PMID- 29920365
OWN - NLM
STAT- MEDLINE
DCOM- 20180820
LR  - 20180820
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 673
DP  - 2018 Oct 5
TI  - tdrd1 is a germline-specific and sexually dimorphically expressed gene in 
      Paralichthys olivaceus.
PG  - 61-69
LID - S0378-1119(18)30699-1 [pii]
LID - 10.1016/j.gene.2018.06.043 [doi]
AB  - Tudor domain containing protein 1 (tdrd1) is a member of the Tudor family and has 
      shown essential functions during embryogenesis and gametogenesis. In this study, 
      we cloned the full length cDNA of Paralichthys olivaceus tdrd1 (Potdrd1). PoTDRD1 
      is a multidomain protein with an N-terminal MYND zinc finger domain, followed by 
      four tandem extended Tudor domains. Sequence comparison, genomic structure, 
      phylogenetic analyses and synteny analyses showed that Potdrd1 was homologous to 
      those of other teleosts. In adult individuals, the expression of Potdrd1 was 
      higher in testis than in ovary, demonstrating a sexually dimorphic gene 
      expression pattern. In situ hybridization (ISH) showed that Potdrd1 mRNA was 
      detected in oogonia and oocytes of ovary as well as in spermatogonia and 
      spermatocytes of testis. In juveniles during gonad differentiation its expression 
      level increased rapidly from 30 dph to 100 dph and showed obvious sexual 
      dimorphism that was in accordance with the expression of anti-Mullerian hormone 
      (amh). Potdrd1 mRNA was consistently detected during embryogenesis, and its level 
      was higher from unfertilzed eggs to the blastula stage and subsequently decreased 
      until hatching. When chimeric RNA containing green fluorescent protein (GFP) and 
      3' untranslated regions (UTR) of Potdrd1 was microinjected into zebrafish 
      fertilized eggs, the green fluorescence could be visualized only in putative 
      PGCs. These results indicated that Potdrd1 is a germline specific and sexually 
      dimorphic factor that potentially functionate in germline development and 
      gametogenesis in Japanese flounder.
CI  - Copyright (c) 2018. Published by Elsevier B.V.
FAU - Zhao, Jun
AU  - Zhao J
AD  - Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean 
      University of China, 266003 Qingdao, Shandong, China.
FAU - Wang, Bo
AU  - Wang B
AD  - Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean 
      University of China, 266003 Qingdao, Shandong, China.
FAU - Yu, Haiyang
AU  - Yu H
AD  - Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean 
      University of China, 266003 Qingdao, Shandong, China.
FAU - Wang, Yujue
AU  - Wang Y
AD  - Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean 
      University of China, 266003 Qingdao, Shandong, China.
FAU - Liu, Xiaobing
AU  - Liu X
AD  - Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean 
      University of China, 266003 Qingdao, Shandong, China.
FAU - Zhang, Quanqi
AU  - Zhang Q
AD  - Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean 
      University of China, 266003 Qingdao, Shandong, China; Laboratory for Marine 
      Fisheries Science and Food Production Processes, Qingdao National Laboratory for 
      Marine Science and Technology, China. Electronic address: qzhang@ouc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20180618
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Carrier Proteins)
RN  - 0 (Fish Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Animals
MH  - Carrier Proteins/*genetics/metabolism
MH  - Cloning, Molecular
MH  - Female
MH  - Fish Proteins/*genetics/metabolism
MH  - Flounder/*genetics
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Developmental
MH  - Germ Cells/cytology
MH  - *Germ-Line Mutation
MH  - Green Fluorescent Proteins/metabolism
MH  - In Situ Hybridization
MH  - Male
MH  - Oocytes/cytology/metabolism
MH  - Oogonia/metabolism
MH  - Ovary/metabolism
MH  - Phylogeny
MH  - Protein Domains
MH  - Sex Characteristics
MH  - Spermatocytes/metabolism
MH  - Spermatogonia/metabolism
MH  - Testis/metabolism
OTO - NOTNLM
OT  - Germline specific
OT  - Gonad differentiation
OT  - Potdrd1
OT  - Visualized PGCs
EDAT- 2018/06/20 06:00
MHDA- 2018/08/21 06:00
CRDT- 2018/06/20 06:00
PHST- 2018/03/21 00:00 [received]
PHST- 2018/05/29 00:00 [revised]
PHST- 2018/06/14 00:00 [accepted]
PHST- 2018/06/20 06:00 [pubmed]
PHST- 2018/08/21 06:00 [medline]
PHST- 2018/06/20 06:00 [entrez]
AID - S0378-1119(18)30699-1 [pii]
AID - 10.1016/j.gene.2018.06.043 [doi]
PST - ppublish
SO  - Gene. 2018 Oct 5;673:61-69. doi: 10.1016/j.gene.2018.06.043. Epub 2018 Jun 18.