PMID- 29916307
OWN - NLM
STAT- Publisher
LR  - 20240227
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Linking)
DP  - 2018 Aug 15
TI  - Stromal Cell Derived Factor-1-Mediated Migration of Mesenchymal Stem Cells 
      Enhances Collagen Type II Expression in Intervertebral Disc.
LID - 10.1089/ten.TEA.2018.0131 [doi]
AB  - Intervertebral disc (IVD) degeneration is characterized by an unbalanced cell 
      catabolic/anabolic activity and cell death, resulting in the degradation of 
      extracellular matrix components and water loss. Repopulating the IVD with new 
      cells may help in recovering tissue homeostasis and reverting the degenerative 
      process. In this study the regenerative potential of a hyaluronan (HA)-based 
      chemoattractant delivery system able to recruit mesenchymal stem cells (MSCs) 
      seeded on the cartilaginous endplate (CEP) of IVD was explored. A HA delivery 
      system containing stromal cell derived factor-1 (SDF-1) (5 ng/muL) (HAPSDF5) was 
      injected in the cavity of nucleotomized bovine discs. Human MSCs (1 x 10(6)) were 
      seeded on the opposite CEP and allowed to migrate for up to 21 days. Migration of 
      fluorescently labelled MSCs from CEP toward the IVD was enhanced by HAPSDF5. 
      Likewise, an increase in collagen type II was detected at earlier time points, 
      whereas no effect on proteoglycan content within the nucleotomized IVDs was 
      found. MSCs produced an increased concentration of pro-catabolic factors, such as 
      interleukin (IL)-6, IL-8, and monocyte chemoattractant protein-1 (MCP-1). 
      Overall, this study demonstrates that HAPSDF5 increased MSC recruitment, while 
      the higher number of recruited cells partially contributed to accelerate matrix 
      remodeling in nucleotomized IVDs.
FAU - Leite Pereira, Catarina
AU  - Leite Pereira C
AD  - 1 Instituto de Investigacao e Inovacao em Saude (I3S), Universidade do Porto , 
      Porto, Portugal .
AD  - 2 Instituto de Engenharia Biomedica (INEB), Universidade do Porto , Porto, 
      Portugal .
FAU - Quelhas Teixeira, Graciosa
AU  - Quelhas Teixeira G
AD  - 1 Instituto de Investigacao e Inovacao em Saude (I3S), Universidade do Porto , 
      Porto, Portugal .
AD  - 2 Instituto de Engenharia Biomedica (INEB), Universidade do Porto , Porto, 
      Portugal .
FAU - Rita Ferreira, Joana
AU  - Rita Ferreira J
AD  - 1 Instituto de Investigacao e Inovacao em Saude (I3S), Universidade do Porto , 
      Porto, Portugal .
AD  - 2 Instituto de Engenharia Biomedica (INEB), Universidade do Porto , Porto, 
      Portugal .
AD  - 3 Instituto de Ciencias Biomedicas Abel Salazar (ICBAS), Universidade do Porto , 
      Porto, Portugal .
FAU - D'Este, Matteo
AU  - D'Este M
AD  - 4 AO Research Institute Davos, AO Foundation , Davos, Switzerland .
FAU - Eglin, David
AU  - Eglin D
AD  - 4 AO Research Institute Davos, AO Foundation , Davos, Switzerland .
FAU - Alini, Maulo
AU  - Alini M
AD  - 4 AO Research Institute Davos, AO Foundation , Davos, Switzerland .
FAU - Grad, Sibylle
AU  - Grad S
AD  - 4 AO Research Institute Davos, AO Foundation , Davos, Switzerland .
FAU - Barbosa, Mario Adolfo
AU  - Barbosa MA
AD  - 1 Instituto de Investigacao e Inovacao em Saude (I3S), Universidade do Porto , 
      Porto, Portugal .
AD  - 2 Instituto de Engenharia Biomedica (INEB), Universidade do Porto , Porto, 
      Portugal .
AD  - 3 Instituto de Ciencias Biomedicas Abel Salazar (ICBAS), Universidade do Porto , 
      Porto, Portugal .
FAU - Goncalves, Raquel Madeira
AU  - Goncalves RM
AD  - 1 Instituto de Investigacao e Inovacao em Saude (I3S), Universidade do Porto , 
      Porto, Portugal .
AD  - 2 Instituto de Engenharia Biomedica (INEB), Universidade do Porto , Porto, 
      Portugal .
LA  - eng
PT  - Journal Article
DEP - 20180815
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
OTO - NOTNLM
OT  - biomaterials
OT  - cell recruitment
OT  - intervertebral disc
OT  - regeneration
OT  - spine
OT  - stem cell migration
EDAT- 2018/06/20 06:00
MHDA- 2018/06/20 06:00
CRDT- 2018/06/20 06:00
PHST- 2018/06/20 06:00 [pubmed]
PHST- 2018/06/20 06:00 [medline]
PHST- 2018/06/20 06:00 [entrez]
AID - 10.1089/ten.TEA.2018.0131 [doi]
PST - aheadofprint
SO  - Tissue Eng Part A. 2018 Aug 15. doi: 10.1089/ten.TEA.2018.0131.