PMID- 29916443
OWN - NLM
STAT- MEDLINE
DCOM- 20181030
LR  - 20220318
IS  - 0972-9062 (Print)
IS  - 0972-9062 (Linking)
VI  - 55
IP  - 1
DP  - 2018 Jan-Mar
TI  - Identification of Leishmania species using N-acetylglucosamine-1-phosphate 
      transferase gene in a zoonotic cutaneous leishmaniasis focus of Iran.
PG  - 14-19
LID - 10.4103/0972-9062.234621 [doi]
AB  - BACKGROUND & OBJECTIVES: Ilam province is one of the oldest known endemic foci of 
      zoonotic cutaneous leishmani- asis (CL) in Iran; and the recent studies have 
      shown an increasing trend in the number of cases from the region. This study was 
      aimed to investigate the parasite species and genetic diversity of isolates 
      obtained from CL patients based on the N-acetylglucosamine-1-phosphate 
      transferase (nagt) gene. METHODS: Exudate materials were collected from the 
      swollen margin of the skin lesions of the patients suspected with CL who were 
      referred to health centers laboratory of Mehran, Dehloran, Ilam and Malekshahi 
      cities in the Ilam province. Demographic data were collected through a 
      questionnaire. Smears were stained and examined microscopically. In total, 62 
      parasitologically positive samples were subjected to PCR-RFLP of nagt gene for 
      identification of Leishmania species, in addition to genetic diversity 
      investigation. RESULTS: Nearly, half of the positive cases were referred from 
      Mehran followed by Dehloran City (40.4%). These included people from different 
      age groups (1 to 73 yr), with majority being male (66.1%). The common site of 
      lesions was hand (48.4%). Half of the patients had multiple lesions; most of them 
      were wet ulcerative type. A 1450-60 bp band of the nagt gene was amplified from 
      all the samples. Digestion patterns of acetyl-coenzyme A carboxylase 1 (ACC1) 
      enzyme were similar to what expected for Leishmania major. No difference was 
      observed at the nucleotide acid level or resulting amino acid in nine sequenced 
      samples on the basis of phylogenetic analyses. However, intra- species 
      differences (0.0015) were observed amongst the L. major isolates of present study 
      and the other parts of Iran. INTERPRETATION & CONCLUSION: The findings of this 
      study demonstrated that the main causative agent of CL in Ilam Province is L. 
      major, and there is no considerable heterogeneity among the L. major isolates. 
      Moreover, nagt gene proved to be an efficient marker for differentiating 
      Leishmania species. Further studies with more samples need to be carried out to 
      achieve a more comprehensive result on the genetic variation of L. major 
      isolates.
FAU - Saberi, Reza
AU  - Saberi R
AD  - Cellular and Molecular Biology Research Center; Department of Parasitology and 
      Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, 
      Tehran, Iran.
FAU - Moin-Vaziri, Vahideh
AU  - Moin-Vaziri V
AD  - Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti 
      University of Medical Sciences, Tehran, Iran.
FAU - Hajjaran, Homa
AU  - Hajjaran H
AD  - Department of Medical Parasitology and Mycology, School of Public Health, Tehran 
      University of Medical Sciences, Tehran, Iran.
FAU - Niyyati, Maryam
AU  - Niyyati M
AD  - Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti 
      University of Medical Sciences, Tehran, Iran.
FAU - Taghipour, Niloofar
AU  - Taghipour N
AD  - Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti 
      University of Medical Sciences, Tehran, Iran.
FAU - Kheirandish, Farnaz
AU  - Kheirandish F
AD  - Department of Medical Parasitology and Mycology, Lorestan University of Medical 
      Sciences, Khorramabad, Iran.
FAU - Abadi, Alireza
AU  - Abadi A
AD  - Department of Community and Health, Shahid Beheshti University of Medical 
      Sciences, Tehran, Iran.
LA  - eng
PT  - Journal Article
PL  - India
TA  - J Vector Borne Dis
JT  - Journal of vector borne diseases
JID - 101212761
RN  - 0 (DNA, Protozoan)
RN  - EC 2.7.8.- (Transferases (Other Substituted Phosphate Groups))
RN  - EC 2.7.8.15 (UDPacetylglucosamine-dolichyl-phosphate 
      acetylglucosamine-1-phosphate transferase)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Child
MH  - Child, Preschool
MH  - DNA, Protozoan/genetics
MH  - Female
MH  - *Genetic Variation
MH  - Humans
MH  - Infant
MH  - Iran/epidemiology
MH  - Leishmania major/classification/enzymology/*genetics/isolation & purification
MH  - Leishmaniasis, Cutaneous/*diagnosis/epidemiology/*parasitology
MH  - Male
MH  - Middle Aged
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - Sequence Analysis, DNA
MH  - Skin/parasitology/pathology
MH  - Transferases (Other Substituted Phosphate Groups)/*genetics
MH  - Young Adult
MH  - Zoonoses/parasitology
OTO - NOTNLM
OT  - Genetic diversity
OT  - Ilam
OT  - Iran
OT  - Leishmania major
OT  - gene
OT  - nagt
COIS- The authors declare that they don't have any significant competing financial, 
      professional or personal interests that might have influenced the results of the 
      study
EDAT- 2018/06/20 06:00
MHDA- 2018/10/31 06:00
CRDT- 2018/06/20 06:00
PHST- 2018/06/20 06:00 [entrez]
PHST- 2018/06/20 06:00 [pubmed]
PHST- 2018/10/31 06:00 [medline]
AID - JVectorBorneDis_2018_55_1_14_234621 [pii]
AID - 10.4103/0972-9062.234621 [doi]
PST - ppublish
SO  - J Vector Borne Dis. 2018 Jan-Mar;55(1):14-19. doi: 10.4103/0972-9062.234621.