PMID- 2992157
OWN - NLM
STAT- MEDLINE
DCOM- 19850916
LR  - 20190714
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 145
IP  - 2
DP  - 1985 Sep
TI  - Molecular cloning of integrated caprine arthritis-encephalitis virus.
PG  - 340-5
AB  - A full-length DNA clone of the exogenous retrovirus, caprine 
      arthritis-encephalitis virus (CAEV), was isolated from high molecular weight DNA 
      of CAEV-infected Himalayan tahr ovary cells. Although other restriction maps of 
      CAEV have been published, this is the first time that the proviral DNA has been 
      cloned. The restriction enzyme map of the clone was determined and found to be 
      identical to that of unintegrated linear CAEV DNA except for the presence of 
      cellular flanking sequences. These findings establish that lentiviruses are able 
      to integrate within the infected host cellular genome. The cloned CAEV genome was 
      shown to contain terminal repeats of approximately 450 base pairs in length, and 
      its restriction enzyme map was oriented with respect to the direction of viral 
      RNA transcription. When the cloned CAEV DNA was used as a molecular probe, it 
      failed to detect related proviral sequences in the genomes of a variety of 
      vertebrate species, including the goat, sheep, horse, mouse, and man. When CAEV 
      DNA was hybridized under relaxed conditions to a variety of cloned DNAs, 
      representing different oncoviral genera, homology to mouse mammary tumor virus 
      (MMTV) was observed, while no homology to avian type C or mammalian type A, C, 
      and D retroviruses was detected. This homology was localized to a region in MMTV 
      corresponding to the 3' end of the gag gene and the 5' end of the pol gene.
FAU - Yaniv, A
AU  - Yaniv A
FAU - Dahlberg, J E
AU  - Dahlberg JE
FAU - Tronick, S R
AU  - Tronick SR
FAU - Chiu, I M
AU  - Chiu IM
FAU - Aaronson, S A
AU  - Aaronson SA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - EC 3.1.21.- (DNA Restriction Enzymes)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - *Cloning, Molecular
MH  - DNA Restriction Enzymes
MH  - Female
MH  - *Genes, Viral
MH  - Goats
MH  - Humans
MH  - Nucleic Acid Hybridization
MH  - Ovary
MH  - Retroviridae/*genetics
MH  - Species Specificity
EDAT- 1985/09/01 00:00
MHDA- 1985/09/01 00:01
CRDT- 1985/09/01 00:00
PHST- 1985/09/01 00:00 [pubmed]
PHST- 1985/09/01 00:01 [medline]
PHST- 1985/09/01 00:00 [entrez]
AID - 10.1016/0042-6822(85)90169-2 [doi]
PST - ppublish
SO  - Virology. 1985 Sep;145(2):340-5. doi: 10.1016/0042-6822(85)90169-2.