PMID- 29937123
OWN - NLM
STAT- MEDLINE
DCOM- 20180813
LR  - 20181202
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1565
DP  - 2018 Aug 31
TI  - Thorough study of persistent organic pollutants and halogenated natural products 
      in sperm whale blubber through preparative sample cleanup followed by 
      fractionation with countercurrent chromatography.
PG  - 114-123
LID - S0021-9673(18)30793-3 [pii]
LID - 10.1016/j.chroma.2018.06.037 [doi]
AB  - Marine mammals are top predators of the marine food chain and thus known to 
      bioaccumulate high concentrations of polyhalogenated compounds. Yet, details of 
      the organohalogen pattern are largely unknown. For this reason, we isolated the 
      polyhalogenated compounds from 750 g blubber of a sperm whale (Physeter catodon), 
      which deceased at the German North Sea coast in January 2016. The sample matrix 
      was decomposed by sulfuric acid treatment and the polyhalogenated compounds were 
      then fractionated by countercurrent chromatography (CCC). Seventy-three CCC 
      fractions were taken and analyzed by gas chromatography with electron capture 
      negative ion mass spectrometry (GC/ECNI-MS). The bulk of the polyhalogenated 
      compounds in the sample originated from classic persistent organic pollutants 
      (POPs). Altogether 90 polychlorinated biphenyl (PCB) congeners were detected in 
      the sample including all possible octa- to decachloro congeners except one. The 
      sample also featured 105 toxaphene congeners including 30 chlorobornenes 
      (contribution  approximately 14% of the total toxaphene content) which were only detected after 
      the CCC fractionation. In addition, several chlordane and mirex related compounds 
      were detected which were never or very scarcely described before in biota. 
      Classic POPs (PCBs, DDT, toxaphene, chlordane, mirex) were predominant, while new 
      emerging contaminants were scarcely detected. The sample featured several 
      halogenated natural products (HNPs) some of which were less stable and destroyed 
      during the sample cleanup involving treatment with sulfuric acid. Sample 
      fractionation by means of CCC was crucial for the detection and assignment of 
      many of the uncommon polyhalogenated compounds.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Vetter, Walter
AU  - Vetter W
AD  - University of Hohenheim, Institute of Food Chemistry (170b), Garbenstrasse 28, 
      D-70599 Stuttgart, Germany. Electronic address: walter.vetter@uni-hohenheim.de.
FAU - Lok, Bianca
AU  - Lok B
AD  - University of Hohenheim, Institute of Food Chemistry (170b), Garbenstrasse 28, 
      D-70599 Stuttgart, Germany.
FAU - Schlienz, Annika
AU  - Schlienz A
AD  - University of Hohenheim, Institute of Food Chemistry (170b), Garbenstrasse 28, 
      D-70599 Stuttgart, Germany.
FAU - Muller, Marco
AU  - Muller M
AD  - University of Hohenheim, Institute of Food Chemistry (170b), Garbenstrasse 28, 
      D-70599 Stuttgart, Germany.
LA  - eng
PT  - Journal Article
DEP - 20180619
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Biological Products)
RN  - 0 (Organic Chemicals)
RN  - 0 (Water Pollutants, Chemical)
RN  - 8001-35-2 (Toxaphene)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
SB  - IM
MH  - Adipose Tissue/chemistry
MH  - Animal Structures/*metabolism
MH  - Animals
MH  - Biological Products/*analysis
MH  - Chemical Fractionation
MH  - Countercurrent Distribution/*methods
MH  - *Environmental Monitoring
MH  - Gas Chromatography-Mass Spectrometry
MH  - *Halogenation
MH  - Human Activities
MH  - Humans
MH  - Male
MH  - Organic Chemicals/*analysis
MH  - Polychlorinated Biphenyls/analysis
MH  - Sperm Whale/*anatomy & histology
MH  - Toxaphene/analysis
MH  - Water Pollutants, Chemical/*analysis
OTO - NOTNLM
OT  - Countercurrent chromatography
OT  - GC/MS
OT  - Halogenated natural products
OT  - Marine mammals
OT  - Persistent organic pollutants
EDAT- 2018/06/26 06:00
MHDA- 2018/08/14 06:00
CRDT- 2018/06/26 06:00
PHST- 2018/02/28 00:00 [received]
PHST- 2018/06/11 00:00 [revised]
PHST- 2018/06/16 00:00 [accepted]
PHST- 2018/06/26 06:00 [pubmed]
PHST- 2018/08/14 06:00 [medline]
PHST- 2018/06/26 06:00 [entrez]
AID - S0021-9673(18)30793-3 [pii]
AID - 10.1016/j.chroma.2018.06.037 [doi]
PST - ppublish
SO  - J Chromatogr A. 2018 Aug 31;1565:114-123. doi: 10.1016/j.chroma.2018.06.037. Epub 
      2018 Jun 19.