PMID- 29935099
OWN - NLM
STAT- MEDLINE
DCOM- 20190910
LR  - 20210109
IS  - 1098-2396 (Electronic)
IS  - 0887-4476 (Print)
IS  - 0887-4476 (Linking)
VI  - 72
IP  - 12
DP  - 2018 Dec
TI  - Presynaptic GCaMP expression decreases vesicle release probability at the calyx 
      of Held.
PG  - e22040
LID - 10.1002/syn.22040 [doi]
LID - e22040
AB  - Synaptic vesicle (SV) exocytosis is intimately dependent on free local Ca(2+) 
      near active zones. Genetically encoded calcium indicators (GECIs) have become an 
      indispensable tool to monitor calcium dynamics during physiological responses, 
      and they are widely used as a proxy to monitor activity in neuronal ensembles and 
      at synaptic terminals. However, GECIs' ability to bind Ca(2+) at physiologically 
      relevant concentration makes them strong candidates to affect calcium homeostasis 
      and alter synaptic transmission by exogenously increasing Ca(2+) buffering. In 
      the present study, we show that genetically expressed GCaMP6m modulates SV 
      release probability at the mouse calyx of Held synapse. GCaMP6m expression for 
      approximately three weeks decreased initial SV release for both low-frequency 
      stimulation and high-frequency stimulation trains, and slowed presynaptic 
      short-term depression. However, GCaMP6m does not affect quantal events during 
      spontaneous activity at this synapse. This study emphasizes the careful use of 
      GECIs as monitors of neuronal activity and inspects the role of these transgenic 
      indicators which may alter calcium-dependent physiological responses.
CI  - (c) 2018 The Authors. Synapse Published by Wiley Periodicals, Inc.
FAU - Singh, Mahendra
AU  - Singh M
AD  - Department of Physiology and Cell Biology, University of Nevada, Reno, Nevada, 
      89557.
FAU - Lujan, Brendan
AU  - Lujan B
AD  - Department of Physiology and Cell Biology, University of Nevada, Reno, Nevada, 
      89557.
AD  - Currently at Vollum Institute, Oregon Health and Science University, Portland, 
      Oregon.
FAU - Renden, Robert
AU  - Renden R
AUID- ORCID: 0000-0001-7905-4789
AD  - Department of Physiology and Cell Biology, University of Nevada, Reno, Nevada, 
      89557.
LA  - eng
GR  - P20 GM103554/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20180717
PL  - United States
TA  - Synapse
JT  - Synapse (New York, N.Y.)
JID - 8806914
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Luminescent Proteins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/metabolism
MH  - Calcium-Binding Proteins/genetics/*metabolism
MH  - *Exocytosis
MH  - Female
MH  - Genes, Reporter
MH  - Luminescent Proteins/genetics/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Presynaptic Terminals/metabolism
MH  - Synaptic Vesicles/*metabolism
PMC - PMC6186185
MID - NIHMS987362
OTO - NOTNLM
OT  - animals
OT  - calcium
OT  - exocytosis
OT  - mice
OT  - presynaptic terminals
OT  - synaptic transmission
OT  - synaptic vesicles
EDAT- 2018/06/24 06:00
MHDA- 2019/09/11 06:00
PMCR- 2018/11/07
CRDT- 2018/06/24 06:00
PHST- 2018/05/03 00:00 [received]
PHST- 2018/06/04 00:00 [revised]
PHST- 2018/06/05 00:00 [accepted]
PHST- 2018/06/24 06:00 [pubmed]
PHST- 2019/09/11 06:00 [medline]
PHST- 2018/06/24 06:00 [entrez]
PHST- 2018/11/07 00:00 [pmc-release]
AID - SYN22040 [pii]
AID - 10.1002/syn.22040 [doi]
PST - ppublish
SO  - Synapse. 2018 Dec;72(12):e22040. doi: 10.1002/syn.22040. Epub 2018 Jul 17.