PMID- 29916168
OWN - NLM
STAT- MEDLINE
DCOM- 20190206
LR  - 20190215
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1774
DP  - 2018
TI  - Whole-Mount BrdU Staining with Fluorescence In Situ Hybridization in Planarians.
PG  - 423-434
LID - 10.1007/978-1-4939-7802-1_15 [doi]
AB  - Detection of cell proliferation based on the incorporation of 
      5'-bromo-2'-deoxyuridine (BrdU) has become a standard approach for studying stem 
      cell and progenitor cell populations in developing and adult tissue. In this 
      chapter, we describe three BrdU administration methods for planarians and a 
      staining protocol combining BrdU detection with whole-mount fluorescence in situ 
      hybridization (FISH). Collectively, these protocols enable the combined analysis 
      of BrdU-incorporation and endogenous gene expression, as for example during 
      lineage tracing applications.
FAU - Cheng, Li-Chun
AU  - Cheng LC
AD  - The Scripps Research Institute, La Jolla, CA, USA.
FAU - Alvarado, Alejandro Sanchez
AU  - Alvarado AS
AD  - Howard Hughes Medical Institute, Stowers Institute for Medical Research, Kansas 
      City, MO, USA. asa@stowers.org.
LA  - eng
GR  - HHMI/Howard Hughes Medical Institute/United States
GR  - R37 GM057260/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - Bromodeoxyuridine/*chemistry
MH  - Cell Proliferation/genetics
MH  - Gene Expression/genetics
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Planarians/*genetics
OTO - NOTNLM
OT  - BrdU
OT  - Cell proliferation
OT  - Fluorescence in situ hybridization
OT  - Immunostaining
OT  - Planarians
OT  - Whole-mount
EDAT- 2018/06/20 06:00
MHDA- 2019/02/07 06:00
CRDT- 2018/06/20 06:00
PHST- 2018/06/20 06:00 [entrez]
PHST- 2018/06/20 06:00 [pubmed]
PHST- 2019/02/07 06:00 [medline]
AID - 10.1007/978-1-4939-7802-1_15 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2018;1774:423-434. doi: 10.1007/978-1-4939-7802-1_15.