PMID- 29935189
OWN - NLM
STAT- MEDLINE
DCOM- 20190411
LR  - 20190411
IS  - 1879-3185 (Electronic)
IS  - 0300-483X (Linking)
VI  - 408
DP  - 2018 Sep 1
TI  - Ultrastructural and biochemical features of cerebral microvessels of adult rat 
      subjected to a low dose of silver nanoparticles.
PG  - 31-38
LID - S0300-483X(18)30121-5 [pii]
LID - 10.1016/j.tox.2018.06.009 [doi]
AB  - The widespread use of silver nanoparticles (AgNPs) in medicine and in multiple 
      commercial products has motivated researchers to investigate their potentially 
      hazardous effects in organisms. Since AgNPs may easily enter the brain through 
      the blood-brain barrier (BBB), characterization of their interactions with 
      cellular components of the neurovascular unit (NVU) is of particular importance. 
      Therefore, in an animal model of prolonged low-dose exposure, we investigate the 
      extent and mechanisms of influence of AgNPs on cerebral microvessels. Adult rats 
      were treated orally with small (10 nm) AgNPs in a dose of 0.2 mg/kg b.w. over a 
      2-week period. A silver citrate-exposed group was established as a positive 
      control of ionic silver effects. Alterations in the expression of tight junction 
      proteins claudin-5, ZO-1, and occludin, were observed. These effects are 
      accompanied by ultrastructural features indicating enhanced permeability of 
      microvessels such as focal edema of perivascular astrocytic processes and 
      surrounding neuropil. We did not identify changes in the expression of PDGFbetaR 
      which is a marker of pericytes. Ultrastructural alterations in these cells were 
      not identified. The results show that altered integrity of cerebral vessels under 
      a low-dose of AgNP-exposure may be the consequence of dysfunction of endothelial 
      cells caused by disruption of tight junction proteins.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Dabrowska-Bouta, Beata
AU  - Dabrowska-Bouta B
AD  - Laboratory of Pathoneurochemistry, Department of Neurochemistry, Mossakowski 
      Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego str., 02-106 
      Warsaw, Poland.
FAU - Sulkowski, Grzegorz
AU  - Sulkowski G
AD  - Laboratory of Pathoneurochemistry, Department of Neurochemistry, Mossakowski 
      Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego str., 02-106 
      Warsaw, Poland.
FAU - Frontczak-Baniewicz, Malgorzata
AU  - Frontczak-Baniewicz M
AD  - Electron Microscopy Platform, Mossakowski Medical Research Centre, Polish Academy 
      of Sciences, 5 Pawinskiego str., 02-106 Warsaw, Poland.
FAU - Skalska, Joanna
AU  - Skalska J
AD  - Laboratory of Pathoneurochemistry, Department of Neurochemistry, Mossakowski 
      Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego str., 02-106 
      Warsaw, Poland.
FAU - Salek, Mikolaj
AU  - Salek M
AD  - Laboratory of Pathoneurochemistry, Department of Neurochemistry, Mossakowski 
      Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego str., 02-106 
      Warsaw, Poland.
FAU - Orzelska-Gorka, Jolanta
AU  - Orzelska-Gorka J
AD  - Department of Pharmacology and Pharmacodynamics, Medical University of Lublin, 4a 
      Chodzki str., 20-093 Lublin, Poland.
FAU - Struzynska, Lidia
AU  - Struzynska L
AD  - Laboratory of Pathoneurochemistry, Department of Neurochemistry, Mossakowski 
      Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego str., 02-106 
      Warsaw, Poland. Electronic address: lidkas@imdik.pan.pl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180620
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Citrates)
RN  - 0 (Claudin-5)
RN  - 0 (Cldn5 protein, rat)
RN  - 0 (Occludin)
RN  - 0 (Ocln protein, rat)
RN  - 0 (Silver Compounds)
RN  - 0 (Tjp1 protein, rat)
RN  - 0 (Zonula Occludens-1 Protein)
RN  - EC 2.7.10.1 (Receptor, Platelet-Derived Growth Factor beta)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Brain/*blood supply
MH  - Capillaries/*drug effects/metabolism/ultrastructure
MH  - Capillary Permeability/drug effects
MH  - Citrates/administration & dosage/*toxicity
MH  - Claudin-5/genetics/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Male
MH  - Metal Nanoparticles/*toxicity
MH  - Occludin/genetics/metabolism
MH  - Particle Size
MH  - Rats, Wistar
MH  - Receptor, Platelet-Derived Growth Factor beta/genetics/metabolism
MH  - Silver Compounds/administration & dosage/*toxicity
MH  - Tight Junctions/drug effects/metabolism/ultrastructure
MH  - Time Factors
MH  - Zonula Occludens-1 Protein/genetics/metabolism
OTO - NOTNLM
OT  - Blood-brain barrier
OT  - Nanosilver
OT  - Nanotoxicity
OT  - Occludin
OT  - Tight junction proteins
OT  - ZO-1
OT  - claudin-5
EDAT- 2018/06/24 06:00
MHDA- 2019/04/12 06:00
CRDT- 2018/06/24 06:00
PHST- 2018/01/08 00:00 [received]
PHST- 2018/06/15 00:00 [revised]
PHST- 2018/06/19 00:00 [accepted]
PHST- 2018/06/24 06:00 [pubmed]
PHST- 2019/04/12 06:00 [medline]
PHST- 2018/06/24 06:00 [entrez]
AID - S0300-483X(18)30121-5 [pii]
AID - 10.1016/j.tox.2018.06.009 [doi]
PST - ppublish
SO  - Toxicology. 2018 Sep 1;408:31-38. doi: 10.1016/j.tox.2018.06.009. Epub 2018 Jun 
      20.