PMID- 29938841
OWN - NLM
STAT- MEDLINE
DCOM- 20181218
LR  - 20181218
IS  - 1439-0507 (Electronic)
IS  - 0933-7407 (Linking)
VI  - 61
IP  - 11
DP  - 2018 Nov
TI  - Polyclonal antibodies to Paracoccidioides brasiliensis are able to recognise 
      antigens from different strains from Paracoccidioides species complex, including 
      Paracoccidioides lutzii LDR2.
PG  - 826-832
LID - 10.1111/myc.12819 [doi]
AB  - Since the new species Paracoccidioides lutzii emerged in 2009, much has been 
      researched on strains previously considered atypical. Still, there is no 
      consensus about recognition of antigens from P. lutzii by antibodies directed to 
      other Paracoccidioides species, which can have great impact on 
      Paracoccidioidomycosis (PCM) diagnosis. Current research investigated soluble 
      protein/carbohydrate epitopes from P. lutzii LDR2, Paracoccidioides restrepiensis 
      B339 and Paracoccidioides americana LDR3 recognised by IgG directed to 
      Paracoccidioides brasiliensis. Cell free antigens (CFA) were analysed by: 
      (a)silver and periodic acid-Schiff staining of SDS-PAGE; (b)immunoblot (IB) with 
      rabbit IgG anti-P. brasiliensis Pb18; (c)IB and ELISA with a pool of PCM 
      patients' sera before and after treatment with sodium metaperiodate (SMP) to 
      oxidise carbohydrate epitopes. Both rabbit and human immune sera recognised 
      several antigens of P. lutzii LDR2, P. restrepiensis B339 and P. americana LDR3. 
      P. lutzii's gp43 was not observed in IB or silver/PAS staining. SMP treatment 
      affected reactions with all 3 CFAs, but more intensely with antigens from 
      P. lutzii LDR2. In conclusion, antibodies directed to P. brasiliensis recognised 
      antigens from P. lutzii LDR2. The use of any of the recognised antigens in a 
      broad spectrum diagnostic model for Paracoccidioides species complex needs to be 
      further investigated.
CI  - (c) 2018 Blackwell Verlag GmbH.
FAU - Lenhard-Vidal, Adriane
AU  - Lenhard-Vidal A
AUID- ORCID: 0000-0002-1597-6853
AD  - State University of Londrina, Londrina, Brazil.
FAU - Assolini, Joao P
AU  - Assolini JP
AUID- ORCID: 0000-0003-4190-8835
AD  - State University of Londrina, Londrina, Brazil.
FAU - Chiyoda, Franciele A S
AU  - Chiyoda FAS
AD  - State University of Londrina, Londrina, Brazil.
FAU - Ono, Mario A
AU  - Ono MA
AUID- ORCID: 0000-0002-8691-5118
AD  - State University of Londrina, Londrina, Brazil.
FAU - Sano, Ayako
AU  - Sano A
AD  - Department of Animal Sciences, University of the Ryukyus, Okinawa, Japan.
FAU - Itano, Eiko N
AU  - Itano EN
AUID- ORCID: 0000-0002-0159-5494
AD  - State University of Londrina, Londrina, Brazil.
LA  - eng
GR  - Universidade Estadual de Londrina/
GR  - Ministerio da Educacao/
GR  - Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/
GR  - Fundacao Araucaria/
GR  - Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/
PT  - Journal Article
DEP - 20180715
PL  - Germany
TA  - Mycoses
JT  - Mycoses
JID - 8805008
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antigens, Fungal)
SB  - IM
MH  - Animals
MH  - Antibodies, Fungal/analysis/*immunology
MH  - Antigens, Fungal/analysis/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Immunoblotting
MH  - Male
MH  - Paracoccidioides/classification/genetics/immunology/*isolation & purification
MH  - Paracoccidioidomycosis/diagnosis/*microbiology
MH  - Rabbits
OTO - NOTNLM
OT  - ELISA
OT  - cell free antigen
OT  - hyperimmune rabbit serum
OT  - immune response
OT  - immunoblot
OT  - paracoccidioidomycosis
EDAT- 2018/06/26 06:00
MHDA- 2018/12/19 06:00
CRDT- 2018/06/26 06:00
PHST- 2018/01/25 00:00 [received]
PHST- 2018/05/29 00:00 [revised]
PHST- 2018/06/14 00:00 [accepted]
PHST- 2018/06/26 06:00 [pubmed]
PHST- 2018/12/19 06:00 [medline]
PHST- 2018/06/26 06:00 [entrez]
AID - 10.1111/myc.12819 [doi]
PST - ppublish
SO  - Mycoses. 2018 Nov;61(11):826-832. doi: 10.1111/myc.12819. Epub 2018 Jul 15.