PMID- 29936767
OWN - NLM
STAT- MEDLINE
DCOM- 20180706
LR  - 20190221
IS  - 0253-3766 (Print)
IS  - 0253-3766 (Linking)
VI  - 40
IP  - 6
DP  - 2018 Jun 23
TI  - [The application of conventional smears and liquid-based cytologic slides of 
      lymphatic metastasis specimens of lung adenocarcinoma acquired by fine needle 
      aspiration to detect the expression of anaplastic lymphoma kinase protein].
PG  - 422-427
LID - 10.3760/cma.j.issn.0253-3766.2018.06.005 [doi]
AB  - Objective: To explore the feasibility of conventional smears and liquid-based 
      cytologic slides of lymphatic metastasis specimens of lung adenocarcinoma 
      acquired by fine needle aspiration cytology (FNAC) to detect the expression of 
      anaplastic lymphoma kinase (ALK/D5F3) by immunocytochemistry (ICC) analysis. 
      Methods: The lymphatic metastasis specimens of 147 lung adenocarcinoma, including 
      100 liquid-based cytologic slides and 47 conventional smears, were collected in 
      this study. ALK fusion protein was detected by Roche Ventana ICC technology, 
      which was compared with the ALK fusion gene assessed by fluorescence in situ 
      hybridization (FISH) or reverse transcriptase-polymerase chain reaction (RT-PCR). 
      Results: The positive rate of ALK (D5F3) fusion protein in advanced lung 
      adenocarcinoma acquired by FNAC was 11.6% (17/147), and 10.6% (5/47) and 12.0% 
      (12/100) were reached in conventional smears and liquid-based cytologic slides, 
      respectively.Among 147 cases, 57 cases including 17 positive cases and 40 
      negative cases were verified by RT-PCR and FISH. The whole coincidence rate 
      reached 96.5% (55/57). The sensitivity and specificity of ALK (D5F3) fusion 
      protein detected in lung adenocarcinoma acquired by FNAC were 94.1% (16/17) and 
      97.5% (39/40), respectively. The sensitivity and specificity were both 100% (5/5 
      of sensitivity and 10/10 of specificity) in conventional smears, while 91.7% 
      (11/12) and 96.7% (29/30) in liquid-based cytologic slides. Conclusion: 
      Conventional smears and liquid-based cytologic slides of FNAC samples can be used 
      to perform ICC analysis of ALK (D5F3) expression in advanced lung adenocarcinoma, 
      especially for patients who have no opportunity for surgery or whose resected 
      samples are difficult to form cell block.
FAU - Jia, J
AU  - Jia J
AD  - Department of Pathology, National Cancer Center/Cancer Hospital, Chinese Academy 
      of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
FAU - Zhao, L L
AU  - Zhao LL
AD  - Department of Pathology, National Cancer Center/Cancer Hospital, Chinese Academy 
      of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
FAU - Guo, H Q
AU  - Guo HQ
AD  - Department of Pathology, National Cancer Center/Cancer Hospital, Chinese Academy 
      of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
FAU - Zhao, H
AU  - Zhao H
AD  - Department of Pathology, National Cancer Center/Cancer Hospital, Chinese Academy 
      of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
FAU - Zhang, Z H
AU  - Zhang ZH
AD  - Department of Pathology, National Cancer Center/Cancer Hospital, Chinese Academy 
      of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
FAU - Guo, L
AU  - Guo L
AD  - Department of Pathology, National Cancer Center/Cancer Hospital, Chinese Academy 
      of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
FAU - Ying, J M
AU  - Ying JM
AD  - Department of Pathology, National Cancer Center/Cancer Hospital, Chinese Academy 
      of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Zhong Liu Za Zhi
JT  - Zhonghua zhong liu za zhi [Chinese journal of oncology]
JID - 7910681
RN  - EC 2.7.10.1 (ALK protein, human)
RN  - EC 2.7.10.1 (Anaplastic Lymphoma Kinase)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Adenocarcinoma/*chemistry/pathology
MH  - Adenocarcinoma of Lung
MH  - Anaplastic Lymphoma Kinase
MH  - Biopsy, Fine-Needle
MH  - Cytodiagnosis
MH  - Gene Rearrangement
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Liquid Biopsy
MH  - Lung Neoplasms/*chemistry/pathology
MH  - Lymphatic Metastasis
MH  - Receptor Protein-Tyrosine Kinases/*analysis
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - Anaplastic lymphoma kinase, ALK
OT  - Conventional smears
OT  - Fine needle aspiration
OT  - Liquid based cytologic slides
OT  - Lung neoplasms
EDAT- 2018/06/26 06:00
MHDA- 2018/07/07 06:00
CRDT- 2018/06/26 06:00
PHST- 2018/06/26 06:00 [entrez]
PHST- 2018/06/26 06:00 [pubmed]
PHST- 2018/07/07 06:00 [medline]
AID - 10.3760/cma.j.issn.0253-3766.2018.06.005 [doi]
PST - ppublish
SO  - Zhonghua Zhong Liu Za Zhi. 2018 Jun 23;40(6):422-427. doi: 
      10.3760/cma.j.issn.0253-3766.2018.06.005.