PMID- 29932217
OWN - NLM
STAT- MEDLINE
DCOM- 20190408
LR  - 20190408
IS  - 1097-0223 (Electronic)
IS  - 0197-3851 (Linking)
VI  - 38
IP  - 9
DP  - 2018 Aug
TI  - MicroRNA 200b is upregulated in the lungs of fetal rabbits with surgically 
      induced diaphragmatic hernia.
PG  - 645-653
LID - 10.1002/pd.5318 [doi]
AB  - OBJECTIVE: Profiling of miR-200b expression and its targets (transforming growth 
      factor [TGF]-beta2 and ZEB2) in the surgical rabbit congenital diaphragmatic hernia 
      (DH) model before and after tracheal occlusion (TO). METHODS: Thirty-eight 
      timed-pregnant rabbits had left DH creation on gestational day (GD) 23. On GD28, 
      17 randomly selected fetuses had TO. We harvested fetuses at GD23, GD28, or GD30. 
      We calculated lung-to-body weight ratios, processed lungs for miR-200b in situ 
      hybridization and real-time quantitative polymerase chain reaction, and evaluated 
      effects on downstream targets TGF-beta2 or ZEB2. RESULTS: We obtained 16 DH fetuses 
      (n = 7 GD28 and n = 9 GD30), 13 TO fetuses (GD30), and 38 control fetuses (n = 15 
      GD23, n = 11 GD28, and n = 12 GD30). Diaphragmatic hernia lungs were hypoplastic, 
      and TO resulted in control lung-to-body weight ratio levels. Term miR-200b-3p 
      levels were significantly upregulated in the hypoplastic compared with control 
      ipsilateral lung (1.906 +/- 0.90 vs 0.7429 +/- 0.44) (P < .01). Fetal TO ipsilateral 
      lungs displayed a variable miR-200b response on in situ hybridization and 
      polymerase chain reaction, with levels similar to control and congenital DH 
      lungs. The TGF-beta2 was unchanged in hypoplastic and TO lungs, and ZEB2 tended to 
      be reduced in TO compared with DH lungs (1.79 [0.4-2.9] vs 0.73 [0.5-1.4]). 
      CONCLUSIONS: Hypoplastic fetal rabbit lungs display upregulation of miR-200b 
      expression although downstream targets are not different from controls. Following 
      TO, fetal rabbit lungs display a variable miR-200b response.
CI  - (c) 2018 John Wiley & Sons, Ltd.
FAU - Eastwood, Mary Patrice
AU  - Eastwood MP
AUID- ORCID: 0000-0002-6437-8972
AD  - Cluster Organ Systems, Department of Development and Regeneration, and Center for 
      Surgical Technologies, Group Biomedical Sciences, KU Leuven, Leuven, Belgium.
FAU - Deprest, Jan
AU  - Deprest J
AD  - Cluster Organ Systems, Department of Development and Regeneration, and Center for 
      Surgical Technologies, Group Biomedical Sciences, KU Leuven, Leuven, Belgium.
AD  - Fetal Medicine Unit, Clinical Department of Obstetrics and Gynaecology, 
      University Hospitals Leuven, Leuven, Belgium.
AD  - Research Department of Maternal Fetal Medicine, Institute for Women's Health 
      (IWH), University College London, London, UK.
FAU - Russo, Francesca Maria
AU  - Russo FM
AUID- ORCID: 0000-0002-5029-7899
AD  - Cluster Organ Systems, Department of Development and Regeneration, and Center for 
      Surgical Technologies, Group Biomedical Sciences, KU Leuven, Leuven, Belgium.
AD  - Fetal Medicine Unit, Clinical Department of Obstetrics and Gynaecology, 
      University Hospitals Leuven, Leuven, Belgium.
FAU - Wang, Hongmei
AU  - Wang H
AD  - Cluster Organ Systems, Department of Development and Regeneration, and Center for 
      Surgical Technologies, Group Biomedical Sciences, KU Leuven, Leuven, Belgium.
FAU - Mulhall, Drew
AU  - Mulhall D
AD  - Department of Surgery, Pediatrics & Child Health, University of Manitoba, 
      Winnipeg, MB, Canada.
AD  - Department of Physiology, University of Manitoba, Winnipeg, MB, Canada.
AD  - Biology of Breathing, Children's Hospital Research Institute of Manitoba, 
      Winnipeg, MB, Canada.
FAU - Iwasiow, Barbara
AU  - Iwasiow B
AD  - Department of Surgery, Pediatrics & Child Health, University of Manitoba, 
      Winnipeg, MB, Canada.
AD  - Department of Physiology, University of Manitoba, Winnipeg, MB, Canada.
AD  - Biology of Breathing, Children's Hospital Research Institute of Manitoba, 
      Winnipeg, MB, Canada.
FAU - Mahood, Thomas H
AU  - Mahood TH
AD  - Department of Surgery, Pediatrics & Child Health, University of Manitoba, 
      Winnipeg, MB, Canada.
AD  - Department of Physiology, University of Manitoba, Winnipeg, MB, Canada.
AD  - Biology of Breathing, Children's Hospital Research Institute of Manitoba, 
      Winnipeg, MB, Canada.
FAU - Keijzer, Richard
AU  - Keijzer R
AUID- ORCID: 0000-0002-0108-3157
AD  - Department of Surgery, Pediatrics & Child Health, University of Manitoba, 
      Winnipeg, MB, Canada.
AD  - Department of Physiology, University of Manitoba, Winnipeg, MB, Canada.
AD  - Biology of Breathing, Children's Hospital Research Institute of Manitoba, 
      Winnipeg, MB, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180717
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Female
MH  - Fetal Weight
MH  - Gestational Age
MH  - Hernias, Diaphragmatic, Congenital/etiology/*metabolism
MH  - In Situ Hybridization/veterinary
MH  - Lung/*chemistry/*embryology/metabolism
MH  - MicroRNAs/*analysis/genetics
MH  - Organ Size
MH  - Pregnancy
MH  - Rabbits
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Trachea/surgery
MH  - Up-Regulation
EDAT- 2018/06/23 06:00
MHDA- 2019/04/09 06:00
CRDT- 2018/06/23 06:00
PHST- 2018/02/13 00:00 [received]
PHST- 2018/06/13 00:00 [revised]
PHST- 2018/06/14 00:00 [accepted]
PHST- 2018/06/23 06:00 [pubmed]
PHST- 2019/04/09 06:00 [medline]
PHST- 2018/06/23 06:00 [entrez]
AID - 10.1002/pd.5318 [doi]
PST - ppublish
SO  - Prenat Diagn. 2018 Aug;38(9):645-653. doi: 10.1002/pd.5318. Epub 2018 Jul 17.