PMID- 29926658
OWN - NLM
STAT- MEDLINE
DCOM- 20190104
LR  - 20231213
IS  - 1000-6834 (Print)
IS  - 1000-6834 (Linking)
VI  - 34
IP  - 1
DP  - 2018 Jan 8
TI  - [Effects of rosuvastatin in homocysteine induced mouse vascular smooth muscle 
      cell dedifferentiation and endoplasmic reticulum stress and its mechanisms].
PG  - 43-48
LID - 10.12047/j.cjap.5489.2018.012 [doi]
AB  - OBJECTIVE: To investigate the effect of rosuvastatin on homocysteine (Hcy) 
      induced mousevascular smooth muscle cells(VSMCs) dedifferentiation and 
      endoplasmic reticulum stress(ERS). METHODS: VSMCs were co-cultured with Hcy and 
      different concentration of rosuvastatin (0.1, 1.0 and 10 mumol/L). Cytoskeleton 
      remodeling, VSMCs phenotype markers (smooth muscle actin-alpha, calponin and 
      osteopontin) and ERS marker mRNAs (Herpud1, XBP1s and GRP78) were detected at 
      predicted time. Tunicamycin was used to induce, respectively 
      4-phenylbutyrate(4-PBA) inhibition, ERS in VSMCs and cellular migration, 
      proliferation and expression of phenotype proteins were analyzed. Mammalian 
      target of rapamycin(mTOR)-P70S6 kinase (P70S6K) signaling agonist phosphatidic 
      acid and inhibitor rapamycin were used in Rsv treated VSMCs. And then mTOR 
      signaling and ERS associated mRNAs were detected. RESULTS: Compared with Hcy 
      group, Hcy+ Rsv group (1.0 and 10 mumol/L) showed enhanced alpha-SMA and calponin 
      expression (P<0.01), suppressed ERS mRNA levels (P<0.01) and promoted polarity of 
      cytoskeleton. Compared with Hcy group, Hcy+Rsv group and Hcy+4-PBA group showed 
      suppressed proliferation, migration and enhanced contractile protein expression 
      (P<0.01); while tunicamycin could reverse the effect of Rsv on Hcy treated cells. 
      Furthermore, alleviated mTOR-P70S6K phosphorylation and ERS (P<0.01)were observed 
      in Hcy+Rsv group and Hcy+rapamycin group, compared with Hcy group; while 
      phosphatidic acid inhibited the effect of Rsv on mTOR signaling activation and 
      ERS mRNA levels (P<0.01). CONCLUSIONS: Rosuvastatin could inhibit Hcy induced 
      VSMCs dedifferentiation via suppressing ERS, which might be regulated by 
      mTOR-P70S6K signaling.
FAU - Zhou, Chang-Zuan
AU  - Zhou CZ
AD  - Department of Cardiology, Shaoxing People's Hospital, Shaoxing 312000.
AD  - Department of Cardiology, Wenzhou Hospital of Integrated Traditional Chinese and 
      Western Medicine, Wenzhou 32500, China.
FAU - Pan, Sun-Lei
AU  - Pan SL
AD  - Department of Cardiology, Shaoxing People's Hospital, Shaoxing 312000.
FAU - Lin, Hui
AU  - Lin H
AD  - Department of Cardiology, Shaoxing People's Hospital, Shaoxing 312000.
FAU - Meng, Li-Ping
AU  - Meng LP
AD  - Department of Cardiology, Shaoxing People's Hospital, Shaoxing 312000.
FAU - Ji, Zheng
AU  - Ji Z
AD  - Department of Cardiology, Shaoxing People's Hospital, Shaoxing 312000.
FAU - Chi, Ju-Fang
AU  - Chi JF
AD  - Department of Cardiology, Shaoxing People's Hospital, Shaoxing 312000.
FAU - Guo, Hang-Yuan
AU  - Guo HY
AD  - Department of Cardiology, Shaoxing People's Hospital, Shaoxing 312000.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Ying Yong Sheng Li Xue Za Zhi
JT  - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = 
      Chinese journal of applied physiology
JID - 9426407
RN  - 0 (Actins)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Endoplasmic Reticulum Chaperone BiP)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Herpud1 protein, mouse)
RN  - 0 (Hspa5 protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (X-Box Binding Protein 1)
RN  - 0 (Xbp1 protein, mouse)
RN  - 0 (alpha-smooth muscle actin, mouse)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 83MVU38M7Q (Rosuvastatin Calcium)
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Calcium-Binding Proteins/metabolism
MH  - Cell Dedifferentiation/*drug effects
MH  - Cells, Cultured
MH  - Endoplasmic Reticulum Chaperone BiP
MH  - Endoplasmic Reticulum Stress/*drug effects
MH  - Heat-Shock Proteins/metabolism
MH  - Homocysteine
MH  - Membrane Proteins/metabolism
MH  - Mice
MH  - Microfilament Proteins/metabolism
MH  - Muscle, Smooth, Vascular/*cytology
MH  - Myocytes, Smooth Muscle/cytology/*drug effects
MH  - Ribosomal Protein S6 Kinases, 70-kDa/metabolism
MH  - Rosuvastatin Calcium/*pharmacology
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - X-Box Binding Protein 1/metabolism
MH  - Calponins
OTO - NOTNLM
OT  - endoplasmic reticulum stress
OT  - homocysteine
OT  - mouse
OT  - phenotype dedifferentiation
OT  - rosuvastatin
OT  - vascular smooth muscle cell
EDAT- 2018/06/22 06:00
MHDA- 2019/01/05 06:00
CRDT- 2018/06/22 06:00
PHST- 2018/06/22 06:00 [entrez]
PHST- 2018/06/22 06:00 [pubmed]
PHST- 2019/01/05 06:00 [medline]
AID - 10.12047/j.cjap.5489.2018.012 [doi]
PST - ppublish
SO  - Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2018 Jan 8;34(1):43-48. doi: 
      10.12047/j.cjap.5489.2018.012.