PMID- 29922436
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220318
IS  - 2008-3866 (Print)
IS  - 2008-3874 (Electronic)
IS  - 2008-3866 (Linking)
VI  - 21
IP  - 5
DP  - 2018 May
TI  - The role of nanoliposome bilayer composition containing soluble leishmania 
      antigen on maturation and activation of dendritic cells.
PG  - 536-545
LID - 10.22038/IJBMS.2018.25976.6391 [doi]
AB  - OBJECTIVES: Dendritic cells (DCs) play a critical role in activation of T cell 
      responses. Induction of type1 T helper (Th(1)) immune response is essential to 
      generate protective immunity against cutaneous leishmaniasis. The intrinsic 
      tendency of liposomes to have interaction with antigen-presenting cells is the 
      main rationale to utilize liposomes as antigen carriers. In the present study, 
      the effect of lipid phase transition temperature on DCs maturation and liposome 
      uptake by murine bone marrow derived dendritic cells and human monocyte derived 
      dendritic cells was investigated. MATERIALS AND METHODS: Two cationic liposomal 
      formulations consisting of DOTAP and DSPC/DOTAP were prepared and contained 
      soluble leishmania antigen. Liposomes were incubated with immature or mature DCs 
      derived from bone marrow (BMDCs) of C57BL/6 (which are resistant to cutaneous 
      leishmaniasis), BALB/c mice (susceptible to cutaneous leishmaniasis) or DCs 
      derived from human monocytes (MoDCs). The expression of DCs co-stimulatory 
      markers and liposomal uptake were evaluated by flow cytometry method. RESULTS: 
      DCs which were encountered to liposomes consisting of DSPC showed significantly 
      more expression of co-stimulatory molecules in cells from both human and C57BL/6 
      mice but not in cells from BALB/c mice. CONCLUSION: It is concluded that cationic 
      liposomes consisting of DSPC are an effective adjuvant for antigen delivery in 
      case of MoDCs and BMDCs from C57BL/6 mice. Moreover, DCs from different origins 
      act differently in uptake of liposomes.
FAU - Saremi, Sara Shokooh
AU  - Saremi SS
AD  - Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
AD  - Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
FAU - Shahryari, Maryam
AU  - Shahryari M
AD  - Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
AD  - Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
FAU - Ghoorchian, Razieh
AU  - Ghoorchian R
AD  - Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
FAU - Eshaghian, Hamed
AU  - Eshaghian H
AD  - Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
FAU - Jalali, Seyed Amir
AU  - Jalali SA
AD  - Department of Immunology, School of Medicine, Shahid Beheshti University of 
      Medical Sciences, Tehran, Iran.
FAU - Nikpoor, Amin Reza
AU  - Nikpoor AR
AD  - Department of Immunology, School of Medicine, Mashhad University of Medical 
      Sciences, Mashhad, Iran.
FAU - Jafari, Mahmoud Reza
AU  - Jafari MR
AD  - Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
AD  - Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
AD  - Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
FAU - Badiee, Ali
AU  - Badiee A
AD  - Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
AD  - Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Basic Med Sci
JT  - Iranian journal of basic medical sciences
JID - 101517966
PMC - PMC6000213
OTO - NOTNLM
OT  - Cationic liposomes
OT  - Dendritic cells
OT  - Immune response
OT  - Leishmaniasis
OT  - Phase transition - temperature
OT  - Th1
EDAT- 2018/06/21 06:00
MHDA- 2018/06/21 06:01
PMCR- 2018/05/01
CRDT- 2018/06/21 06:00
PHST- 2018/06/21 06:00 [entrez]
PHST- 2018/06/21 06:00 [pubmed]
PHST- 2018/06/21 06:01 [medline]
PHST- 2018/05/01 00:00 [pmc-release]
AID - IJBMS-21-536 [pii]
AID - 10.22038/IJBMS.2018.25976.6391 [doi]
PST - ppublish
SO  - Iran J Basic Med Sci. 2018 May;21(5):536-545. doi: 
      10.22038/IJBMS.2018.25976.6391.