PMID- 29920546 OWN - NLM STAT- MEDLINE DCOM- 20181226 LR - 20221207 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 13 IP - 6 DP - 2018 TI - Antidiabetic effect of Euterpe oleracea Mart. (acai) extract and exercise training on high-fat diet and streptozotocin-induced diabetic rats: A positive interaction. PG - e0199207 LID - 10.1371/journal.pone.0199207 [doi] LID - e0199207 AB - A growing body of evidence suggests a protective role of polyphenols and exercise training on the disorders of type 2 diabetes mellitus (T2DM). We aimed to assess the effect of the acai seed extract (ASE) associated with exercise training on diabetic complications induced by high-fat (HF) diet plus streptozotocin (STZ) in rats. Type 2 diabetes was induced by feeding rats with HF diet (55% fat) for 5 weeks and a single dose of STZ (35 mg/kg i.p.). Control (C) and Diabetic (D) animals were subdivided into four groups each: Sedentary, Training, ASE Sedentary, and ASE Training. ASE (200 mg/kg/day) was administered by gavage and the exercise training was performed on a treadmill (30min/day; 5 days/week) for 4 weeks after the diabetes induction. In type 2 diabetic rats, the treatment with ASE reduced blood glucose, insulin resistance, leptin and IL-6 levels, lipid profile, and vascular dysfunction. ASE increased the expression of insulin signaling proteins in skeletal muscle and adipose tissue and plasma GLP-1 levels. ASE associated with exercise training potentiated the reduction of glycemia by decreasing TNF-alpha levels, increasing pAKT and adiponectin expressions in adipose tissue, and IR and pAMPK expressions in skeletal muscle of type 2 diabetic rats. In conclusion, ASE treatment has an antidiabetic effect in type 2 diabetic rats by activating the insulin-signaling pathway in muscle and adipose tissue, increasing GLP-1 levels, and an anti-inflammatory action. Exercise training potentiates the glucose-lowering effect of ASE by activating adiponectin-AMPK pathway and increasing IR expression. FAU - de Bem, Graziele Freitas AU - de Bem GF AD - Department of Pharmacology, Institute of Biology, Rio de Janeiro State University, Rio de Janeiro, RJ, Brazil. FAU - Costa, Cristiane Aguiar AU - Costa CA AD - Department of Pharmacology, Institute of Biology, Rio de Janeiro State University, Rio de Janeiro, RJ, Brazil. FAU - Santos, Izabelle Barcellos AU - Santos IB AD - Department of Pharmacology, Institute of Biology, Rio de Janeiro State University, Rio de Janeiro, RJ, Brazil. FAU - Cristino Cordeiro, Viviane da Silva AU - Cristino Cordeiro VDS AD - Department of Pharmacology, Institute of Biology, Rio de Janeiro State University, Rio de Janeiro, RJ, Brazil. FAU - de Carvalho, Lenize Costa Reis Marins AU - de Carvalho LCRM AD - Department of Pharmacology, Institute of Biology, Rio de Janeiro State University, Rio de Janeiro, RJ, Brazil. FAU - de Souza, Marcelo Augusto Vieira AU - de Souza MAV AD - Department of Chemical Processes, Institute of Chemistry, Rio de Janeiro State University, Rio de Janeiro, RJ, Brazil. FAU - Soares, Ricardo de Andrade AU - Soares RA AD - Department of Pharmacology, Institute of Biology, Rio de Janeiro State University, Rio de Janeiro, RJ, Brazil. FAU - Sousa, Pergentino Jose da Cunha AU - Sousa PJDC AD - Department of Pharmacy, Federal University of Para, Belem, PA, Brazil. FAU - Ognibene, Dayane Teixeira AU - Ognibene DT AD - Department of Pharmacology, Institute of Biology, Rio de Janeiro State University, Rio de Janeiro, RJ, Brazil. FAU - Resende, Angela Castro AU - Resende AC AUID- ORCID: 0000-0002-7465-6171 AD - Department of Pharmacology, Institute of Biology, Rio de Janeiro State University, Rio de Janeiro, RJ, Brazil. FAU - de Moura, Roberto Soares AU - de Moura RS AD - Department of Pharmacology, Institute of Biology, Rio de Janeiro State University, Rio de Janeiro, RJ, Brazil. LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180619 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 0 (Blood Glucose) RN - 0 (Glycated Hemoglobin A) RN - 0 (Hypoglycemic Agents) RN - 0 (Il6 protein, rat) RN - 0 (Insulin) RN - 0 (Interleukin-6) RN - 0 (Leptin) RN - 0 (Lipids) RN - 0 (Plant Extracts) RN - 89750-14-1 (Glucagon-Like Peptide 1) SB - IM MH - Adipose Tissue/drug effects/metabolism MH - Animals MH - Blood Glucose/analysis MH - Combined Modality Therapy MH - Diabetes Mellitus, Experimental/blood/*drug therapy/therapy MH - Diet, High-Fat MH - Drug Evaluation, Preclinical MH - *Euterpe MH - Glucagon-Like Peptide 1/blood MH - Glycated Hemoglobin/analysis MH - Hypoglycemic Agents/isolation & purification/pharmacology/*therapeutic use MH - Insulin/blood MH - Insulin Resistance MH - Interleukin-6/blood MH - Leptin/blood MH - Lipids/blood MH - Male MH - Muscle, Skeletal/drug effects/metabolism MH - *Physical Conditioning, Animal MH - *Phytotherapy MH - Plant Extracts/pharmacology/*therapeutic use MH - Random Allocation MH - Rats MH - Rats, Wistar MH - Seeds/chemistry PMC - PMC6007924 COIS- We have read the journal's policy and the authors of this manuscript have the following competing interests: Roberto Soares de Moura is the inventor of the following patent application: Process to obtain decoctions of skins and stone of the fruit of Euterpe oleracea (Acai); process to obtain a hydro-alcoholic extract from the decoctions; process to obtain a lyophilized and/or spray dried from the hydro-alcoholic extracts; process to obtain pharmaceutical preparations containing the lyophilized and/or spay dried, and therapeutic indications of the preparations as vasodilators and on the prevention and treatment of vasospastic ischemical syndromes and arterial hypertension (PI/0604281-3). The other authors have declared that no competing interests exist. This does not alter our adherence to PLOS ONE policies on sharing data and materials. EDAT- 2018/06/20 06:00 MHDA- 2018/12/27 06:00 PMCR- 2018/06/19 CRDT- 2018/06/20 06:00 PHST- 2018/02/23 00:00 [received] PHST- 2018/06/04 00:00 [accepted] PHST- 2018/06/20 06:00 [entrez] PHST- 2018/06/20 06:00 [pubmed] PHST- 2018/12/27 06:00 [medline] PHST- 2018/06/19 00:00 [pmc-release] AID - PONE-D-18-05962 [pii] AID - 10.1371/journal.pone.0199207 [doi] PST - epublish SO - PLoS One. 2018 Jun 19;13(6):e0199207. doi: 10.1371/journal.pone.0199207. eCollection 2018.