
AU  - Xu H
AD  - State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food 
      Science and Light Industry, Nanjing Tech University, Nanjing, 211816, People's 
      Republic of China. xuh@njtech.edu.cn.
LA  - eng
GR  - Grant 21376203/National Natural Science Foundation of China/
GR  - Grant JHB2011-54/the Promotion Program of Achievements in Scientific Research for 
      Industrial Production of Higher Education of Jiangsu Province/
GR  - Grant BE2012394/Qinglan Project of Higher Education of Jiangsu Province, Overseas 
      Research & Training Program for University Prominent Young & Middle-aged Teachers 
      and Presidents of Jiangsu Province, Jiangsu Agricultural Key Technology Research 
      and Development Program/
PT  - Journal Article
DEP - 20180618
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (Bacterial Proteins)
RN  - 0 (Oligosaccharides)
RN  - 0 (Recombinant Proteins)
RN  - 9005-80-5 (Inulin)
RN  - EC 3.2.1.- (Glycoside Hydrolases)
RN  - EC 3.2.1.7 (inulinase)
SB  - IM
MH  - Bacillus subtilis/*enzymology/genetics
MH  - Bacterial Proteins/biosynthesis/*chemistry/genetics
MH  - Glycoside Hydrolases/biosynthesis/*chemistry/genetics
MH  - Inulin/*chemistry
MH  - Oligosaccharides/*chemical synthesis/chemistry
MH  - Pseudomonas/enzymology/*genetics
MH  - Recombinant Proteins/biosynthesis/chemistry/genetics
OTO - NOTNLM
OT  - Bacillus subtilis
OT  - Endo-inulinase
OT  - Inulin
OT  - Inulo-oligosaccharides
EDAT- 2018/06/19 06:00
MHDA- 2019/02/13 06:00
CRDT- 2018/06/19 06:00
PHST- 2018/03/31 00:00 [received]
PHST- 2018/06/03 00:00 [accepted]
PHST- 2018/06/19 06:00 [pubmed]
PHST- 2019/02/13 06:00 [medline]
PHST- 2018/06/19 06:00 [entrez]
AID - 10.1007/s12010-018-2806-3 [pii]
AID - 10.1007/s12010-018-2806-3 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2019 Jan;187(1):116-128. doi: 10.1007/s12010-018-2806-3. 
      Epub 2018 Jun 18.