PMID- 29912041
OWN - NLM
STAT- MEDLINE
DCOM- 20180831
LR  - 20180831
IS  - 1536-4798 (Electronic)
IS  - 0277-3740 (Linking)
VI  - 37
IP  - 9
DP  - 2018 Sep
TI  - Cincinnati Protocol for Preoperative Screening and Donor Selection for Ocular 
      Surface Stem Cell Transplantation.
PG  - 1192-1197
LID - 10.1097/ICO.0000000000001662 [doi]
AB  - PURPOSE: To describe our process for preoperative screening and donor selection 
      for ocular surface stem cell transplantation (OSST). METHODS: A 7-year 
      retrospective chart review was performed on limbal stem cell deficiency patients. 
      The inclusion criterion was all patients who underwent an OSST procedure. The 
      exclusion criterion was eyes with unilateral disease in which an autograft was 
      performed. Data for human leukocyte antigen (HLA) typing, virtual crossmatching, 
      donor-specific antibody, and panel reactive antibody level were obtained. 
      RESULTS: Of the included 142 eyes (104 patients), 19 patients had no recorded 
      living donor availability data, and HLA typing was not performed on 16 patients. 
      A total of 94 donors (mean 1.4 donors/patient, range 1-6) were tested for 67 
      recipients. For 2 patients with graft-versus-host disease, no further HLA typing 
      was needed, as the donors were known HLA-identical donors. For 47 patients, only 
      1 donor was tested, whereas multiple donors underwent HLA typing for 20 patients. 
      There were 73 ABO (blood group)-compatible matches for the 61 tested recipients, 
      and only 1 recipient did not have any ABO-compatible donor. For the virtual 
      crossmatch, there were 5 patients who did not have a compatible donor (positive 
      virtual crossmatch). The best available donor match was a sibling for 41 
      recipients (65%), a parent for 19 recipients (30%), and an offspring for 3 
      recipients (5%). CONCLUSIONS: Our protocol for OSST preoperative screening and 
      donor selection minimizes the antigenic burden for transplanted tissue by 
      selecting the best available donor match.
FAU - Cheung, Albert Y
AU  - Cheung AY
AD  - Cincinnati Eye Institute, University of Cincinnati, Department of Ophthalmology, 
      Cincinnati, OH.
AD  - Virginia Eye Consultants, Norfolk, VA.
FAU - Sarnicola, Enrica
AU  - Sarnicola E
AD  - Cincinnati Eye Institute, University of Cincinnati, Department of Ophthalmology, 
      Cincinnati, OH.
AD  - Clinica Degli Occhi Sarnicola, Grosseto, Italy.
FAU - Kurji, Khaliq H
AU  - Kurji KH
AD  - Cincinnati Eye Institute, University of Cincinnati, Department of Ophthalmology, 
      Cincinnati, OH.
FAU - Govil, Amit
AU  - Govil A
AD  - Division of Nephrology and Hypertension, University of Cincinnati, Cincinnati, 
      OH.
FAU - Mogilishetty, Gautham
AU  - Mogilishetty G
AD  - Lee Physician Group, Fort Myers, FL.
FAU - Eslani, Medi
AU  - Eslani M
AD  - Cincinnati Eye Institute, University of Cincinnati, Department of Ophthalmology, 
      Cincinnati, OH.
FAU - Wright, Elizabeth
AU  - Wright E
AD  - Cincinnati Eye Institute, University of Cincinnati, Department of Ophthalmology, 
      Cincinnati, OH.
FAU - Brailey, Paul
AU  - Brailey P
AD  - Hoxworth Blood Center, Cincinnati, OH.
FAU - Holland, Edward J
AU  - Holland EJ
AD  - Cincinnati Eye Institute, University of Cincinnati, Department of Ophthalmology, 
      Cincinnati, OH.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cornea
JT  - Cornea
JID - 8216186
RN  - 0 (ABO Blood-Group System)
SB  - IM
MH  - ABO Blood-Group System
MH  - Adult
MH  - Blood Grouping and Crossmatching
MH  - Clinical Protocols
MH  - Corneal Diseases/surgery
MH  - Donor Selection/*methods
MH  - Female
MH  - Graft Survival
MH  - Histocompatibility Testing
MH  - Humans
MH  - Limbus Corneae/*cytology
MH  - *Living Donors
MH  - Male
MH  - Middle Aged
MH  - Retrospective Studies
MH  - *Stem Cell Transplantation
EDAT- 2018/06/19 06:00
MHDA- 2018/09/01 06:00
CRDT- 2018/06/19 06:00
PHST- 2018/06/19 06:00 [pubmed]
PHST- 2018/09/01 06:00 [medline]
PHST- 2018/06/19 06:00 [entrez]
AID - 10.1097/ICO.0000000000001662 [doi]
PST - ppublish
SO  - Cornea. 2018 Sep;37(9):1192-1197. doi: 10.1097/ICO.0000000000001662.