PMID- 29912589
OWN - NLM
STAT- Publisher
LR  - 20240227
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Print)
IS  - 0892-6638 (Linking)
VI  - 32
IP  - 11
DP  - 2018 Jun 18
TI  - Lactobacillus plantarum prevents and mitigates alcohol-induced disruption of 
      colonic epithelial tight junctions, endotoxemia, and liver damage by an EGF 
      receptor-dependent mechanism.
PG  - fj201800351R
LID - 10.1096/fj.201800351R [doi]
AB  - Pathogenesis of alcohol-related diseases such as alcoholic hepatitis involves gut 
      barrier dysfunction, endotoxemia, and toxin-mediated cellular injury. Here we 
      show that Lactobacillus plantarum not only blocks but also mitigates ethanol 
      (EtOH)-induced gut and liver damage in mice. L. plantarum blocks EtOH-induced 
      protein thiol oxidation, and down-regulation of antioxidant gene expression in 
      colon L. plantarum also blocks EtOH-induced expression of TNF-alpha, IL-1beta, IL-6, 
      monocyte chemotactic protein 1 ( MCP1), C-X-C motif chemokine ligand ( CXCL)1, 
      and CXCL2 genes in colon. Epidermal growth factor receptor (EGFR) signaling 
      mediates the L. plantarum-mediated protection of tight junctions (TJs) and 
      barrier function from acetaldehyde, the EtOH metabolite, in Caco-2 cell 
      monolayers. In mice, doxycycline-mediated expression of dominant negative EGFR 
      blocks L. plantarum-mediated prevention of EtOH-induced TJ disruption, mucosal 
      barrier dysfunction, oxidative stress, and inflammatory response in colon. L. 
      plantarum blocks EtOH-induced endotoxemia as well as EtOH-induced pathologic 
      lesions, triglyceride deposition, oxidative stress, and inflammatory responses in 
      the liver by an EGFR-dependent mechanism. L. plantarum treatment after injury 
      accelerated recovery from EtOH-induced TJ, barrier dysfunction, oxidative stress, 
      and inflammatory response in colon, endotoxemia, and liver damage. Results 
      demonstrate that L. plantarum has both preventive and therapeutic values in 
      treatment of alcohol-induced tissue injury, particularly in alcoholic 
      hepatitis.-Shukla, P. K., Meena, A. S., Manda, B., Gomes-Solecki, M., Dietrich, 
      P., Dragatsis, I., Rao, R. Lactobacillus plantarum prevents and mitigates 
      alcohol-induced disruption of colonic epithelial tight junctions, endotoxemia, 
      and liver damage by an EGF receptor-dependent mechanism.
FAU - Shukla, Pradeep K
AU  - Shukla PK
AD  - Department of Physiology, University of Tennessee Health Science Center, Memphis, 
      Tennessee, USA.
FAU - Meena, Avtar S
AU  - Meena AS
AD  - Department of Physiology, University of Tennessee Health Science Center, Memphis, 
      Tennessee, USA.
FAU - Manda, Bhargavi
AU  - Manda B
AD  - Department of Physiology, University of Tennessee Health Science Center, Memphis, 
      Tennessee, USA.
FAU - Gomes-Solecki, Maria
AU  - Gomes-Solecki M
AD  - Department of Microbiology, Immunology and Biochemistry, University of Tennessee 
      Health Science Center, Memphis, Tennessee, USA.
FAU - Dietrich, Paula
AU  - Dietrich P
AD  - Department of Physiology, University of Tennessee Health Science Center, Memphis, 
      Tennessee, USA.
FAU - Dragatsis, Ioannis
AU  - Dragatsis I
AD  - Department of Physiology, University of Tennessee Health Science Center, Memphis, 
      Tennessee, USA.
FAU - Rao, RadhaKrishna
AU  - Rao R
AD  - Department of Physiology, University of Tennessee Health Science Center, Memphis, 
      Tennessee, USA.
LA  - eng
GR  - R01 DK055532/DK/NIDDK NIH HHS/United States
GR  - R43 AI136551/AI/NIAID NIH HHS/United States
GR  - R56 DK055532/DK/NIDDK NIH HHS/United States
GR  - R43 AI096551/AI/NIAID NIH HHS/United States
GR  - R01 AA012307/AA/NIAAA NIH HHS/United States
PT  - Journal Article
DEP - 20180618
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
PMC - PMC6181630
OTO - NOTNLM
OT  - alcoholic hepatitis
OT  - barrier function
OT  - colon
OT  - cytokines
OT  - probiotics
COIS- This study was supported by U.S. National Institutes of Health Grants AA12307 
      (National Institute on Alcohol Abuse and Alcoholism) and DK55532 (National 
      Institute of Diabetes and Digestive and Kidney Diseases) to R.R. The authors 
      declare no conflicts of interest.
EDAT- 2018/06/19 06:00
MHDA- 2018/06/19 06:00
PMCR- 2019/11/01
CRDT- 2018/06/19 06:00
PHST- 2018/06/19 06:00 [entrez]
PHST- 2018/06/19 06:00 [pubmed]
PHST- 2018/06/19 06:00 [medline]
PHST- 2019/11/01 00:00 [pmc-release]
AID - FJ_201800351R [pii]
AID - 10.1096/fj.201800351R [doi]
PST - aheadofprint
SO  - FASEB J. 2018 Jun 18;32(11):fj201800351R. doi: 10.1096/fj.201800351R.