PMID- 29920289
OWN - NLM
STAT- MEDLINE
DCOM- 20181023
LR  - 20181023
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 253
DP  - 2018 Jul 15
TI  - Galectin-3 inhibits replication of porcine reproductive and respiratory syndrome 
      virus by interacting with viral Nsp12 in vitro.
PG  - 87-91
LID - S0168-1702(18)30197-7 [pii]
LID - 10.1016/j.virusres.2018.06.006 [doi]
AB  - Porcine galectin-3 (GAL3) is a 29-kDa protein encoded by a single gene, LGALS3, 
      located on chromosome 1. Here, using a yeast two-hybrid screen of a cDNA library 
      from porcine alveolar macrophage cells (PAMs), we report for the first time that 
      GAL3 interacts with nonstructural protein 12 (Nsp12) of the porcine reproductive 
      and respiratory syndrome virus (PRRSV). Although extensive research has focused 
      on porcine reproductive and respiratory syndrome (PRRS), little is known about 
      the pathogen and host interactions involving individual nonstructural viral 
      proteins, especially Nsp12. Here, we showed that GAL3 interacted with viral Nsp12 
      following co-transfection of HEK293 cells with GAL3- and Nsp12-expressing 
      plasmids. Additionally, we observed that PPRSV infection led to reduced GAL3 
      levels during the late phase of infection in both MARC-145 cells and PAMs. 
      Importantly, GAL3 overexpression significantly suppressed the replication of both 
      type 1 and 2 PRRSV strains, whereas knockout of endogenous LGALS3 in MARC-145 
      cells significantly increased viral titer and expression of the nucleocapsid 
      protein. These results strongly support a direct inhibitory effect of GAL3 on 
      PRRSV replication, which might contribute to an overall antiviral effect. 
      Furthermore, our findings provide insights into the molecular basis of the role 
      Nsp12 plays in PRRSV pathogenesis.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Li, Liwei
AU  - Li L
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, PR China; Jiangsu Co-Innovation Center for the Prevention and 
      Control of Important Animal Infectious Disease and Zoonose, Yangzhou University, 
      Yangzhou 225009, PR China.
FAU - Zhou, Yanjun
AU  - Zhou Y
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, PR China; Jiangsu Co-Innovation Center for the Prevention and 
      Control of Important Animal Infectious Disease and Zoonose, Yangzhou University, 
      Yangzhou 225009, PR China.
FAU - Jiang, Yifeng
AU  - Jiang Y
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, PR China; Jiangsu Co-Innovation Center for the Prevention and 
      Control of Important Animal Infectious Disease and Zoonose, Yangzhou University, 
      Yangzhou 225009, PR China.
FAU - Gao, Fei
AU  - Gao F
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, PR China; Jiangsu Co-Innovation Center for the Prevention and 
      Control of Important Animal Infectious Disease and Zoonose, Yangzhou University, 
      Yangzhou 225009, PR China.
FAU - Shan, Tongling
AU  - Shan T
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, PR China; Jiangsu Co-Innovation Center for the Prevention and 
      Control of Important Animal Infectious Disease and Zoonose, Yangzhou University, 
      Yangzhou 225009, PR China.
FAU - Zhao, Kuan
AU  - Zhao K
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, PR China.
FAU - Zhang, Yujiao
AU  - Zhang Y
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, PR China.
FAU - Li, Lin
AU  - Li L
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, PR China.
FAU - Tong, Guangzhi
AU  - Tong G
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, PR China; Jiangsu Co-Innovation Center for the Prevention and 
      Control of Important Animal Infectious Disease and Zoonose, Yangzhou University, 
      Yangzhou 225009, PR China. Electronic address: gztong@shvri.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180618
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Galectin 3)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Animals
MH  - Galectin 3/genetics/*metabolism
MH  - Host-Pathogen Interactions
MH  - Porcine Reproductive and Respiratory Syndrome/genetics/*metabolism/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*physiology
MH  - Protein Binding
MH  - Swine
MH  - Viral Nonstructural Proteins/genetics/*metabolism
MH  - *Virus Replication
OTO - NOTNLM
OT  - Galectin-3
OT  - Nsp12
OT  - PRRSV
EDAT- 2018/06/20 06:00
MHDA- 2018/10/24 06:00
CRDT- 2018/06/20 06:00
PHST- 2018/04/02 00:00 [received]
PHST- 2018/06/05 00:00 [revised]
PHST- 2018/06/15 00:00 [accepted]
PHST- 2018/06/20 06:00 [pubmed]
PHST- 2018/10/24 06:00 [medline]
PHST- 2018/06/20 06:00 [entrez]
AID - S0168-1702(18)30197-7 [pii]
AID - 10.1016/j.virusres.2018.06.006 [doi]
PST - ppublish
SO  - Virus Res. 2018 Jul 15;253:87-91. doi: 10.1016/j.virusres.2018.06.006. Epub 2018 
      Jun 18.