PMID- 29934970
OWN - NLM
STAT- MEDLINE
DCOM- 20191125
LR  - 20191125
IS  - 1439-7633 (Electronic)
IS  - 1439-4227 (Linking)
VI  - 20
IP  - 4
DP  - 2019 Feb 15
TI  - A Bioluminescent Ca(2+) Indicator Based on a Topological Variant of GCaMP6s.
PG  - 516-520
LID - 10.1002/cbic.201800255 [doi]
AB  - Fluorescent genetically encoded calcium ion indicators (GECIs) enable Ca(2+) 
      dynamics to be monitored in a diverse array of cell types and tissues. One 
      drawback of green fluorescent GECIs, such as the widely used GCaMP6, is that the 
      blue wavelengths of light used to excite the GECI also activate optogenetic 
      actuators such as channelrhodopsins. Accordingly, it is particularly challenging 
      simultaneously to use both optogenetic actuators and GECIs to both control and 
      image cell signaling. Bioluminescence is an alternative imaging modality that 
      circumvents this problem by avoiding the need for illumination for fluorescence 
      excitation. Here, we report the development of a bioluminescent GECI, designated 
      LUCI-GECO1, based on efficient bioluminescent resonance energy transfer (BRET) 
      between the NanoLuc luciferase and a topological variant of GCaMP6s. LUCI-GECO1 
      is a sensitive ratiometric GECI that retains the highly optimized properties of 
      GCaMP6s, as we demonstrate by imaging of chemically and optogenetically induced 
      Ca(2+) concentration changes in cultured cells and neurons.
CI  - (c) 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Qian, Yong
AU  - Qian Y
AD  - University of Alberta, Department of Chemistry, 11227 Saskatchewan Drive, 
      Edmonton, Alberta, T6G 2G2, Canada.
FAU - Rancic, Vladimir
AU  - Rancic V
AD  - University of Alberta, Department of Physiology, 7-55 Medical Sciences Building, 
      Edmonton, Alberta, T6G 2H7, Canada.
FAU - Wu, Jiahui
AU  - Wu J
AD  - University of Alberta, Department of Chemistry, 11227 Saskatchewan Drive, 
      Edmonton, Alberta, T6G 2G2, Canada.
FAU - Ballanyi, Klaus
AU  - Ballanyi K
AD  - University of Alberta, Department of Physiology, 7-55 Medical Sciences Building, 
      Edmonton, Alberta, T6G 2H7, Canada.
FAU - Campbell, Robert E
AU  - Campbell RE
AUID- ORCID: 0000-0003-0604-092X
AD  - University of Alberta, Department of Chemistry, 11227 Saskatchewan Drive, 
      Edmonton, Alberta, T6G 2G2, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180731
PL  - Germany
TA  - Chembiochem
JT  - Chembiochem : a European journal of chemical biology
JID - 100937360
RN  - 0 (Calmodulin)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 1.13.12.- (Luciferases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/chemistry/*metabolism
MH  - Calmodulin/genetics/metabolism
MH  - *Fluorescence Resonance Energy Transfer
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Luciferases/genetics/metabolism
MH  - Neurons/metabolism
MH  - Optical Imaging
MH  - Rats
OTO - NOTNLM
OT  - BRET
OT  - bioluminescence
OT  - calcium
OT  - cell imaging
OT  - indicators
EDAT- 2018/06/24 06:00
MHDA- 2019/11/26 06:00
CRDT- 2018/06/24 06:00
PHST- 2018/05/12 00:00 [received]
PHST- 2018/06/24 06:00 [pubmed]
PHST- 2019/11/26 06:00 [medline]
PHST- 2018/06/24 06:00 [entrez]
AID - 10.1002/cbic.201800255 [doi]
PST - ppublish
SO  - Chembiochem. 2019 Feb 15;20(4):516-520. doi: 10.1002/cbic.201800255. Epub 2018 
      Jul 31.