PMID- 2991901
OWN - NLM
STAT- MEDLINE
DCOM- 19850906
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 82
IP  - 15
DP  - 1985 Aug
TI  - Differentiation-enhanced binding of the amino-terminal fragment of human 
      urokinase plasminogen activator to a specific receptor on U937 monocytes.
PG  - 4939-43
AB  - The purified amino-terminal fragment (ATF) of human urokinase plasminogen 
      activator (residues 1-135), which is not required for activation of plasminogen, 
      binds with high affinity to specific plasma membrane receptors on U937 monocytes. 
      Intact urokinase efficiently competes for 125I-labeled ATF binding; 50% 
      competition occurs with 1 nM urokinase. A large part of receptor-bound urokinase 
      remains on the cell surface for at least 2 hr at 37 degrees C. Differentiation of 
      U937 monocytes into macrophage-like cells specifically increases ATF binding 10- 
      to 20-fold. These results suggest an important role for urokinase in 
      monocyte/macrophage biology: the native enzyme binds to the cells with the 
      amino-terminal domain; the catalytic, carboxyl-terminal domain remains exposed on 
      the cell surface to stimulate localized proteolysis and facilitate cell 
      migration.
FAU - Stoppelli, M P
AU  - Stoppelli MP
FAU - Corti, A
AU  - Corti A
FAU - Soffientini, A
AU  - Soffientini A
FAU - Cassani, G
AU  - Cassani G
FAU - Blasi, F
AU  - Blasi F
FAU - Assoian, R K
AU  - Assoian RK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (PLAUR protein, human)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Urokinase Plasminogen Activator)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding, Competitive
MH  - Cell Differentiation/drug effects
MH  - Cell Membrane/metabolism
MH  - Humans
MH  - Macrophages/metabolism
MH  - Monocytes/*metabolism
MH  - Peptide Fragments/metabolism
MH  - Receptors, Cell Surface/*metabolism
MH  - Receptors, Urokinase Plasminogen Activator
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Urokinase-Type Plasminogen Activator/*metabolism
PMC - PMC390473
EDAT- 1985/08/01 00:00
MHDA- 1985/08/01 00:01
PMCR- 1986/02/01
CRDT- 1985/08/01 00:00
PHST- 1985/08/01 00:00 [pubmed]
PHST- 1985/08/01 00:01 [medline]
PHST- 1985/08/01 00:00 [entrez]
PHST- 1986/02/01 00:00 [pmc-release]
AID - 10.1073/pnas.82.15.4939 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1985 Aug;82(15):4939-43. doi: 10.1073/pnas.82.15.4939.