PMID- 29938372
OWN - NLM
STAT- MEDLINE
DCOM- 20180905
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 410
IP  - 23
DP  - 2018 Sep
TI  - Antifouling and ultrasensitive biosensing interface based on self-assembled 
      peptide and aptamer on macroporous gold for electrochemical detection of 
      immunoglobulin E in serum.
PG  - 5871-5878
LID - 10.1007/s00216-018-1201-9 [doi]
AB  - Accurate detection of protein biomarkers in complex media remains a challenge due 
      to severe nonspecific adsorption and biofouling, and sensing interfaces that 
      combine the high sensitivity and antifouling ability are highly desirable. 
      Herein, an antifouling sensing interface capable of sensitively assaying 
      immunoglobulin E (IgE) in biological samples was constructed. The sensing 
      interface was fabricated through the self-assembly of a zwitterionic peptide and 
      the IgE aptamer onto a macroporous Au substrate, which was electrochemically 
      fabricated with the aid of multilayer polystyrene nanospheres self-assembled on 
      glassy carbon electrode. Due to the huge surface area arising from porous 
      morphology and high specificity of aptamer, the developed electrochemical 
      biosensor exhibits ultrahigh sensitivity and selectivity towards IgE, with the 
      linear range of 0.1-10 pg mL(-1), and a very low limit of detection down to 
      42 fg mL(-1). Interestingly, owing to the presence of the zwitterionic peptide, 
      the biosensing interface can satisfyingly reduce the nonspecific adsorption and 
      fouling effect. Consequently, the biosensor was successfully applied to detect 
      IgE in complex biological samples, indicating great promise of this peptide-based 
      sensing interface for antifouling assays. Graphical abstract ᅟ.
FAU - Wang, Yu
AU  - Wang Y
AD  - Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, Qingdao 
      University of Science and Technology, Qingdao, 266042, Shandong, China.
AD  - Shandong Key Laboratory of Biochemical Analysis, Qingdao University of Science 
      and Technology, Qingdao, 266042, Shandong, China.
AD  - Key Laboratory of Analytical Chemistry for Life Science in Universities of 
      Shandong, College of Chemistry and Molecular Engineering, Qingdao University of 
      Science and Technology, Qingdao, 266042, Shandong, China.
FAU - Cui, Min
AU  - Cui M
AD  - College of Chemistry and Chemical Engineering, University of Jinan, Jinan, 
      250022, Shandong, China.
FAU - Jiao, Mingxia
AU  - Jiao M
AUID- ORCID: 0000-0002-1841-3052
AD  - Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, Qingdao 
      University of Science and Technology, Qingdao, 266042, Shandong, China. 
      mingxjiao@163.com.
AD  - Shandong Key Laboratory of Biochemical Analysis, Qingdao University of Science 
      and Technology, Qingdao, 266042, Shandong, China. mingxjiao@163.com.
AD  - Key Laboratory of Analytical Chemistry for Life Science in Universities of 
      Shandong, College of Chemistry and Molecular Engineering, Qingdao University of 
      Science and Technology, Qingdao, 266042, Shandong, China. mingxjiao@163.com.
FAU - Luo, Xiliang
AU  - Luo X
AD  - Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, Qingdao 
      University of Science and Technology, Qingdao, 266042, Shandong, China.
AD  - Shandong Key Laboratory of Biochemical Analysis, Qingdao University of Science 
      and Technology, Qingdao, 266042, Shandong, China.
AD  - Key Laboratory of Analytical Chemistry for Life Science in Universities of 
      Shandong, College of Chemistry and Molecular Engineering, Qingdao University of 
      Science and Technology, Qingdao, 266042, Shandong, China.
LA  - eng
GR  - 21675093/National Natural Science Foundation of China/
GR  - ts20110829/Taishan Scholar Program of Shandong Province of China/
GR  - ZR2017BB009/Natural Science Foundation of Shandong Province of China/
PT  - Evaluation Study
PT  - Journal Article
DEP - 20180625
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Peptides)
RN  - 0 (Polystyrenes)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - Biofouling/prevention & control
MH  - Biosensing Techniques/instrumentation/*methods
MH  - Electrochemical Techniques/instrumentation/methods
MH  - Electrodes
MH  - Gold/*chemistry
MH  - Humans
MH  - Immunoglobulin E/*blood
MH  - Limit of Detection
MH  - Models, Molecular
MH  - Nanospheres/chemistry
MH  - Peptides/*chemistry
MH  - Polystyrenes/chemistry
OTO - NOTNLM
OT  - Antifouling
OT  - Aptamer
OT  - Electrochemical biosensor
OT  - Immunoglobulin E
OT  - Peptide
EDAT- 2018/06/26 06:00
MHDA- 2018/09/06 06:00
CRDT- 2018/06/26 06:00
PHST- 2018/05/02 00:00 [received]
PHST- 2018/06/14 00:00 [accepted]
PHST- 2018/06/11 00:00 [revised]
PHST- 2018/06/26 06:00 [pubmed]
PHST- 2018/09/06 06:00 [medline]
PHST- 2018/06/26 06:00 [entrez]
AID - 10.1007/s00216-018-1201-9 [pii]
AID - 10.1007/s00216-018-1201-9 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2018 Sep;410(23):5871-5878. doi: 10.1007/s00216-018-1201-9. 
      Epub 2018 Jun 25.