PMID- 29929057
OWN - NLM
STAT- MEDLINE
DCOM- 20190919
LR  - 20190919
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 93
DP  - 2018 Sep
TI  - Serotype a of Aggregatibacter actinomycetemcomitans down-regulates the increased 
      serotype b-induced cytokine and chemokine production in dendritic cells.
PG  - 155-162
LID - S0003-9969(18)30240-1 [pii]
LID - 10.1016/j.archoralbio.2018.06.010 [doi]
AB  - OBJECTIVE: In Aggregatibacter actinomycetemcomitans, different serotypes have 
      been described based on LPS antigenicity. Mixed infection with the different A. 
      actinomycetemcomitans serotypes is frequent in periodontitis patients; 
      accordingly, the role of this bacterial species in the pathogenesis of 
      periodontitis may differ depending whether patients or periodontal lesions 
      harbour one or more of the A. actinomycetemcomitans serotypes. We hypothesized 
      that different combinations of these serotypes could be associated with distinct 
      host responses and hence different inflammatory patterns. This investigation was 
      aimed to assess whether the increased immuno-stimulatory potential attributed to 
      the serotype b of A. actinomycetemcomitans on immune cells is able to be modified 
      during co-infection with other A. actinomycetemcomitans serotypes. METHODS: 
      Dendritic cells (DCs) were obtained from healthy subjects and stimulated with the 
      different A. actinomycetemcomitans serotypes or their purified LPS using the 
      following stimulatory conditions: serotype a, b, or c, and the combinations a+b, 
      a+c, b+c, or a+b+c. The cytokine, CCR, and CCL levels were quantified by qPCR and 
      ELISA. RESULTS: Higher levels of cytokines, CCRs, and CCLs were induced when DCs 
      were stimulated with the serotype b of A. actinomycetemcomitans compared with the 
      same cells stimulated with the other serotypes. When DCs were co-infected, these 
      levels decreased in comparison with the serotype b-stimulation alone, in 
      particular when the serotype a was present in the mixed infection. CONCLUSIONS: 
      The increased immuno-stimulatory potential attributed to the serotype b was 
      modified when DCs were co-infected with other A. actinomycetemcomitans serotypes, 
      in particular, when the serotype a was present, the DC response diminished.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Rojas, Leticia
AU  - Rojas L
AD  - Periodontal Biology Laboratory, Faculty of Dentistry, Universidad de Chile, 
      Santiago, Chile.
FAU - Melgar-Rodriguez, Samanta
AU  - Melgar-Rodriguez S
AD  - Periodontal Biology Laboratory, Faculty of Dentistry, Universidad de Chile, 
      Santiago, Chile.
FAU - Diaz-Zuniga, Jaime
AU  - Diaz-Zuniga J
AD  - Periodontal Biology Laboratory, Faculty of Dentistry, Universidad de Chile, 
      Santiago, Chile.
FAU - Alvarez, Carla
AU  - Alvarez C
AD  - Periodontal Biology Laboratory, Faculty of Dentistry, Universidad de Chile, 
      Santiago, Chile.
FAU - Monasterio, Gustavo
AU  - Monasterio G
AD  - Periodontal Biology Laboratory, Faculty of Dentistry, Universidad de Chile, 
      Santiago, Chile.
FAU - Rojas, Carolina
AU  - Rojas C
AD  - Periodontal Biology Laboratory, Faculty of Dentistry, Universidad de Chile, 
      Santiago, Chile.
FAU - Carvajal, Paola
AU  - Carvajal P
AD  - Department of Conservative Dentistry, Faculty of Dentistry, Universidad de Chile, 
      Santiago, Chile.
FAU - Vernal, Rolando
AU  - Vernal R
AD  - Periodontal Biology Laboratory, Faculty of Dentistry, Universidad de Chile, 
      Santiago, Chile; Dentistry Unit, Faculty of Health Sciences, Universidad Autonoma 
      de Chile, Santiago, Chile. Electronic address: rvernal@uchile.cl.
LA  - eng
PT  - Journal Article
DEP - 20180612
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Adult
MH  - Aggregatibacter actinomycetemcomitans/*immunology
MH  - Chemokines/immunology
MH  - Cytokines/*immunology
MH  - Dendritic Cells/*immunology
MH  - Down-Regulation
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Lipopolysaccharides
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Serogroup
OTO - NOTNLM
OT  - A. actinomycetemcomitans
OT  - Chemokine receptors
OT  - Chemokines
OT  - Cytokines
OT  - Serotypes
EDAT- 2018/06/22 06:00
MHDA- 2019/09/20 06:00
CRDT- 2018/06/22 06:00
PHST- 2017/09/09 00:00 [received]
PHST- 2018/06/04 00:00 [revised]
PHST- 2018/06/09 00:00 [accepted]
PHST- 2018/06/22 06:00 [pubmed]
PHST- 2019/09/20 06:00 [medline]
PHST- 2018/06/22 06:00 [entrez]
AID - S0003-9969(18)30240-1 [pii]
AID - 10.1016/j.archoralbio.2018.06.010 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2018 Sep;93:155-162. doi: 10.1016/j.archoralbio.2018.06.010. Epub 
      2018 Jun 12.