PMID- 29932639
OWN - NLM
STAT- MEDLINE
DCOM- 20190326
LR  - 20191210
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 90
IP  - 14
DP  - 2018 Jul 17
TI  - Method for Electrochemical Detection of Brain Derived Neurotrophic Factor (BDNF) 
      in Plasma.
PG  - 8561-8566
LID - 10.1021/acs.analchem.8b01642 [doi]
AB  - Currently, a blood test for the diagnosis of endometriosis, a common 
      estrogen-dependent gynecological disease, does not exist. Recent studies suggest 
      that circulating concentrations of brain-derived neurotrophic factor (BDNF) have 
      potential for the diagnosis of endometriosis. However, at present, BDNF can only 
      be measured by ELISA, which requires a clinic visit, a routine blood sample, and 
      laboratory testing. Therefore, we developed a point-of-care device (EndoChip) for 
      use with small blood volumes that can be collected through a finger prick. 
      Specifically, the presented device is a polymer-based chip with a nanoporous, 
      wrinkled gold film acting as the electrode/sensing layer, allowing for the 
      electrochemical detection of BDNF in plasma. Increasing concentrations of BDNF 
      (0.1-2.0 ng/mL) induced significant differences in redox current. The biosensor 
      produces a signal readout in a matter of seconds, and is ideal for realizing 
      multiplexing. Blood samples were collected from women ( n = 20) with chronic 
      pelvic pain undergoing a diagnostic laparoscopy. Plasma BDNF concentrations 
      measured by commercial ELISA were positively correlated ( r(2) = 0.8033; p < 
      0.001) with results from the EndoChip. Our results demonstrate a quick and 
      reliable method for point-of-care quantification of circulating concentrations of 
      BDNF and a promising diagnostic tool for endometriosis.
FAU - Bockaj, M
AU  - Bockaj M
FAU - Fung, B
AU  - Fung B
FAU - Tsoulis, M
AU  - Tsoulis M
FAU - Foster, W G
AU  - Foster WG
AUID- ORCID: 0000-0001-5762-7371
FAU - Soleymani, L
AU  - Soleymani L
AUID- ORCID: 0000-0003-4915-2999
LA  - eng
GR  - MOP142230/CIHR/Canada
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180706
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Polymers)
RN  - 7171WSG8A2 (BDNF protein, human)
RN  - 7440-57-5 (Gold)
SB  - IM
CIN - Ann Transl Med. 2018 Dec;6(Suppl 2):S106. PMID: 30740427
MH  - Adolescent
MH  - Adult
MH  - Biosensing Techniques/*instrumentation
MH  - Brain-Derived Neurotrophic Factor/*blood
MH  - Electrochemical Techniques/instrumentation
MH  - Electrodes
MH  - Endometriosis/*blood/diagnosis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Equipment Design
MH  - Female
MH  - Gold/chemistry
MH  - Humans
MH  - Lab-On-A-Chip Devices
MH  - Limit of Detection
MH  - Middle Aged
MH  - *Point-of-Care Systems
MH  - Polymers/chemistry
MH  - Young Adult
EDAT- 2018/06/23 06:00
MHDA- 2019/03/27 06:00
CRDT- 2018/06/23 06:00
PHST- 2018/06/23 06:00 [pubmed]
PHST- 2019/03/27 06:00 [medline]
PHST- 2018/06/23 06:00 [entrez]
AID - 10.1021/acs.analchem.8b01642 [doi]
PST - ppublish
SO  - Anal Chem. 2018 Jul 17;90(14):8561-8566. doi: 10.1021/acs.analchem.8b01642. Epub 
      2018 Jul 6.