PMID- 29920038
OWN - NLM
STAT- MEDLINE
DCOM- 20180730
LR  - 20181202
IS  - 2095-4352 (Print)
VI  - 28
IP  - 5
DP  - 2016 May
TI  - [Effect of unfractionated heparin on the expression of heme oxygenase-1 in 
      intestinal mucosa of mice with sepsis].
PG  - 423-6
AB  - OBJECTIVE: To investigate the effect of unfractionated heparin (UFH) on the 
      expression of heme oxygenase-1 (HO-1) in intestinal mucosa of mice with sepsis. 
      METHODS: Thirty-six male C57BL/6J mice were randomly divided into sham group, 
      cecal ligation and puncture (CLP) group and UHF group, n =12 in each group. Model 
      of intestinal injury in sepsis was induced by CLP. In sham group, the mice were 
      exposed without ligation of cecum. In UFH group, the mice were treated 
      intravenously with 8 U of UFH via the tail vein half an hour before the operation 
      and 12 hours after the surgery respectively. Six mice in each group were randomly 
      chosen at 4 hours and 24 hours after operation to collect inferior vena venous 
      blood samples and terminalileum tissues. The serum levels of interleukins (IL-1 
      beta,IL-6),and tumor necrosis factor-alpha (TNF-alpha) were determined by enzyme linked 
      immunosorbent assay (ELISA).The serum level of D-lactate was determined by 
      colorimetry.Pathological changes of ileum tissue and Chiu score were observed 
      after hematoxylin eosin (HE) staining. The HO-1 expression was detected 
      immunohistochemically. RESULTS: In sham group, no significant changes in the 
      serum levels of IL-1 beta,IL-6,TNF-alpha and D-lactate were observed. Twenty-four hours 
      after the operation, the structure of intestinal mucosa was basically normal 
      without obvious pathology change and no HO-1 positive cells were found. The serum 
      levels of IL-1 beta,IL-6,TNF-alpha,and D-lactate in CLP group were gradually increased, 
      and they were significantly increased as compared with sham group [IL-1 beta 
      (ng/L):40.87+/-2.88 vs.22.60+/-2.05 at 4 hours,113.73+/-3.96 vs.22.07+/-2.74 at 24 
      hours;IL-6 (ng/L):63.89+/-3.26 vs.44.89+/-3.38 at 4 hours,176.56+/-5.45 vs.45.76+/-4.02 
      at 24 hours; TNF-alpha (ng/L):194.62+/- 14.13 vs.152.05+/-6.22 at 4 hours,599.62+/- 10.20 
      vs.155.90+/- 14.18 at 24 hours; D-lactate (mmol/L):0.24+/- 0.02 vs.0.19 +/- 0.01 at 4 
      hours,0.33 +/- 0.04 vs.0.20 +/- 0.02 at 24 hours, all P ＜ 0.05].Twenty-four hours 
      after the operation, edema and inflammation in ileal mucosa, intestinal villi 
      structural damage were observed, the Chiu score was significantly higher than 
      those in the sham group [4.5 (3.0-5.0) vs.0 (0-1.0),P ＜ 0.05],and a small amount 
      of HO-1 positive cells were localized in the intestinal mucosa. Compared with CLP 
      group, the serum levels of IL-1 beta,IL-6,TNF-alpha,and D-lactate of UFH group were 
      significantly decreased [IL-1 beta (ng/L):31.53 +/- 2.90 vs.40.87 +/- 2.88 at 4 
      hours,61.13 +/- 2.80 vs.113.73 +/- 3.96 at 24 hours;IL-6 (ng/L):51.16 +/- 5.68 vs.63.89 
      +/- 3.26 at 4 hours,81.16 +/- 4.54 vs.176.56 +/- 5.45 at 24 hours; TNF-alpha (ng/L):171.76+/- 
      5.60 vs.194.62+/- 14.13 at 4 hours,328.48 +/- 10.79 vs.599.62+/- 10.20 at 24 hours; 
      D-lactate (mmol/L):0.21 +/-0.01 vs.0.24+/-0.02 at 4 hours,0.24+/-0.02 vs.0.33+/-0.04 at 
      24 hours, all P ＜ 0.05]. Twenty-four hours after the operation, intestinal injury 
      was ameliorated, the Chiu score was significantly lower [1.5 (1.0-5.0) vs.4.5 
      (3.0-5.0),P ＜ 0.05],and HO-1 positive cells in the intestinal mucosa was 
      remarkably increased. CONCLUSION: UFH can enhance the expression of HO-1 in 
      intestinal mucosa, reduce the release of inflammatory factors, ameliorate the 
      intestinal inflammatory response, and thus play a protective role in intestinal 
      tissue in mice with sepsis.
FAU - Yin, Xiaohan
AU  - Yin X
FAU - Chen, Song
AU  - Chen S
FAU - Hu, Ziwei
AU  - Hu Z
FAU - Xiao, Feng
AU  - Xiao F
FAU - Lu, Siyu
AU  - Lu S
FAU - Ma, Xiaochun
AU  - Ma X
FAU - Luan, Zhenggang
AU  - Luan Z
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhonghua wei zhong bing ji jiu yi xue
JID - 101604552
RN  - 0 (Fibrinolytic Agents)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Membrane Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9005-49-6 (Heparin)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.14.14.18 (Hmox1 protein, mouse)
SB  - IM
MH  - Animals
MH  - Fibrinolytic Agents/*pharmacology
MH  - Heme Oxygenase-1/*metabolism
MH  - Heparin/*pharmacology
MH  - Inflammation
MH  - Interleukin-1beta
MH  - Intestinal Mucosa/metabolism
MH  - Intestines
MH  - Male
MH  - Membrane Proteins/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Sepsis/*metabolism
MH  - Tumor Necrosis Factor-alpha
EDAT- 2016/05/01 00:00
MHDA- 2018/07/31 06:00
CRDT- 2018/06/20 06:00
PHST- 2018/06/20 06:00 [entrez]
PHST- 2016/05/01 00:00 [pubmed]
PHST- 2018/07/31 06:00 [medline]
PST - ppublish
SO  - Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 2016 May;28(5):423-6.