PMID- 2992867
OWN - NLM
STAT- MEDLINE
DCOM- 19851022
LR  - 20190903
IS  - 0732-8893 (Print)
IS  - 0732-8893 (Linking)
VI  - 3
IP  - 5
DP  - 1985 Sep
TI  - A simple method to detect intrathecal production of specific antimeasles 
      antibodies in cerebrospinal fluid during subacute sclerosing panencephalitis.
PG  - 411-7
AB  - The intrathecal production of antimeasles antibodies was studied using the 
      enzyme-linked immunosorbent assay in eight specimens of serum and cerebrospinal 
      fluid (CSF) from patients with clinical signs of subacute sclerosing 
      panencephalitis (SSPE). The test was performed using a 1:5 dilution of CSF and a 
      1:2000 dilution of serum (ratio 1:400) in order to nullify the physiological 
      gradient of immunoglobulins across the blood brain barrier (BBB). This procedure 
      allowed a rapid and accurate assessment of the synthesis of specific 
      immunoglobulins in the CSF and a good evaluation of the permeability of the BBB. 
      A diagnosis of SSPE was provided in five out of eight patients with clinical 
      signs of the disease. Clinical follow-up confirmed the diagnosis of SSPE in the 
      group of patients with clear evidence of intrathecal synthesis of antimeasles 
      antibodies.
FAU - Melioli, G
AU  - Melioli G
FAU - Pedulla, D
AU  - Pedulla D
FAU - Merli, A L
AU  - Merli AL
FAU - Arata, L
AU  - Arata L
FAU - Leonardi, A
AU  - Leonardi A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Diagn Microbiol Infect Dis
JT  - Diagnostic microbiology and infectious disease
JID - 8305899
RN  - 0 (Antibodies, Viral)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antibodies, Viral/biosynthesis/*cerebrospinal fluid/immunology
MH  - Blood-Brain Barrier
MH  - Child
MH  - Cytomegalovirus/immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Herpesvirus 3, Human/immunology
MH  - Humans
MH  - Immunoglobulin G/*cerebrospinal fluid
MH  - Immunoglobulin M/*cerebrospinal fluid
MH  - Male
MH  - Measles virus/*immunology
MH  - Mumps virus/immunology
MH  - Rubella virus/immunology
MH  - Serologic Tests
MH  - Simplexvirus/immunology
MH  - Subacute Sclerosing Panencephalitis/*cerebrospinal fluid/immunology
EDAT- 1985/09/01 00:00
MHDA- 1985/09/01 00:01
CRDT- 1985/09/01 00:00
PHST- 1985/09/01 00:00 [pubmed]
PHST- 1985/09/01 00:01 [medline]
PHST- 1985/09/01 00:00 [entrez]
AID - 0732-8893(85)90080-X [pii]
AID - 10.1016/0732-8893(85)90080-x [doi]
PST - ppublish
SO  - Diagn Microbiol Infect Dis. 1985 Sep;3(5):411-7. doi: 
      10.1016/0732-8893(85)90080-x.